A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patient...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.956044/full |
| _version_ | 1811225290696818688 |
|---|---|
| author | Weiliang Zeng Wenli Liao Wenli Liao Yajie Zhao Lingbo Wang Hongyun Shu Huaiyu Jia Tao Chen Ying Zhang Tieli Zhou Qing Wu |
| author_facet | Weiliang Zeng Wenli Liao Wenli Liao Yajie Zhao Lingbo Wang Hongyun Shu Huaiyu Jia Tao Chen Ying Zhang Tieli Zhou Qing Wu |
| author_sort | Weiliang Zeng |
| collection | DOAJ |
| description | Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patients or a specific population so as to rapidly implement infection control measures to limit their transmission. In this study, we developed a Salmonella-Shigella (SS) CZA-selective medium and assessed its performance to screen for clinical CZA-resistant CRE isolates in both pure-strain specimens and stool samples. A total of 150 non-duplicated isolates, including 75 CZA-susceptible and 75 CZA-resistant CRE pathogens, were tested by using the broth microdilution method and the SS CZA medium, respectively. The bacterial suspensions were serially diluted in the SS CZA medium, which showed excellent screening performance in both pure CZA-resistant CRE strain and the stool samples with the lowest detection limit of 101-102 and 101-103 CFU/ml, respectively. Notably, none of the susceptible isolates showed growth even at the highest dilution concentration of 108 CFU/ml. Most importantly, the SS CZA medium demonstrated excellent performance in screening simulated clinical polymicrobial specimens. Moreover, its screening performance was unaffected by the different resistance determinants for tested isolates. Cumulatively, our data suggest that the SS CZA medium can be used as a promising selective medium to screen CZA-resistant CRE, irrespective of their resistance mechanisms. |
| first_indexed | 2024-04-12T09:04:30Z |
| format | Article |
| id | doaj.art-18251132d68b434f9bc2ce324a392c42 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T09:04:30Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-18251132d68b434f9bc2ce324a392c422022-12-22T03:39:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.956044956044A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant EnterobacteralesWeiliang Zeng0Wenli Liao1Wenli Liao2Yajie Zhao3Lingbo Wang4Hongyun Shu5Huaiyu Jia6Tao Chen7Ying Zhang8Tieli Zhou9Qing Wu10Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Laboratory, Yongzhou Central Hospital, Yongzhou, ChinaDepartment of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaCeftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patients or a specific population so as to rapidly implement infection control measures to limit their transmission. In this study, we developed a Salmonella-Shigella (SS) CZA-selective medium and assessed its performance to screen for clinical CZA-resistant CRE isolates in both pure-strain specimens and stool samples. A total of 150 non-duplicated isolates, including 75 CZA-susceptible and 75 CZA-resistant CRE pathogens, were tested by using the broth microdilution method and the SS CZA medium, respectively. The bacterial suspensions were serially diluted in the SS CZA medium, which showed excellent screening performance in both pure CZA-resistant CRE strain and the stool samples with the lowest detection limit of 101-102 and 101-103 CFU/ml, respectively. Notably, none of the susceptible isolates showed growth even at the highest dilution concentration of 108 CFU/ml. Most importantly, the SS CZA medium demonstrated excellent performance in screening simulated clinical polymicrobial specimens. Moreover, its screening performance was unaffected by the different resistance determinants for tested isolates. Cumulatively, our data suggest that the SS CZA medium can be used as a promising selective medium to screen CZA-resistant CRE, irrespective of their resistance mechanisms.https://www.frontiersin.org/articles/10.3389/fmicb.2022.956044/fullceftazidimeavibactammediumCREresistancescreening |
| spellingShingle | Weiliang Zeng Wenli Liao Wenli Liao Yajie Zhao Lingbo Wang Hongyun Shu Huaiyu Jia Tao Chen Ying Zhang Tieli Zhou Qing Wu A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales Frontiers in Microbiology ceftazidime avibactam medium CRE resistance screening |
| title | A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales |
| title_full | A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales |
| title_fullStr | A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales |
| title_full_unstemmed | A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales |
| title_short | A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales |
| title_sort | selective medium for screening ceftazidime avibactam resistance in carbapenem resistant enterobacterales |
| topic | ceftazidime avibactam medium CRE resistance screening |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.956044/full |
| work_keys_str_mv | AT weiliangzeng aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT wenliliao aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT wenliliao aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT yajiezhao aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT lingbowang aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT hongyunshu aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT huaiyujia aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT taochen aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT yingzhang aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT tielizhou aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT qingwu aselectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT weiliangzeng selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT wenliliao selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT wenliliao selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT yajiezhao selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT lingbowang selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT hongyunshu selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT huaiyujia selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT taochen selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT yingzhang selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT tielizhou selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales AT qingwu selectivemediumforscreeningceftazidimeavibactamresistanceincarbapenemresistantenterobacterales |