Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome
Background An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in ma...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-04-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45464_02168a70fec1630846b8d2ad673d7b02.pdf |
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| author | Zahra Shaaban Mohammad Reza Jafarzadeh Shirazi Mohammad Hossein Nooranizadeh Amin Tamadon Farhad Rahmanifar Somayeh Ahmadloo Amin Ramezani Mohammad Javad Zamiri Iman Razeghian Jahromi Fatemeh Sabet Sarvestani Omid Koohi Hosseinabadi |
| author_facet | Zahra Shaaban Mohammad Reza Jafarzadeh Shirazi Mohammad Hossein Nooranizadeh Amin Tamadon Farhad Rahmanifar Somayeh Ahmadloo Amin Ramezani Mohammad Javad Zamiri Iman Razeghian Jahromi Fatemeh Sabet Sarvestani Omid Koohi Hosseinabadi |
| author_sort | Zahra Shaaban |
| collection | DOAJ |
| description | Background An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. The current study performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus (DMH) after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence of PCOS. Materials and Methods In the experimental study, female nulliparous (n=12) and primiparous (n=12) rats were randomly subdivided into control and PCOS subgroups (n=6). PCOS were induced by 90 days exposure to constant light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control of real-time polymerase chain reaction (PCR) tests were prepared and in the DMH of all rats, the relative mRNA expression of RFRP3 was assessed. Results Histological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of testosterone in the PCOS subgroups were more than the controls (P < 0.05). Furthermore, the relative expression of RFRP3 mRNA in PCOS subgroups was lower than the controls (P < 0.05). Conclusion Constant light model of the PCOS-induced rats decreased the gene expression of RFRP3 in the DMH that suggests the decrease of RFRP3 may reduce its inhibitory effect on GnRH during the PCOS pathogenesis. This effect was stronger in the nulliparous rats than the primiparous. |
| first_indexed | 2024-12-23T06:48:00Z |
| format | Article |
| id | doaj.art-18362ea68c944ac69df7091277934b2e |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-23T06:48:00Z |
| publishDate | 2018-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-18362ea68c944ac69df7091277934b2e2022-12-21T17:56:31ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782018-04-01121435010.22074/ijfs.2018.520645464Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian SyndromeZahra Shaaban0Mohammad Reza Jafarzadeh ShiraziMohammad Hossein Nooranizadeh1Amin Tamadon2Farhad Rahmanifar3Somayeh Ahmadloo4Amin Ramezani5Mohammad Javad Zamiri6Iman Razeghian Jahromi7Fatemeh Sabet Sarvestani8Omid Koohi Hosseinabadi9Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran4Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran;5Institute for Cancer Research, Shiraz University of Medical Sciences, ShirazDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran6Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran7Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, IranBackground An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. The current study performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus (DMH) after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence of PCOS. Materials and Methods In the experimental study, female nulliparous (n=12) and primiparous (n=12) rats were randomly subdivided into control and PCOS subgroups (n=6). PCOS were induced by 90 days exposure to constant light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control of real-time polymerase chain reaction (PCR) tests were prepared and in the DMH of all rats, the relative mRNA expression of RFRP3 was assessed. Results Histological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of testosterone in the PCOS subgroups were more than the controls (P < 0.05). Furthermore, the relative expression of RFRP3 mRNA in PCOS subgroups was lower than the controls (P < 0.05). Conclusion Constant light model of the PCOS-induced rats decreased the gene expression of RFRP3 in the DMH that suggests the decrease of RFRP3 may reduce its inhibitory effect on GnRH during the PCOS pathogenesis. This effect was stronger in the nulliparous rats than the primiparous.http://www.ijfs.ir/article_45464_02168a70fec1630846b8d2ad673d7b02.pdfconstant lightdorsomedial hypothalamic nucleuspolycystic ovary syndromeratsrfamiderelated peptide3 |
| spellingShingle | Zahra Shaaban Mohammad Reza Jafarzadeh Shirazi Mohammad Hossein Nooranizadeh Amin Tamadon Farhad Rahmanifar Somayeh Ahmadloo Amin Ramezani Mohammad Javad Zamiri Iman Razeghian Jahromi Fatemeh Sabet Sarvestani Omid Koohi Hosseinabadi Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome International Journal of Fertility and Sterility constant light dorsomedial hypothalamic nucleus polycystic ovary syndrome rats rfamide relat ed peptide 3 |
| title | Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome |
| title_full | Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome |
| title_fullStr | Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome |
| title_full_unstemmed | Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome |
| title_short | Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome |
| title_sort | decreased expression of arginine phenylalanine amide related peptide 3 gene in dorsomedial hypothalamic nucleus of constant light exposure model of polycystic ovarian syndrome |
| topic | constant light dorsomedial hypothalamic nucleus polycystic ovary syndrome rats rfamide relat ed peptide 3 |
| url | http://www.ijfs.ir/article_45464_02168a70fec1630846b8d2ad673d7b02.pdf |
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