Kangxianling formula attenuates renal fibrosis by regulating gut microbiota
Abstract Background Renal fibrosis (RF) produced adverse effect on kidney function. Recently, intestinal dysbiosis is a key regulator that promotes the formation of renal fibrosis. This study will focus on exploring the protective mechanism of Kangxianling Formula (KXL) on renal fibrosis from the pe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-024-01778-8 |
| _version_ | 1827310315081039872 |
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| author | Pengyu Tao Haiyan Liu Guangjian Hou Jianrao Lu Yukun Xu |
| author_facet | Pengyu Tao Haiyan Liu Guangjian Hou Jianrao Lu Yukun Xu |
| author_sort | Pengyu Tao |
| collection | DOAJ |
| description | Abstract Background Renal fibrosis (RF) produced adverse effect on kidney function. Recently, intestinal dysbiosis is a key regulator that promotes the formation of renal fibrosis. This study will focus on exploring the protective mechanism of Kangxianling Formula (KXL) on renal fibrosis from the perspective of intestinal flora. Methods Unilateral Ureteral Obstruction (UUO) was used to construct rats’ model with RF, and receive KXL formula intervention for 1 week. The renal function indicators were measured. Hematoxylin–eosin (HE), Masson and Sirus red staining were employed to detect the pathological changes of renal tissue in each group. The expression of α-SMA, Col-III, TGF-β, FN, ZO-1, and Occuludin was detected by immunofluorescence and immunohistochemistry. Rat feces samples were collected and analyzed for species’ diversity using high-throughput sequencing 16S rRNA. Results Rats in UUO groups displayed poor renal function as well as severe RF. The pro-fibrotic protein expression in renal tissues including α-SMA, Col-III, TGF-β and FN was increased in UUO rats, while ZO-1 and Occuludin -1 expression was downregulated in colon tissues. The above changes were attenuated by KXL treatment. 16S rRNA sequencing results revealed that compared with the sham group, the increased abundance of pathogenic bacteria including Acinetobacter, Enterobacter and Proteobacteria and the decreased abundance of beneficial bacteria including Actinobacteriota, Bifidobacteriales, Prevotellaceae, and Lactobacillus were found in UUO group. After the administration of KXL, the growth of potential pathogenic bacteria was reduced and the abundance of beneficial bacteria was enhanced. Conclusion KXL displays a therapeutical potential in protecting renal function and inhibiting RF, and its mechanism of action may be associated with regulating intestinal microbiota. |
| first_indexed | 2024-04-24T19:57:48Z |
| format | Article |
| id | doaj.art-18465f44a9ca42cc9093bf98bc335bc3 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-24T19:57:48Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-18465f44a9ca42cc9093bf98bc335bc32024-03-24T12:14:14ZengBMCEuropean Journal of Medical Research2047-783X2024-03-0129111210.1186/s40001-024-01778-8Kangxianling formula attenuates renal fibrosis by regulating gut microbiotaPengyu Tao0Haiyan Liu1Guangjian Hou2Jianrao Lu3Yukun Xu4Department of Nephrology, Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Ultrasound, The Affiliated Taian City Central Hospital of Qingdao UniversityShandong University of Traditional Chinese MedicineDepartment of Nephrology, Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Geriatric Medicine, Affiliated Hospital of Shandong University of Traditional Chinese MedicineAbstract Background Renal fibrosis (RF) produced adverse effect on kidney function. Recently, intestinal dysbiosis is a key regulator that promotes the formation of renal fibrosis. This study will focus on exploring the protective mechanism of Kangxianling Formula (KXL) on renal fibrosis from the perspective of intestinal flora. Methods Unilateral Ureteral Obstruction (UUO) was used to construct rats’ model with RF, and receive KXL formula intervention for 1 week. The renal function indicators were measured. Hematoxylin–eosin (HE), Masson and Sirus red staining were employed to detect the pathological changes of renal tissue in each group. The expression of α-SMA, Col-III, TGF-β, FN, ZO-1, and Occuludin was detected by immunofluorescence and immunohistochemistry. Rat feces samples were collected and analyzed for species’ diversity using high-throughput sequencing 16S rRNA. Results Rats in UUO groups displayed poor renal function as well as severe RF. The pro-fibrotic protein expression in renal tissues including α-SMA, Col-III, TGF-β and FN was increased in UUO rats, while ZO-1 and Occuludin -1 expression was downregulated in colon tissues. The above changes were attenuated by KXL treatment. 16S rRNA sequencing results revealed that compared with the sham group, the increased abundance of pathogenic bacteria including Acinetobacter, Enterobacter and Proteobacteria and the decreased abundance of beneficial bacteria including Actinobacteriota, Bifidobacteriales, Prevotellaceae, and Lactobacillus were found in UUO group. After the administration of KXL, the growth of potential pathogenic bacteria was reduced and the abundance of beneficial bacteria was enhanced. Conclusion KXL displays a therapeutical potential in protecting renal function and inhibiting RF, and its mechanism of action may be associated with regulating intestinal microbiota.https://doi.org/10.1186/s40001-024-01778-8Kangxianling formulaRenal fibrosisUUO ratGut |
| spellingShingle | Pengyu Tao Haiyan Liu Guangjian Hou Jianrao Lu Yukun Xu Kangxianling formula attenuates renal fibrosis by regulating gut microbiota European Journal of Medical Research Kangxianling formula Renal fibrosis UUO rat Gut |
| title | Kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| title_full | Kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| title_fullStr | Kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| title_full_unstemmed | Kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| title_short | Kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| title_sort | kangxianling formula attenuates renal fibrosis by regulating gut microbiota |
| topic | Kangxianling formula Renal fibrosis UUO rat Gut |
| url | https://doi.org/10.1186/s40001-024-01778-8 |
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