Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes
Ruifen Su,* Xiaoli Wu,* Fengmei Ke Department of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fengmei KeDepartment of Obste...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-12-01
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| Series: | International Journal of General Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/long-non-coding-rna-hotair-expression-and-clinical-significance-in-pat-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1818279878348242944 |
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| author | Su R Wu X Ke F |
| author_facet | Su R Wu X Ke F |
| author_sort | Su R |
| collection | DOAJ |
| description | Ruifen Su,* Xiaoli Wu,* Fengmei Ke Department of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fengmei KeDepartment of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, No. 158 Wuyang Avenue, Enshi, 445000, People’s Republic of ChinaTel/Fax +86-7188222760Email ken76duan@163.comPurpose: The global incidence of gestational diabetes mellitus (GDM) is increasing year by year, and many studies have proved that long non-coding RNA (lncRNA) is involved in the regulation of GDM. The purpose of this study was to investigate the expression of HOTAIR in GDM patients and its clinical significance.Patients and Methods: Ninety-eight healthy pregnant women and 99 pregnant women diagnosed with GDM were enrolled in this study. Blood samples were collected from all participants and used for qRT-PCR analysis to determine the serum HOTAIR levels. The ROC curve was constructed to evaluate the diagnostic value of HOTAIR for GDM. Pearson correlation coefficient was used to estimate the correlation between HOTAIR and clinical indicators of patients. Logistic regression analysis was performed to evaluate the independent predictors of GDM.Results: The level of HOTAIR was augmented in GDM group compared with healthy controls. ROC curve revealed that HOTAIR as a diagnostic marker of GDM has high sensitivity and specificity. Pearson correlation coefficient showed that HOTAIR level was positively correlated with body mass index, fasting plasma glucose, 1-hour plasma glucose and 2-hour plasma glucose. Logistic regression analysis shows that HOTAIR is an independent factor of the occurrence of GDM.Conclusion: The abnormal expression of HOTAIR in pregnant women with GDM made it a potential diagnostic biomarker for GDM.Keywords: gestational diabetes mellitus, HOTAIR, diagnosis, biomarker |
| first_indexed | 2024-12-12T23:40:19Z |
| format | Article |
| id | doaj.art-18353f52f20e4844bde18e9cfae0fab6 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-12-12T23:40:19Z |
| publishDate | 2021-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-18353f52f20e4844bde18e9cfae0fab62022-12-22T00:07:13ZengDove Medical PressInternational Journal of General Medicine1178-70742021-12-01Volume 149945995071565Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational DiabetesSu RWu XKe FRuifen Su,* Xiaoli Wu,* Fengmei Ke Department of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fengmei KeDepartment of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, No. 158 Wuyang Avenue, Enshi, 445000, People’s Republic of ChinaTel/Fax +86-7188222760Email ken76duan@163.comPurpose: The global incidence of gestational diabetes mellitus (GDM) is increasing year by year, and many studies have proved that long non-coding RNA (lncRNA) is involved in the regulation of GDM. The purpose of this study was to investigate the expression of HOTAIR in GDM patients and its clinical significance.Patients and Methods: Ninety-eight healthy pregnant women and 99 pregnant women diagnosed with GDM were enrolled in this study. Blood samples were collected from all participants and used for qRT-PCR analysis to determine the serum HOTAIR levels. The ROC curve was constructed to evaluate the diagnostic value of HOTAIR for GDM. Pearson correlation coefficient was used to estimate the correlation between HOTAIR and clinical indicators of patients. Logistic regression analysis was performed to evaluate the independent predictors of GDM.Results: The level of HOTAIR was augmented in GDM group compared with healthy controls. ROC curve revealed that HOTAIR as a diagnostic marker of GDM has high sensitivity and specificity. Pearson correlation coefficient showed that HOTAIR level was positively correlated with body mass index, fasting plasma glucose, 1-hour plasma glucose and 2-hour plasma glucose. Logistic regression analysis shows that HOTAIR is an independent factor of the occurrence of GDM.Conclusion: The abnormal expression of HOTAIR in pregnant women with GDM made it a potential diagnostic biomarker for GDM.Keywords: gestational diabetes mellitus, HOTAIR, diagnosis, biomarkerhttps://www.dovepress.com/long-non-coding-rna-hotair-expression-and-clinical-significance-in-pat-peer-reviewed-fulltext-article-IJGMgestational diabetes mellitushotairdiagnosisbiomarker |
| spellingShingle | Su R Wu X Ke F Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes International Journal of General Medicine gestational diabetes mellitus hotair diagnosis biomarker |
| title | Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes |
| title_full | Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes |
| title_fullStr | Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes |
| title_full_unstemmed | Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes |
| title_short | Long Non-Coding RNA HOTAIR Expression and Clinical Significance in Patients with Gestational Diabetes |
| title_sort | long non coding rna hotair expression and clinical significance in patients with gestational diabetes |
| topic | gestational diabetes mellitus hotair diagnosis biomarker |
| url | https://www.dovepress.com/long-non-coding-rna-hotair-expression-and-clinical-significance-in-pat-peer-reviewed-fulltext-article-IJGM |
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