Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA
About 50 species of cave-obligate Leptodirini (Leiodidae) beetles have been described so far in Romania, most of them populating caves in the Apuseni Mountains (north-western Romania) and the Southern Carpathians. In this contribution, we present the first molecular phylogeographic study of the two...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-02-01
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| Series: | Subterranean Biology |
| Online Access: | https://subtbiol.pensoft.net/article/73524/download/pdf/ |
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| author | Ruxandra Năstase-Bucur Giuliana Allegrucci Valerio Ketmaier Ionuţ Cornel Mirea Oana Teodora Moldovan |
| author_facet | Ruxandra Năstase-Bucur Giuliana Allegrucci Valerio Ketmaier Ionuţ Cornel Mirea Oana Teodora Moldovan |
| author_sort | Ruxandra Năstase-Bucur |
| collection | DOAJ |
| description | About 50 species of cave-obligate Leptodirini (Leiodidae) beetles have been described so far in Romania, most of them populating caves in the Apuseni Mountains (north-western Romania) and the Southern Carpathians. In this contribution, we present the first molecular phylogeographic study of the two troglobiotic Pholeuon species from the Apuseni Mountains. The two species are Pholeuon (s.str.) leptodirum and Pholeuon (Parapholeuon) gracile, endemic to Bihorului Mountains and Pădurea Craiului Mountains, respectively. To examine the genetic divergence within and between the two species we sequenced 571 bp of the mitochondrial COI gene in a total of 145 specimens, 56 specimens of the first species (collected in five caves) and 89 of the second species (collected in eight caves) across their geographic ranges. We found very low genetic variation, four haplotypes in P. leptodirum and seven haplotypes in P. gracile, and a maximum of 0.7% and 0.9% intraspecific divergence, respectively. However, a significant genetic divergence of 6.55% was found between species. The results are consistent with previous definitions of the two species based on morphological characters, while caution should be taken in considering attributions to different subspecies. Our research contributes to the phylogeographic information of troglobitic beetles, providing a solid basis for future comparison with other terrestrial or aquatic cave adapted species. |
| first_indexed | 2024-03-12T09:33:48Z |
| format | Article |
| id | doaj.art-18489aeb1b0d4c21a713f9848e75afca |
| institution | Directory Open Access Journal |
| issn | 1314-2615 |
| language | English |
| last_indexed | 2024-03-12T09:33:48Z |
| publishDate | 2022-02-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Subterranean Biology |
| spelling | doaj.art-18489aeb1b0d4c21a713f9848e75afca2023-09-02T13:49:55ZengPensoft PublishersSubterranean Biology1314-26152022-02-0142617810.3897/subtbiol.42.7352473524Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNARuxandra Năstase-Bucur0Giuliana Allegrucci1Valerio Ketmaier2Ionuţ Cornel Mirea3Oana Teodora Moldovan4Emil Racovitza Institute of SpeleologyUniversity of Rome Tor VergataUnaffiliatedEmil Racovita Institute of SpeleologyRomanian Institute of Science and TechnologyAbout 50 species of cave-obligate Leptodirini (Leiodidae) beetles have been described so far in Romania, most of them populating caves in the Apuseni Mountains (north-western Romania) and the Southern Carpathians. In this contribution, we present the first molecular phylogeographic study of the two troglobiotic Pholeuon species from the Apuseni Mountains. The two species are Pholeuon (s.str.) leptodirum and Pholeuon (Parapholeuon) gracile, endemic to Bihorului Mountains and Pădurea Craiului Mountains, respectively. To examine the genetic divergence within and between the two species we sequenced 571 bp of the mitochondrial COI gene in a total of 145 specimens, 56 specimens of the first species (collected in five caves) and 89 of the second species (collected in eight caves) across their geographic ranges. We found very low genetic variation, four haplotypes in P. leptodirum and seven haplotypes in P. gracile, and a maximum of 0.7% and 0.9% intraspecific divergence, respectively. However, a significant genetic divergence of 6.55% was found between species. The results are consistent with previous definitions of the two species based on morphological characters, while caution should be taken in considering attributions to different subspecies. Our research contributes to the phylogeographic information of troglobitic beetles, providing a solid basis for future comparison with other terrestrial or aquatic cave adapted species.https://subtbiol.pensoft.net/article/73524/download/pdf/ |
| spellingShingle | Ruxandra Năstase-Bucur Giuliana Allegrucci Valerio Ketmaier Ionuţ Cornel Mirea Oana Teodora Moldovan Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA Subterranean Biology |
| title | Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA |
| title_full | Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA |
| title_fullStr | Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA |
| title_full_unstemmed | Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA |
| title_short | Comparative phylogeography of two troglobitic Coleoptera (Leiodidae, Leptodirini) species from Romania based on mitochondrial DNA |
| title_sort | comparative phylogeography of two troglobitic coleoptera leiodidae leptodirini species from romania based on mitochondrial dna |
| url | https://subtbiol.pensoft.net/article/73524/download/pdf/ |
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