Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis
Background: As a wound dressing and barrier membrane, surface modification of polycaprolactone (PCL) is needed in order to achieve better biological activities. Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue regeneration promise. Silver nanoparticles (Ag) have been sugge...
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| Format: | Article |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.699802/full |
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| author | Haiping Lu Yi Zhang Shan Xiong Yinghong Zhou Yinghong Zhou Lan Xiao Lan Xiao Yaping Ma Yin Xiao Yin Xiao Xin Wang Xin Wang Xin Wang |
| author_facet | Haiping Lu Yi Zhang Shan Xiong Yinghong Zhou Yinghong Zhou Lan Xiao Lan Xiao Yaping Ma Yin Xiao Yin Xiao Xin Wang Xin Wang Xin Wang |
| author_sort | Haiping Lu |
| collection | DOAJ |
| description | Background: As a wound dressing and barrier membrane, surface modification of polycaprolactone (PCL) is needed in order to achieve better biological activities. Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue regeneration promise. Silver nanoparticles (Ag) have been suggested as the surface modification technique for various medical devices.Materials and Methods: Ag and human bone marrow MSC (hBMSC)-derived exosomes (MSCs-exo) were used to modify the PCL scaffold. The impact of different scaffolds on immune cells and MSC proliferation and differentiation was further evaluated.Results: MSCs-exo exhibited cup-shaped morphology with a diameter around 100 nm. MSCs-exo were enriched with exosome marker CD81 and showed good internalization into recipient cells. 200 ng/ml Ag nanoparticles and MSCs-exo were further used to modify the PCL scaffold. The internalization study further indicated a similar releasing pattern of exosomes from Ag/MSCs-exo hybrid scaffolds into RAW264.7 and hBMSCs at 12 and 24 h, respectively. Macrophages play an important role during different stages of bone regeneration. The MTT and confocal microscopy study demonstrated no significant toxicity of exosome and/or Ag hybrid scaffolds for macrophages and MSCs. Inflammatory macrophages were further used to mimic the inflammatory environment. A mixed population of elongated and round morphology was noted in the exosome and Ag hybrid group, in which the proinflammatory genes and secretion of IL-6 and TNF-α were significantly reduced. In addition, the exosome and Ag hybrid scaffolds could significantly boost the osteogenic differentiation of hBMSCs.Discussion: This study highlights the possibility of using Ag nanoparticles and MSCs-exo to modify the PCL scaffold, thus providing new insight into the development of the novel immunomodulatory biomembrane. |
| first_indexed | 2024-12-17T19:38:07Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-17T19:38:07Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Chemistry |
| spelling | doaj.art-18192a928695436490dd02f04f4f782b2022-12-21T21:35:06ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-08-01910.3389/fchem.2021.699802699802Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and OsteogenesisHaiping Lu0Yi Zhang1Shan Xiong2Yinghong Zhou3Yinghong Zhou4Lan Xiao5Lan Xiao6Yaping Ma7Yin Xiao8Yin Xiao9Xin Wang10Xin Wang11Xin Wang12Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaDepartment of Hygiene Toxicology, School of Public Health, Zunyi Medical University, Zunyi, ChinaDepartment of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, AustraliaThe Australia−China Centre for Tissue Engineering and Regenerative Medicine (ACCTERM), Brisbane, QLD, AustraliaInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, AustraliaThe Australia−China Centre for Tissue Engineering and Regenerative Medicine (ACCTERM), Brisbane, QLD, AustraliaDepartment of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, AustraliaThe Australia−China Centre for Tissue Engineering and Regenerative Medicine (ACCTERM), Brisbane, QLD, AustraliaDepartment of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, AustraliaThe Australia−China Centre for Tissue Engineering and Regenerative Medicine (ACCTERM), Brisbane, QLD, AustraliaBackground: As a wound dressing and barrier membrane, surface modification of polycaprolactone (PCL) is needed in order to achieve better biological activities. Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue regeneration promise. Silver nanoparticles (Ag) have been suggested as the surface modification technique for various medical devices.Materials and Methods: Ag and human bone marrow MSC (hBMSC)-derived exosomes (MSCs-exo) were used to modify the PCL scaffold. The impact of different scaffolds on immune cells and MSC proliferation and differentiation was further evaluated.Results: MSCs-exo exhibited cup-shaped morphology with a diameter around 100 nm. MSCs-exo were enriched with exosome marker CD81 and showed good internalization into recipient cells. 200 ng/ml Ag nanoparticles and MSCs-exo were further used to modify the PCL scaffold. The internalization study further indicated a similar releasing pattern of exosomes from Ag/MSCs-exo hybrid scaffolds into RAW264.7 and hBMSCs at 12 and 24 h, respectively. Macrophages play an important role during different stages of bone regeneration. The MTT and confocal microscopy study demonstrated no significant toxicity of exosome and/or Ag hybrid scaffolds for macrophages and MSCs. Inflammatory macrophages were further used to mimic the inflammatory environment. A mixed population of elongated and round morphology was noted in the exosome and Ag hybrid group, in which the proinflammatory genes and secretion of IL-6 and TNF-α were significantly reduced. In addition, the exosome and Ag hybrid scaffolds could significantly boost the osteogenic differentiation of hBMSCs.Discussion: This study highlights the possibility of using Ag nanoparticles and MSCs-exo to modify the PCL scaffold, thus providing new insight into the development of the novel immunomodulatory biomembrane.https://www.frontiersin.org/articles/10.3389/fchem.2021.699802/fullexosomemesenchymal stem cellsAgPCL hybrid scaffoldosteogenesis |
| spellingShingle | Haiping Lu Yi Zhang Shan Xiong Yinghong Zhou Yinghong Zhou Lan Xiao Lan Xiao Yaping Ma Yin Xiao Yin Xiao Xin Wang Xin Wang Xin Wang Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis Frontiers in Chemistry exosome mesenchymal stem cells Ag PCL hybrid scaffold osteogenesis |
| title | Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis |
| title_full | Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis |
| title_fullStr | Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis |
| title_full_unstemmed | Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis |
| title_short | Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis |
| title_sort | modulatory role of silver nanoparticles and mesenchymal stem cell derived exosome modified barrier membrane on macrophages and osteogenesis |
| topic | exosome mesenchymal stem cells Ag PCL hybrid scaffold osteogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.699802/full |
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