Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i>
<i>Ectropis obliqua</i> is a destructive masticatory pest in China’s tea gardens. <i>Beauveria bassiana</i> as microbial insecticides can effectively control <i>E. obliqua</i> larvae; however, the immune response of this insect infected by <i>B. bassiana<...
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MDPI AG
2022-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/3/225 |
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| author | Yanhua Long Tian Gao Song Liu Yong Zhang Xiayu Li Linlin Zhou Qingqing Su Letian Xu Yunqiu Yang |
| author_facet | Yanhua Long Tian Gao Song Liu Yong Zhang Xiayu Li Linlin Zhou Qingqing Su Letian Xu Yunqiu Yang |
| author_sort | Yanhua Long |
| collection | DOAJ |
| description | <i>Ectropis obliqua</i> is a destructive masticatory pest in China’s tea gardens. <i>Beauveria bassiana</i> as microbial insecticides can effectively control <i>E. obliqua</i> larvae; however, the immune response of this insect infected by <i>B. bassiana</i> are largely unknown. Here, after isolating a highly virulent strain of <i>B. bassiana</i> from <i>E. obliqua</i>, the changes in gene expression among different tissues, including hemocytes and fat bodies, of <i>E. obliqua</i> larvae infected by the entomopathogen were investigated using transcriptome sequencing. A total of 5877 co-expressed differentially expressed genes (DEGs) were identified in hemocytes and fat bodies, of which 5826 were up-regulated in hemocytes and 5784 were up-regulated in fat bodies. We identified 249 immunity-related genes, including pattern recognition receptors, immune effectors, signal modulators, and members of immune pathways. A quantitative real-time PCR analysis confirmed that several pattern recognition receptors were upregulated in hemocytes and fat bodies; however, others were downregulated. The investigated immune effectors (<i>ATT</i> and <i>PPO-1</i>) were suppressed. The results showed that there were tissue differences in the expression of immune genes. This study provides a large number of immunity-related gene sequences from <i>E. obliqua</i> after being infected by <i>B. bassiana</i>, furthering the understanding of the molecular mechanisms of <i>E. obliqua</i> defenses against <i>B. bassiana</i>. |
| first_indexed | 2024-03-09T19:39:10Z |
| format | Article |
| id | doaj.art-182a2b1423854cfcb52ae5475081e12c |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T19:39:10Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-182a2b1423854cfcb52ae5475081e12c2023-11-24T01:42:55ZengMDPI AGInsects2075-44502022-02-0113322510.3390/insects13030225Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i>Yanhua Long0Tian Gao1Song Liu2Yong Zhang3Xiayu Li4Linlin Zhou5Qingqing Su6Letian Xu7Yunqiu Yang8School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China<i>Ectropis obliqua</i> is a destructive masticatory pest in China’s tea gardens. <i>Beauveria bassiana</i> as microbial insecticides can effectively control <i>E. obliqua</i> larvae; however, the immune response of this insect infected by <i>B. bassiana</i> are largely unknown. Here, after isolating a highly virulent strain of <i>B. bassiana</i> from <i>E. obliqua</i>, the changes in gene expression among different tissues, including hemocytes and fat bodies, of <i>E. obliqua</i> larvae infected by the entomopathogen were investigated using transcriptome sequencing. A total of 5877 co-expressed differentially expressed genes (DEGs) were identified in hemocytes and fat bodies, of which 5826 were up-regulated in hemocytes and 5784 were up-regulated in fat bodies. We identified 249 immunity-related genes, including pattern recognition receptors, immune effectors, signal modulators, and members of immune pathways. A quantitative real-time PCR analysis confirmed that several pattern recognition receptors were upregulated in hemocytes and fat bodies; however, others were downregulated. The investigated immune effectors (<i>ATT</i> and <i>PPO-1</i>) were suppressed. The results showed that there were tissue differences in the expression of immune genes. This study provides a large number of immunity-related gene sequences from <i>E. obliqua</i> after being infected by <i>B. bassiana</i>, furthering the understanding of the molecular mechanisms of <i>E. obliqua</i> defenses against <i>B. bassiana</i>.https://www.mdpi.com/2075-4450/13/3/225<i>Ectropis obliqua</i><i>Beauveria bassiana</i>hemocytesfat body tissuesimmunity-related genes |
| spellingShingle | Yanhua Long Tian Gao Song Liu Yong Zhang Xiayu Li Linlin Zhou Qingqing Su Letian Xu Yunqiu Yang Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> Insects <i>Ectropis obliqua</i> <i>Beauveria bassiana</i> hemocytes fat body tissues immunity-related genes |
| title | Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> |
| title_full | Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> |
| title_fullStr | Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> |
| title_full_unstemmed | Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> |
| title_short | Analysis of the Humoral Immunal Response Transcriptome of <i>Ectropis obliqua</i> Infected by <i>Beauveria bassiana</i> |
| title_sort | analysis of the humoral immunal response transcriptome of i ectropis obliqua i infected by i beauveria bassiana i |
| topic | <i>Ectropis obliqua</i> <i>Beauveria bassiana</i> hemocytes fat body tissues immunity-related genes |
| url | https://www.mdpi.com/2075-4450/13/3/225 |
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