Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart
Objectives This study aimed to evaluate the cardioprotective effects and mechanism of scutellarin (Scu) on heart ischaemia/reperfusion (I/R) injury by using an isolated Langendorff rat heart model. Methods Myocardial function was evaluated via measurements of cardiac haemodynamic parameters. Myocard...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-07-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X231192289 |
| _version_ | 1797752179419774976 |
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| author | Daoxu Qu Peiwen Feng Xinjie Zhang Mingjie Zhou |
| author_facet | Daoxu Qu Peiwen Feng Xinjie Zhang Mingjie Zhou |
| author_sort | Daoxu Qu |
| collection | DOAJ |
| description | Objectives This study aimed to evaluate the cardioprotective effects and mechanism of scutellarin (Scu) on heart ischaemia/reperfusion (I/R) injury by using an isolated Langendorff rat heart model. Methods Myocardial function was evaluated via measurements of cardiac haemodynamic parameters. Myocardial infarct size was macroscopically estimated by using TTC staining. The release of creatine kinase (CK) and lactate dehydrogenase (LDH) was used to evaluate cardiac injury. GSH/GSSG, SOD, and MDA were determined as being indicators of oxidative stress. CRP, IL-6, and TNF-α were also analysed to assess inflammation in the heart tissues. Cardiomyocyte apoptosis was quantified by using a TUNEL kit. PPARγ, Nrf2, and NF-κB were assayed via Western blotting. Results The results indicated that I/R induced significant cardiac dysfunction, myocardial infarct, and apoptosis, as well as decreasing the GSH/GSSG ratio, SOD activity, Nrf2 protein expression, and PPARγ protein expression, but increased levels of TNF-α, MDA, CRP, IL-6, and NF-κB. Conclusion These data suggest that Scu attenuates cardiac I/R. |
| first_indexed | 2024-03-12T16:59:25Z |
| format | Article |
| id | doaj.art-182d3458d27e4886bd946746696d137d |
| institution | Directory Open Access Journal |
| issn | 2058-7392 |
| language | English |
| last_indexed | 2024-03-12T16:59:25Z |
| publishDate | 2023-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-182d3458d27e4886bd946746696d137d2023-08-07T17:03:32ZengSAGE PublishingEuropean Journal of Inflammation2058-73922023-07-012110.1177/1721727X231192289Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heartDaoxu QuPeiwen FengXinjie ZhangMingjie ZhouObjectives This study aimed to evaluate the cardioprotective effects and mechanism of scutellarin (Scu) on heart ischaemia/reperfusion (I/R) injury by using an isolated Langendorff rat heart model. Methods Myocardial function was evaluated via measurements of cardiac haemodynamic parameters. Myocardial infarct size was macroscopically estimated by using TTC staining. The release of creatine kinase (CK) and lactate dehydrogenase (LDH) was used to evaluate cardiac injury. GSH/GSSG, SOD, and MDA were determined as being indicators of oxidative stress. CRP, IL-6, and TNF-α were also analysed to assess inflammation in the heart tissues. Cardiomyocyte apoptosis was quantified by using a TUNEL kit. PPARγ, Nrf2, and NF-κB were assayed via Western blotting. Results The results indicated that I/R induced significant cardiac dysfunction, myocardial infarct, and apoptosis, as well as decreasing the GSH/GSSG ratio, SOD activity, Nrf2 protein expression, and PPARγ protein expression, but increased levels of TNF-α, MDA, CRP, IL-6, and NF-κB. Conclusion These data suggest that Scu attenuates cardiac I/R.https://doi.org/10.1177/1721727X231192289 |
| spellingShingle | Daoxu Qu Peiwen Feng Xinjie Zhang Mingjie Zhou Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart European Journal of Inflammation |
| title | Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart |
| title_full | Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart |
| title_fullStr | Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart |
| title_full_unstemmed | Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart |
| title_short | Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart |
| title_sort | effects of scutellarin on acute myocardial ischemia reperfusion injury in isolated rat heart |
| url | https://doi.org/10.1177/1721727X231192289 |
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