Freehand scanning photoacoustic microscopy with simultaneous localization and mapping
Optical-resolution photoacoustic microscopy offers high-resolution, label-free hemodynamic and functional imaging to many biomedical applications. However, long-standing technical barriers, such as limited field of view, bulky scanning probes, and slow imaging speed, have limited the application of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000763 |
| _version_ | 1811179046119145472 |
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| author | Jiangbo Chen Yachao Zhang Jingyi Zhu Xu Tang Lidai Wang |
| author_facet | Jiangbo Chen Yachao Zhang Jingyi Zhu Xu Tang Lidai Wang |
| author_sort | Jiangbo Chen |
| collection | DOAJ |
| description | Optical-resolution photoacoustic microscopy offers high-resolution, label-free hemodynamic and functional imaging to many biomedical applications. However, long-standing technical barriers, such as limited field of view, bulky scanning probes, and slow imaging speed, have limited the application of optical-resolution photoacoustic microscopy. Here, we present freehand scanning photoacoustic microscopy (FS-PAM) that can flexibly image various anatomical sites. We develop a compact handheld photoacoustic probe to acquire 3D images with high speed, and great flexibility. The high scanning speed not only enables video camera mode imaging but also allows for the first implementation of simultaneous localization and mapping (SLAM) in photoacoustic microscopy. We demonstrate fast in vivo imaging of some mouse organs, and human oral mucosa. The high imaging speed greatly reduces motion artifacts and distortions from tissue moving, breathing, and unintended handshaking. We demonstrate small-lesion localization in a large region of the brain. FS-PAM offers a flexible high-speed imaging tool with an extendable field of view, enabling more biomedical imaging applications. |
| first_indexed | 2024-04-11T06:28:02Z |
| format | Article |
| id | doaj.art-181f3fcfac1f4578a34be7eb8fe6cc6f |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-11T06:28:02Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-181f3fcfac1f4578a34be7eb8fe6cc6f2022-12-22T04:40:15ZengElsevierPhotoacoustics2213-59792022-12-0128100411Freehand scanning photoacoustic microscopy with simultaneous localization and mappingJiangbo Chen0Yachao Zhang1Jingyi Zhu2Xu Tang3Lidai Wang4Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of China; City University of Hong Kong Shenzhen Research Institute, Yuexing Yi Dao, Shenzhen, Guang Dong 518057, China; Corresponding author at: Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong Special Administrative Region of China.Optical-resolution photoacoustic microscopy offers high-resolution, label-free hemodynamic and functional imaging to many biomedical applications. However, long-standing technical barriers, such as limited field of view, bulky scanning probes, and slow imaging speed, have limited the application of optical-resolution photoacoustic microscopy. Here, we present freehand scanning photoacoustic microscopy (FS-PAM) that can flexibly image various anatomical sites. We develop a compact handheld photoacoustic probe to acquire 3D images with high speed, and great flexibility. The high scanning speed not only enables video camera mode imaging but also allows for the first implementation of simultaneous localization and mapping (SLAM) in photoacoustic microscopy. We demonstrate fast in vivo imaging of some mouse organs, and human oral mucosa. The high imaging speed greatly reduces motion artifacts and distortions from tissue moving, breathing, and unintended handshaking. We demonstrate small-lesion localization in a large region of the brain. FS-PAM offers a flexible high-speed imaging tool with an extendable field of view, enabling more biomedical imaging applications.http://www.sciencedirect.com/science/article/pii/S2213597922000763Photoacoustic microscopyOptical-resolutionFreehand-scanningExpendable field of view |
| spellingShingle | Jiangbo Chen Yachao Zhang Jingyi Zhu Xu Tang Lidai Wang Freehand scanning photoacoustic microscopy with simultaneous localization and mapping Photoacoustics Photoacoustic microscopy Optical-resolution Freehand-scanning Expendable field of view |
| title | Freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| title_full | Freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| title_fullStr | Freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| title_full_unstemmed | Freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| title_short | Freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| title_sort | freehand scanning photoacoustic microscopy with simultaneous localization and mapping |
| topic | Photoacoustic microscopy Optical-resolution Freehand-scanning Expendable field of view |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000763 |
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