Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue
ABSTRACT: CCAAT/enhancer binding protein zeta (C/EBPZ) was differentially expressed in abdominal adipose tissues of fat and lean broilers and regulated adipogenesis in chicken. The objective of this study was to elucidate the transcriptional regulation of C/EBPZ gene in chicken adipose tissue. A 2,0...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001196 |
| _version_ | 1797256462457634816 |
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| author | Lingyu Gao Yingjun Wang Qin Gao Yuechan Chen Zhiwei Zhang |
| author_facet | Lingyu Gao Yingjun Wang Qin Gao Yuechan Chen Zhiwei Zhang |
| author_sort | Lingyu Gao |
| collection | DOAJ |
| description | ABSTRACT: CCAAT/enhancer binding protein zeta (C/EBPZ) was differentially expressed in abdominal adipose tissues of fat and lean broilers and regulated adipogenesis in chicken. The objective of this study was to elucidate the transcriptional regulation of C/EBPZ gene in chicken adipose tissue. A 2,031-base pair (bp) chicken C/EBPZ sequence (2,025 nucleotides upstream to 6 nucleotides downstream from the initiator codon, -2,025/+6) was studied. The sequence exhibited a significant promoter activity (P < 0.05) and had some cis-acting elements, notably, a core promoter was identified in nucleotides −94 to +6. Additionally, DNA pull-down assay showed that proteins interacted with chicken C/EBPZ promoter (−173/+6) in preadipocytes were implicated in transcription, post-transcriptional regulation and translation. In addition, KLF2 facilitated the activities of chicken C/EBPZ promoter (−2,025/+6, −1,409/+6, −793/+6, −485/+6, −173/+6, and −94/+6) in preadipocytes (P < 0.05). The expression levels of KLF2 and C/EBPZ in chicken abdominal adipose tissue were substantially associated (r = 0.5978278, P < 0.0001), and KLF2 increased C/EBPZ expression in vitro (P < 0.05). Additionally, chromatin immunoprecipitation (ChIP)-PCR analysis revealed that KLF2 has the ability to interact with the chicken C/EBPZ promoter regions at least at the positions −1,245/−1,048 and −571/−397. Mutation analysis showed that the CGCAGCGCCCG motif located in the chicken C/EBPZ promoter at positions -45 to -35 is involved in regulating transcription and facilitates trans activation by KLF2. These results provided some information of transcription control of C/EBPZ in chicken adipose tissue. |
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| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:08Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-1824aadf55c24284a4add836f58f6b0e2024-03-20T06:08:02ZengElsevierPoultry Science0032-57912024-04-011034103540Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissueLingyu Gao0Yingjun Wang1Qin Gao2Yuechan Chen3Zhiwei Zhang4Department of Histology and Embryology, Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, PR China; Key Medical Laboratory of Stem Cell Transformation and Application, The First People's Hospital of Zhengzhou, Zhengzhou, Henan, 450000, PR ChinaDepartment of Histology and Embryology, Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, PR ChinaDepartment of Histology and Embryology, Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, PR ChinaDepartment of Reproductive Medicine, The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, PR ChinaDepartment of Histology and Embryology, Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, PR China; Corresponding author:ABSTRACT: CCAAT/enhancer binding protein zeta (C/EBPZ) was differentially expressed in abdominal adipose tissues of fat and lean broilers and regulated adipogenesis in chicken. The objective of this study was to elucidate the transcriptional regulation of C/EBPZ gene in chicken adipose tissue. A 2,031-base pair (bp) chicken C/EBPZ sequence (2,025 nucleotides upstream to 6 nucleotides downstream from the initiator codon, -2,025/+6) was studied. The sequence exhibited a significant promoter activity (P < 0.05) and had some cis-acting elements, notably, a core promoter was identified in nucleotides −94 to +6. Additionally, DNA pull-down assay showed that proteins interacted with chicken C/EBPZ promoter (−173/+6) in preadipocytes were implicated in transcription, post-transcriptional regulation and translation. In addition, KLF2 facilitated the activities of chicken C/EBPZ promoter (−2,025/+6, −1,409/+6, −793/+6, −485/+6, −173/+6, and −94/+6) in preadipocytes (P < 0.05). The expression levels of KLF2 and C/EBPZ in chicken abdominal adipose tissue were substantially associated (r = 0.5978278, P < 0.0001), and KLF2 increased C/EBPZ expression in vitro (P < 0.05). Additionally, chromatin immunoprecipitation (ChIP)-PCR analysis revealed that KLF2 has the ability to interact with the chicken C/EBPZ promoter regions at least at the positions −1,245/−1,048 and −571/−397. Mutation analysis showed that the CGCAGCGCCCG motif located in the chicken C/EBPZ promoter at positions -45 to -35 is involved in regulating transcription and facilitates trans activation by KLF2. These results provided some information of transcription control of C/EBPZ in chicken adipose tissue.http://www.sciencedirect.com/science/article/pii/S0032579124001196Chickenadipose tissuetranscription regulationpromoterCCAAT/enhancer binding protein zeta |
| spellingShingle | Lingyu Gao Yingjun Wang Qin Gao Yuechan Chen Zhiwei Zhang Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue Poultry Science Chicken adipose tissue transcription regulation promoter CCAAT/enhancer binding protein zeta |
| title | Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue |
| title_full | Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue |
| title_fullStr | Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue |
| title_full_unstemmed | Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue |
| title_short | Transcriptional control of CCAAT/enhancer binding protein zeta gene in chicken adipose tissue |
| title_sort | transcriptional control of ccaat enhancer binding protein zeta gene in chicken adipose tissue |
| topic | Chicken adipose tissue transcription regulation promoter CCAAT/enhancer binding protein zeta |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001196 |
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