Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo
Galactooligosaccharides (GOS) are an important class of dietary prebiotics that exert beneficial effects on intestinal microbiota and gut barrier function. In this study, high-purity GOS (HP-GOS) were investigated in vitro and in vivo and confirmed as prebiotic ingredients in rat diet. HP-GOS were s...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2016-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/236560 |
| _version_ | 1797207989511258112 |
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| author | Ki Bae Hong Jae Hwan Kim Hyuk Kon Kwon Sung Hee Han Yooheon Park Hyung Joo Suh |
| author_facet | Ki Bae Hong Jae Hwan Kim Hyuk Kon Kwon Sung Hee Han Yooheon Park Hyung Joo Suh |
| author_sort | Ki Bae Hong |
| collection | DOAJ |
| description | Galactooligosaccharides (GOS) are an important class of dietary prebiotics that exert beneficial effects on intestinal microbiota and gut barrier function. In this study, high-purity GOS (HP-GOS) were investigated in vitro and in vivo and confirmed as prebiotic ingredients in rat diet. HP-GOS were successfully produced using a two-step process, enzymatic hydrolysis and fermentation by yeast. They were found to serve as a good substrate and carbon source for supporting the growth of probiotic bacteria more effectively than other commercial GOS. Following administration of 1 % (by mass) of HP-GOS to rats, the growth of Bifidobacterium bifidum and B. longum in the gut increased most rapidly up to 12 h, and thereafter the increase was slow. Therefore, 1 % HP-GOS was found to be acceptable for the growth of probiotic bacteria. Groups of animals that were orally administered HP-GOS and bifidobacteria during the study, and the group administered HP-GOS during the 2nd (days 13–15) and 4th (days 28–30) period of the study had significantly (p<0.05) higher numbers of bifidobacteria in faeces than groups receiving a single dose of bifidobacteria. HP-GOS affected the expression of genes encoding glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). There was a significant upregulation of GLP-1 and PYY mRNA with HP-GOS and bifidobacteria intake. We propose that the prebiotic properties of HP-GOS are potentially valuable for the production of functional foods for human consumption. |
| first_indexed | 2024-04-24T09:31:40Z |
| format | Article |
| id | doaj.art-1831dff4a6404815b4ae6c2d88b8780e |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:31:40Z |
| publishDate | 2016-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-1831dff4a6404815b4ae6c2d88b8780e2024-04-15T13:51:16ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062016-01-0154215616310.17113/ftb.54.02.16.4292Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivoKi Bae Hong0Jae Hwan Kim1Hyuk Kon Kwon2Sung Hee Han3Yooheon Park4Hyung Joo Suh5Department of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of KoreaNeo Cremar Co. Ltd., Seoul, Republic of KoreaNeo Cremar Co. Ltd., Seoul, Republic of KoreaDepartment of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of KoreaDongguk University, Research Institute of Biotechnology, Goyang, Republic of KoreaDepartment of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of KoreaGalactooligosaccharides (GOS) are an important class of dietary prebiotics that exert beneficial effects on intestinal microbiota and gut barrier function. In this study, high-purity GOS (HP-GOS) were investigated in vitro and in vivo and confirmed as prebiotic ingredients in rat diet. HP-GOS were successfully produced using a two-step process, enzymatic hydrolysis and fermentation by yeast. They were found to serve as a good substrate and carbon source for supporting the growth of probiotic bacteria more effectively than other commercial GOS. Following administration of 1 % (by mass) of HP-GOS to rats, the growth of Bifidobacterium bifidum and B. longum in the gut increased most rapidly up to 12 h, and thereafter the increase was slow. Therefore, 1 % HP-GOS was found to be acceptable for the growth of probiotic bacteria. Groups of animals that were orally administered HP-GOS and bifidobacteria during the study, and the group administered HP-GOS during the 2nd (days 13–15) and 4th (days 28–30) period of the study had significantly (p<0.05) higher numbers of bifidobacteria in faeces than groups receiving a single dose of bifidobacteria. HP-GOS affected the expression of genes encoding glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). There was a significant upregulation of GLP-1 and PYY mRNA with HP-GOS and bifidobacteria intake. We propose that the prebiotic properties of HP-GOS are potentially valuable for the production of functional foods for human consumption.https://hrcak.srce.hr/file/236560high-purity galactooligosaccharidesbifidobacteriagenes encoding GLP-1 and PYY peptides |
| spellingShingle | Ki Bae Hong Jae Hwan Kim Hyuk Kon Kwon Sung Hee Han Yooheon Park Hyung Joo Suh Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo Food Technology and Biotechnology high-purity galactooligosaccharides bifidobacteria genes encoding GLP-1 and PYY peptides |
| title | Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo |
| title_full | Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo |
| title_fullStr | Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo |
| title_full_unstemmed | Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo |
| title_short | Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo |
| title_sort | evaluation of prebiotic effects of high purity galactooligosaccharides in vitro and in vivo |
| topic | high-purity galactooligosaccharides bifidobacteria genes encoding GLP-1 and PYY peptides |
| url | https://hrcak.srce.hr/file/236560 |
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