Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the bi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/20/6258 |
| _version_ | 1797513666844688384 |
|---|---|
| author | Miriam Spreda Nicole Hauptmann Veronika Lehner Christoph Biehl Klaus Liefeith Katrin Susanne Lips |
| author_facet | Miriam Spreda Nicole Hauptmann Veronika Lehner Christoph Biehl Klaus Liefeith Katrin Susanne Lips |
| author_sort | Miriam Spreda |
| collection | DOAJ |
| description | In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity. |
| first_indexed | 2024-03-10T06:19:50Z |
| format | Article |
| id | doaj.art-182271e977e44533aa5757c72ac1d807 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:19:50Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-182271e977e44533aa5757c72ac1d8072023-11-22T19:20:31ZengMDPI AGMolecules1420-30492021-10-012620625810.3390/molecules26206258Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast ActivityMiriam Spreda0Nicole Hauptmann1Veronika Lehner2Christoph Biehl3Klaus Liefeith4Katrin Susanne Lips5Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, GermanyDepartment of Biomaterials, Institute for Bioprocessing and Analytical Measurement Techniques e.V. (iba), Rosenhof, 37308 Heilbad Heiligenstadt, GermanyExperimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital of Giessen-Marburg GmbH, Campus Giessen, Rudolf-Buchheim-Strasse 7, 35392 Giessen, GermanyDepartment of Biomaterials, Institute for Bioprocessing and Analytical Measurement Techniques e.V. (iba), Rosenhof, 37308 Heilbad Heiligenstadt, GermanyExperimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, GermanyIn the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity.https://www.mdpi.com/1420-3049/26/20/62583D-printingosteoclastsmesenchymal stromal cellstartrate-resistant acid phosphatasemRNA expressionimplant |
| spellingShingle | Miriam Spreda Nicole Hauptmann Veronika Lehner Christoph Biehl Klaus Liefeith Katrin Susanne Lips Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity Molecules 3D-printing osteoclasts mesenchymal stromal cells tartrate-resistant acid phosphatase mRNA expression implant |
| title | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
| title_full | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
| title_fullStr | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
| title_full_unstemmed | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
| title_short | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
| title_sort | porous 3d scaffolds enhance msc vitality and reduce osteoclast activity |
| topic | 3D-printing osteoclasts mesenchymal stromal cells tartrate-resistant acid phosphatase mRNA expression implant |
| url | https://www.mdpi.com/1420-3049/26/20/6258 |
| work_keys_str_mv | AT miriamspreda porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity AT nicolehauptmann porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity AT veronikalehner porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity AT christophbiehl porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity AT klausliefeith porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity AT katrinsusannelips porous3dscaffoldsenhancemscvitalityandreduceosteoclastactivity |