Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies
Cellular response to mechanical stimuli is a crucial factor for maintaining cell homeostasis. The interaction between the extracellular matrix and mechanical stress plays a significant role in organizing the cytoskeleton and aligning cells. Tools that apply mechanical forces to cells and tissues, as...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/8/1537 |
| _version_ | 1797583813662998528 |
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| author | Sharda Yadav Pradip Singha Nhat-Khuong Nguyen Chin Hong Ooi Navid Kashaninejad Nam-Trung Nguyen |
| author_facet | Sharda Yadav Pradip Singha Nhat-Khuong Nguyen Chin Hong Ooi Navid Kashaninejad Nam-Trung Nguyen |
| author_sort | Sharda Yadav |
| collection | DOAJ |
| description | Cellular response to mechanical stimuli is a crucial factor for maintaining cell homeostasis. The interaction between the extracellular matrix and mechanical stress plays a significant role in organizing the cytoskeleton and aligning cells. Tools that apply mechanical forces to cells and tissues, as well as those capable of measuring the mechanical properties of biological cells, have greatly contributed to our understanding of fundamental mechanobiology. These tools have been extensively employed to unveil the substantial influence of mechanical cues on the development and progression of various diseases. In this report, we present an economical and high-performance uniaxial cell stretching device. This paper reports the detailed operation concept of the device, experimental design, and characterization. The device was tested with MDA-MB-231 breast cancer cells. The experimental results agree well with previously documented morphological changes resulting from stretching forces on cancer cells. Remarkably, our new device demonstrates comparable cellular changes within 30 min compared with the previous 2 h stretching duration. This third-generation device significantly improved the stretching capabilities compared with its previous counterparts, resulting in a remarkable reduction in stretching time and a substantial increase in overall efficiency. Moreover, the device design incorporates an open-source software interface, facilitating convenient parameter adjustments such as strain, stretching speed, frequency, and duration. Its versatility enables seamless integration with various optical microscopes, thereby yielding novel insights into the realm of mechanobiology. |
| first_indexed | 2024-03-10T23:43:19Z |
| format | Article |
| id | doaj.art-18495ace2f32431f9db02cad534cd93d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T23:43:19Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-18495ace2f32431f9db02cad534cd93d2023-11-19T02:13:07ZengMDPI AGMicromachines2072-666X2023-07-01148153710.3390/mi14081537Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular StudiesSharda Yadav0Pradip Singha1Nhat-Khuong Nguyen2Chin Hong Ooi3Navid Kashaninejad4Nam-Trung Nguyen5Queensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, AustraliaCellular response to mechanical stimuli is a crucial factor for maintaining cell homeostasis. The interaction between the extracellular matrix and mechanical stress plays a significant role in organizing the cytoskeleton and aligning cells. Tools that apply mechanical forces to cells and tissues, as well as those capable of measuring the mechanical properties of biological cells, have greatly contributed to our understanding of fundamental mechanobiology. These tools have been extensively employed to unveil the substantial influence of mechanical cues on the development and progression of various diseases. In this report, we present an economical and high-performance uniaxial cell stretching device. This paper reports the detailed operation concept of the device, experimental design, and characterization. The device was tested with MDA-MB-231 breast cancer cells. The experimental results agree well with previously documented morphological changes resulting from stretching forces on cancer cells. Remarkably, our new device demonstrates comparable cellular changes within 30 min compared with the previous 2 h stretching duration. This third-generation device significantly improved the stretching capabilities compared with its previous counterparts, resulting in a remarkable reduction in stretching time and a substantial increase in overall efficiency. Moreover, the device design incorporates an open-source software interface, facilitating convenient parameter adjustments such as strain, stretching speed, frequency, and duration. Its versatility enables seamless integration with various optical microscopes, thereby yielding novel insights into the realm of mechanobiology.https://www.mdpi.com/2072-666X/14/8/1537mechanobiologycell stretchingbiomedical deviceextracellular matrix |
| spellingShingle | Sharda Yadav Pradip Singha Nhat-Khuong Nguyen Chin Hong Ooi Navid Kashaninejad Nam-Trung Nguyen Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies Micromachines mechanobiology cell stretching biomedical device extracellular matrix |
| title | Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies |
| title_full | Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies |
| title_fullStr | Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies |
| title_full_unstemmed | Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies |
| title_short | Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies |
| title_sort | uniaxial cyclic cell stretching device for accelerating cellular studies |
| topic | mechanobiology cell stretching biomedical device extracellular matrix |
| url | https://www.mdpi.com/2072-666X/14/8/1537 |
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