Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen
<i>Leptosphaeria biglobosa</i> is a less virulent <i>Leptosphaeria</i> spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent <i>L. biglobosa</i> can boost the resistance of canola plants again...
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/7/996 |
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| author | Kaluhannadige Rasanie Eranka Padmathilake Wannakuwattewaduge Gerard Dilantha Fernando |
| author_facet | Kaluhannadige Rasanie Eranka Padmathilake Wannakuwattewaduge Gerard Dilantha Fernando |
| author_sort | Kaluhannadige Rasanie Eranka Padmathilake |
| collection | DOAJ |
| description | <i>Leptosphaeria biglobosa</i> is a less virulent <i>Leptosphaeria</i> spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent <i>L. biglobosa</i> can boost the resistance of canola plants against the highly virulent <i>L. maculans</i>. The objective of this study was to confirm the effectiveness of <i>L. biglobosa</i> as a biocontrol agent against <i>L. maculans</i> utilizing morphology, fluorescence microscopy, gene quantification, and transcriptomic analysis. The in planta development of two <i>Leptosphaeria</i> species inoculated at different time points was assessed using fluorescent protein-tagged isolates which are GFP-tagged <i>L. maculans</i> and DsRed-tagged <i>L. biglobosa</i>. The growth inhibition of <i>L. maculans</i> by pre-and co-inoculated <i>L. biglobosa</i> was supported by no lesion development on cotyledons and no or weak fluorescence protein-tagged mycelia under the confocal microscope. The host defense-related genes, <i>WRKY33</i>, <i>PR1</i>, <i>APX6</i>, and <i>CHI,</i> were upregulated in <i>L. biglobosa</i> inoculated Westar cotyledons compared to <i>L. maculans</i> inoculated cotyledons. The quantification of each pathogen through qPCR assay and gene expressions analysis on host defense-related genes by RT-qPCR confirmed the potential of <i>L. biglobosa</i> “brassicae’ in the management of the blackleg disease pathogen, <i>L. maculans</i> ‘brassicae’, in canola. |
| first_indexed | 2024-03-09T11:30:14Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:30:14Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-1815e824d7ec4d17b7b9d0949a2623cc2023-11-30T23:52:03ZengMDPI AGPlants2223-77472022-04-0111799610.3390/plants11070996Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg PathogenKaluhannadige Rasanie Eranka Padmathilake0Wannakuwattewaduge Gerard Dilantha Fernando1Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada<i>Leptosphaeria biglobosa</i> is a less virulent <i>Leptosphaeria</i> spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent <i>L. biglobosa</i> can boost the resistance of canola plants against the highly virulent <i>L. maculans</i>. The objective of this study was to confirm the effectiveness of <i>L. biglobosa</i> as a biocontrol agent against <i>L. maculans</i> utilizing morphology, fluorescence microscopy, gene quantification, and transcriptomic analysis. The in planta development of two <i>Leptosphaeria</i> species inoculated at different time points was assessed using fluorescent protein-tagged isolates which are GFP-tagged <i>L. maculans</i> and DsRed-tagged <i>L. biglobosa</i>. The growth inhibition of <i>L. maculans</i> by pre-and co-inoculated <i>L. biglobosa</i> was supported by no lesion development on cotyledons and no or weak fluorescence protein-tagged mycelia under the confocal microscope. The host defense-related genes, <i>WRKY33</i>, <i>PR1</i>, <i>APX6</i>, and <i>CHI,</i> were upregulated in <i>L. biglobosa</i> inoculated Westar cotyledons compared to <i>L. maculans</i> inoculated cotyledons. The quantification of each pathogen through qPCR assay and gene expressions analysis on host defense-related genes by RT-qPCR confirmed the potential of <i>L. biglobosa</i> “brassicae’ in the management of the blackleg disease pathogen, <i>L. maculans</i> ‘brassicae’, in canola.https://www.mdpi.com/2223-7747/11/7/996<i>Brassica napus</i><i>Leptosphaeria maculans</i><i>Leptosphaeria biglobosa</i>biological control |
| spellingShingle | Kaluhannadige Rasanie Eranka Padmathilake Wannakuwattewaduge Gerard Dilantha Fernando Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen Plants <i>Brassica napus</i> <i>Leptosphaeria maculans</i> <i>Leptosphaeria biglobosa</i> biological control |
| title | Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen |
| title_full | Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen |
| title_fullStr | Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen |
| title_full_unstemmed | Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen |
| title_short | Less Virulent <i>Leptosphaeria biglobosa</i> Immunizes the Canola Plant to Resist Highly Virulent <i>L. maculans,</i> the Blackleg Pathogen |
| title_sort | less virulent i leptosphaeria biglobosa i immunizes the canola plant to resist highly virulent i l maculans i the blackleg pathogen |
| topic | <i>Brassica napus</i> <i>Leptosphaeria maculans</i> <i>Leptosphaeria biglobosa</i> biological control |
| url | https://www.mdpi.com/2223-7747/11/7/996 |
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