An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress
Various thioredoxin (Trx) proteins have been identified in plants. However, many of the physiological roles played by these proteins remain to be elucidated. We cloned a TRXh-like gene predicted to encode an h-type Trx in tobacco (Nicotiana tabacum) and designated it NtTRXh3, based on the biochemica...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2010-11-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-10-0029 |
| _version_ | 1811291307591598080 |
|---|---|
| author | Lijun Sun Haiying Ren Ruoxue Liu Baoyan Li Tingquan Wu Feng Sun Huimin Liu Xiaomeng Wang Hansong Dong |
| author_facet | Lijun Sun Haiying Ren Ruoxue Liu Baoyan Li Tingquan Wu Feng Sun Huimin Liu Xiaomeng Wang Hansong Dong |
| author_sort | Lijun Sun |
| collection | DOAJ |
| description | Various thioredoxin (Trx) proteins have been identified in plants. However, many of the physiological roles played by these proteins remain to be elucidated. We cloned a TRXh-like gene predicted to encode an h-type Trx in tobacco (Nicotiana tabacum) and designated it NtTRXh3, based on the biochemical activity of the NtTRXh3 protein. Overexpression of NtTRXh3 conferred resistance to Tobacco mosaic virus and Cucumber mosaic virus, both of which showed reduced multiplication and pathogenicity in NtTRXh3-overexpressing plants compared with controls. NtTRXh3 overexpression also enhanced tobacco resistance to oxidative stress induced by paraquat, an herbicide that inhibits the production of reducing equivalents by chloroplasts. The NtTRXh3 protein localized exclusively to chloroplasts in coordination with the maintenance of cellular reducing conditions, which accompanied an elevation in the glutathione/glutathione disulfide couple ratio. NtTRXh3 gene expression and NtTRXh3 protein production were necessary for these defensive responses, because they were all arrested when NtTRXh3 was silenced and the production of NtTRXh3 protein was abrogated. These results suggest that NtTRXh3 is involved in the resistance of tobacco to virus infection and abiotic oxidative stress. |
| first_indexed | 2024-04-13T04:27:24Z |
| format | Article |
| id | doaj.art-183f61d63a244acf94142071fbc4791a |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:24Z |
| publishDate | 2010-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-183f61d63a244acf94142071fbc4791a2022-12-22T03:02:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-11-0123111470148510.1094/MPMI-01-10-0029An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative StressLijun SunHaiying RenRuoxue LiuBaoyan LiTingquan WuFeng SunHuimin LiuXiaomeng WangHansong DongVarious thioredoxin (Trx) proteins have been identified in plants. However, many of the physiological roles played by these proteins remain to be elucidated. We cloned a TRXh-like gene predicted to encode an h-type Trx in tobacco (Nicotiana tabacum) and designated it NtTRXh3, based on the biochemical activity of the NtTRXh3 protein. Overexpression of NtTRXh3 conferred resistance to Tobacco mosaic virus and Cucumber mosaic virus, both of which showed reduced multiplication and pathogenicity in NtTRXh3-overexpressing plants compared with controls. NtTRXh3 overexpression also enhanced tobacco resistance to oxidative stress induced by paraquat, an herbicide that inhibits the production of reducing equivalents by chloroplasts. The NtTRXh3 protein localized exclusively to chloroplasts in coordination with the maintenance of cellular reducing conditions, which accompanied an elevation in the glutathione/glutathione disulfide couple ratio. NtTRXh3 gene expression and NtTRXh3 protein production were necessary for these defensive responses, because they were all arrested when NtTRXh3 was silenced and the production of NtTRXh3 protein was abrogated. These results suggest that NtTRXh3 is involved in the resistance of tobacco to virus infection and abiotic oxidative stress.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-10-0029 |
| spellingShingle | Lijun Sun Haiying Ren Ruoxue Liu Baoyan Li Tingquan Wu Feng Sun Huimin Liu Xiaomeng Wang Hansong Dong An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress Molecular Plant-Microbe Interactions |
| title | An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress |
| title_full | An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress |
| title_fullStr | An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress |
| title_full_unstemmed | An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress |
| title_short | An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress |
| title_sort | h type thioredoxin functions in tobacco defense responses to two species of viruses and an abiotic oxidative stress |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-10-0029 |
| work_keys_str_mv | AT lijunsun anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT haiyingren anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT ruoxueliu anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT baoyanli anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT tingquanwu anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT fengsun anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT huiminliu anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT xiaomengwang anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT hansongdong anhtypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT lijunsun htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT haiyingren htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT ruoxueliu htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT baoyanli htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT tingquanwu htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT fengsun htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT huiminliu htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT xiaomengwang htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress AT hansongdong htypethioredoxinfunctionsintobaccodefenseresponsestotwospeciesofvirusesandanabioticoxidativestress |