Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models
Abstract Background Psoriasis is a chronic inflammatory skin disorder related to dyslipidemia, with decreased high-density lipoprotein (HDL). Various cell types express phospholipid transfer protein (PLTP) as well as cholesteryl ester transfer protein (CETP). Their elevated levels among transgenic (...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12944-022-01684-0 |
| _version_ | 1798038756309073920 |
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| author | Jun Chen Haihua Qi Lijun Liu Yandong Niu Shuping Yu Shucun Qin Lei He |
| author_facet | Jun Chen Haihua Qi Lijun Liu Yandong Niu Shuping Yu Shucun Qin Lei He |
| author_sort | Jun Chen |
| collection | DOAJ |
| description | Abstract Background Psoriasis is a chronic inflammatory skin disorder related to dyslipidemia, with decreased high-density lipoprotein (HDL). Various cell types express phospholipid transfer protein (PLTP) as well as cholesteryl ester transfer protein (CETP). Their elevated levels among transgenic (Tg) mice led to reduced HDL and a higher risk of atherosclerosis (AS). This study examined whether elevated CETP and PLTP could aggravate psoriasis in a psoriasis vulgaris mouse model. Methods The back skins of CETP-Tg, PLTP-Tg, and C57BL/6 male mice, aged six to 8 weeks, were shaved for imiquimod cream (IMQ) (5%) treatment for five consecutive days. The clinical pathological parameters were rated independently using the modified target lesion psoriasis severity score. The skin sections stained with hematoxylin-eosin were scored by the Baker score. Epidermal thickening and differentiation and inflammatory factor infiltration were determined by immunohistochemistry. Inflammatory cytokine levels were measured using quantitative reverse transcription-polymerase chain reaction (RT–PCR) and enzyme-linked immunosorbent assay (ELISA) kits. This work employed SPSS Statistics Version to conduct statistical analyses. Results In this study, CETP-Tg and PLTP-Tg mice had higher clinical and histological scores than wild-type (WT) mice. Immunohistochemistry of the epidermis and dermis revealed a high proportion of proliferating cell nuclear antigen (PCNA) positivity within psoriatic skin lesions of CETP-Tg and PLTP-Tg mice compared with WT mice. Interferon-α (IFN-α), interleukin-1β (IL-1β), IL-6, IL-17A, IL-17F, IL-22, and IL-23p19 mRNA levels increased within CETP-Tg and PLTP-Tg mice compared with WT counterparts. In comparison with WT mice, plasma tumor necrosis factor-α (TNF-α) levels, rather than IL-6 levels, were increased in CETP-Tg and PLTP-Tg mice. Conclusions Elevated CETP and PLTP aggravate psoriasis in a imiquimod-induced mouse model. |
| first_indexed | 2024-04-11T21:44:37Z |
| format | Article |
| id | doaj.art-183e49476bc44e81890f34afb4cf0211 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-11T21:44:37Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-183e49476bc44e81890f34afb4cf02112022-12-22T04:01:27ZengBMCLipids in Health and Disease1476-511X2022-08-0121111010.1186/s12944-022-01684-0Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse modelsJun Chen0Haihua Qi1Lijun Liu2Yandong Niu3Shuping Yu4Shucun Qin5Lei He6Department of Geriatrics, The Affiliated Hospital of Chengde Medical University ChengdeDepartment of Dermatology and Venerology, The Affiliated Hospital of Chengde Medical UniversityDepartment of Dermatology and Venerology, The Affiliated Hospital of Chengde Medical UniversityDepartment of Dermatology and Venerology, The Affiliated Hospital of Chengde Medical UniversityDepartment of Dermatology and Venerology, The Affiliated Hospital of Chengde Medical UniversityInstitute of Atherosclerosis, Shandong First Medical UniversityDepartment of Dermatology and Venerology, The Affiliated Hospital of Chengde Medical UniversityAbstract Background Psoriasis is a chronic inflammatory skin disorder related to dyslipidemia, with decreased high-density lipoprotein (HDL). Various cell types express phospholipid transfer protein (PLTP) as well as cholesteryl ester transfer protein (CETP). Their elevated levels among transgenic (Tg) mice led to reduced HDL and a higher risk of atherosclerosis (AS). This study examined whether elevated CETP and PLTP could aggravate psoriasis in a psoriasis vulgaris mouse model. Methods The back skins of CETP-Tg, PLTP-Tg, and C57BL/6 male mice, aged six to 8 weeks, were shaved for imiquimod cream (IMQ) (5%) treatment for five consecutive days. The clinical pathological parameters were rated independently using the modified target lesion psoriasis severity score. The skin sections stained with hematoxylin-eosin were scored by the Baker score. Epidermal thickening and differentiation and inflammatory factor infiltration were determined by immunohistochemistry. Inflammatory cytokine levels were measured using quantitative reverse transcription-polymerase chain reaction (RT–PCR) and enzyme-linked immunosorbent assay (ELISA) kits. This work employed SPSS Statistics Version to conduct statistical analyses. Results In this study, CETP-Tg and PLTP-Tg mice had higher clinical and histological scores than wild-type (WT) mice. Immunohistochemistry of the epidermis and dermis revealed a high proportion of proliferating cell nuclear antigen (PCNA) positivity within psoriatic skin lesions of CETP-Tg and PLTP-Tg mice compared with WT mice. Interferon-α (IFN-α), interleukin-1β (IL-1β), IL-6, IL-17A, IL-17F, IL-22, and IL-23p19 mRNA levels increased within CETP-Tg and PLTP-Tg mice compared with WT counterparts. In comparison with WT mice, plasma tumor necrosis factor-α (TNF-α) levels, rather than IL-6 levels, were increased in CETP-Tg and PLTP-Tg mice. Conclusions Elevated CETP and PLTP aggravate psoriasis in a imiquimod-induced mouse model.https://doi.org/10.1186/s12944-022-01684-0High-density lipoproteinPsoriasisPhospholipid transfer proteinCholesteryl ester transfer protein |
| spellingShingle | Jun Chen Haihua Qi Lijun Liu Yandong Niu Shuping Yu Shucun Qin Lei He Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models Lipids in Health and Disease High-density lipoprotein Psoriasis Phospholipid transfer protein Cholesteryl ester transfer protein |
| title | Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models |
| title_full | Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models |
| title_fullStr | Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models |
| title_full_unstemmed | Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models |
| title_short | Elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod-induced mouse models |
| title_sort | elevated cholesteryl ester transfer and phospholipid transfer proteins aggravated psoriasis in imiquimod induced mouse models |
| topic | High-density lipoprotein Psoriasis Phospholipid transfer protein Cholesteryl ester transfer protein |
| url | https://doi.org/10.1186/s12944-022-01684-0 |
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