The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome
<i>Lactococcus lactis</i> is a food-grade, and generally recognized as safe, bacterium, which making it ideal for producing plasmid DNA (pDNA) or recombinant proteins for industrial or pharmaceutical applications. The present paper reviews the major findings from <i>L. lactis</i...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/2/267 |
| _version_ | 1827653700592599040 |
|---|---|
| author | Gabriel A. Monteiro Sofia O. D. Duarte |
| author_facet | Gabriel A. Monteiro Sofia O. D. Duarte |
| author_sort | Gabriel A. Monteiro |
| collection | DOAJ |
| description | <i>Lactococcus lactis</i> is a food-grade, and generally recognized as safe, bacterium, which making it ideal for producing plasmid DNA (pDNA) or recombinant proteins for industrial or pharmaceutical applications. The present paper reviews the major findings from <i>L. lactis</i> transcriptome and proteome studies, with an overexpression of native or recombinant proteins. These studies should provide important insights on how to engineer the plasmid vectors and/or the strains in order to achieve high pDNA or recombinant proteins yields, with high quality standards. <i>L. lactis</i> harboring high copy numbers of plasmids for DNA vaccines production showed altered proteome profiles, when compared with a smaller copy number plasmid. For live mucosal vaccination applications, the cell-wall anchored antigens had shown more promising results, when compared with intracellular or secreted antigens. However, previous transcriptome and proteome studies demonstrated that engineering <i>L. lactis</i> to express membrane proteins, mainly with a eukaryotic background, increases the overall cellular burden. Genome engineering strategies could be used to knockout or overexpress the pinpointed genes, so as to increase the profitability of the process. Studies about the effect of protein overexpression on <i>Escherichia coli</i> and <i>Bacillus subtillis</i> transcriptome and proteome are also included. |
| first_indexed | 2024-03-09T21:23:23Z |
| format | Article |
| id | doaj.art-18395b3f4d3f43ef884e5e20479600a6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:23Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-18395b3f4d3f43ef884e5e20479600a62023-11-23T21:13:26ZengMDPI AGMicroorganisms2076-26072022-01-0110226710.3390/microorganisms10020267The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and ProteomeGabriel A. Monteiro0Sofia O. D. Duarte1iBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugaliBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal<i>Lactococcus lactis</i> is a food-grade, and generally recognized as safe, bacterium, which making it ideal for producing plasmid DNA (pDNA) or recombinant proteins for industrial or pharmaceutical applications. The present paper reviews the major findings from <i>L. lactis</i> transcriptome and proteome studies, with an overexpression of native or recombinant proteins. These studies should provide important insights on how to engineer the plasmid vectors and/or the strains in order to achieve high pDNA or recombinant proteins yields, with high quality standards. <i>L. lactis</i> harboring high copy numbers of plasmids for DNA vaccines production showed altered proteome profiles, when compared with a smaller copy number plasmid. For live mucosal vaccination applications, the cell-wall anchored antigens had shown more promising results, when compared with intracellular or secreted antigens. However, previous transcriptome and proteome studies demonstrated that engineering <i>L. lactis</i> to express membrane proteins, mainly with a eukaryotic background, increases the overall cellular burden. Genome engineering strategies could be used to knockout or overexpress the pinpointed genes, so as to increase the profitability of the process. Studies about the effect of protein overexpression on <i>Escherichia coli</i> and <i>Bacillus subtillis</i> transcriptome and proteome are also included.https://www.mdpi.com/2076-2607/10/2/267recombinant protein productionsystems biology<i>Lactococcus lactis</i>cell factorytranscriptomeproteome |
| spellingShingle | Gabriel A. Monteiro Sofia O. D. Duarte The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome Microorganisms recombinant protein production systems biology <i>Lactococcus lactis</i> cell factory transcriptome proteome |
| title | The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome |
| title_full | The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome |
| title_fullStr | The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome |
| title_full_unstemmed | The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome |
| title_short | The Effect of Recombinant Protein Production in <i>Lactococcus lactis</i> Transcriptome and Proteome |
| title_sort | effect of recombinant protein production in i lactococcus lactis i transcriptome and proteome |
| topic | recombinant protein production systems biology <i>Lactococcus lactis</i> cell factory transcriptome proteome |
| url | https://www.mdpi.com/2076-2607/10/2/267 |
| work_keys_str_mv | AT gabrielamonteiro theeffectofrecombinantproteinproductioninilactococcuslactisitranscriptomeandproteome AT sofiaodduarte theeffectofrecombinantproteinproductioninilactococcuslactisitranscriptomeandproteome AT gabrielamonteiro effectofrecombinantproteinproductioninilactococcuslactisitranscriptomeandproteome AT sofiaodduarte effectofrecombinantproteinproductioninilactococcuslactisitranscriptomeandproteome |