Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice
(1) Background: In the treatment of ulcerative colitis (UC), accurate delivery and release of anti-inflammatory drugs to the site of inflammation can reduce systemic side effects. (2) Methods: We took advantage of this goal to prepare resveratrol-loaded PLGA nanoparticles (RES-PCAC-NPs) by emulsific...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/8/1884 |
| _version_ | 1797522611265077248 |
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| author | Mengfei Jin Shangyong Li Yanhong Wu Dandan Li Yantao Han |
| author_facet | Mengfei Jin Shangyong Li Yanhong Wu Dandan Li Yantao Han |
| author_sort | Mengfei Jin |
| collection | DOAJ |
| description | (1) Background: In the treatment of ulcerative colitis (UC), accurate delivery and release of anti-inflammatory drugs to the site of inflammation can reduce systemic side effects. (2) Methods: We took advantage of this goal to prepare resveratrol-loaded PLGA nanoparticles (RES-PCAC-NPs) by emulsification solvent volatilization. After layer-by-layer self-assembly technology, we deposited chitosan and alginate to form a three-layer polyelectrolyte film. (3) Results: It can transport nanoparticles through the gastric environment to target inflammation sites and slowly release drugs at a specific pH. The resulting RES-PCAC-NPs have an ideal average diameter (~255 nm), a narrow particle size distribution and a positively charged surface charge (~13.5 mV). The Fourier transform infrared spectroscopy showed that resveratrol was successfully encapsulated into PCAC nanoparticles, and the encapsulation efficiency reached 87.26%. In addition, fluorescence imaging showed that RES-PCAC-NPs with positive charges on the surface can effectively target and accumulate in the inflammation site while continuing to penetrate downward to promote mucosal healing. Importantly, oral RES-PCAC-NPs treatment in DSS-induced mice was superior to other results in significantly improved inflammatory markers of UC. (4) Conclusions: Our results strongly prove that RES-PCAC-NPs can target the inflamed colon for maximum efficacy, and this oral pharmaceutical formulation can represent a promising formulation in the treatment of UC. |
| first_indexed | 2024-03-10T08:31:52Z |
| format | Article |
| id | doaj.art-184b7624b6194ccdafabc59d78362320 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T08:31:52Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-184b7624b6194ccdafabc59d783623202023-11-22T08:57:12ZengMDPI AGNanomaterials2079-49912021-07-01118188410.3390/nano11081884Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in MiceMengfei Jin0Shangyong Li1Yanhong Wu2Dandan Li3Yantao Han4School of Basic Medicine, Qingdao University, Ningxia Road 308, Qingdao 266071, ChinaSchool of Basic Medicine, Qingdao University, Ningxia Road 308, Qingdao 266071, ChinaSchool of Basic Medicine, Qingdao University, Ningxia Road 308, Qingdao 266071, ChinaSchool of Basic Medicine, Qingdao University, Ningxia Road 308, Qingdao 266071, ChinaSchool of Basic Medicine, Qingdao University, Ningxia Road 308, Qingdao 266071, China(1) Background: In the treatment of ulcerative colitis (UC), accurate delivery and release of anti-inflammatory drugs to the site of inflammation can reduce systemic side effects. (2) Methods: We took advantage of this goal to prepare resveratrol-loaded PLGA nanoparticles (RES-PCAC-NPs) by emulsification solvent volatilization. After layer-by-layer self-assembly technology, we deposited chitosan and alginate to form a three-layer polyelectrolyte film. (3) Results: It can transport nanoparticles through the gastric environment to target inflammation sites and slowly release drugs at a specific pH. The resulting RES-PCAC-NPs have an ideal average diameter (~255 nm), a narrow particle size distribution and a positively charged surface charge (~13.5 mV). The Fourier transform infrared spectroscopy showed that resveratrol was successfully encapsulated into PCAC nanoparticles, and the encapsulation efficiency reached 87.26%. In addition, fluorescence imaging showed that RES-PCAC-NPs with positive charges on the surface can effectively target and accumulate in the inflammation site while continuing to penetrate downward to promote mucosal healing. Importantly, oral RES-PCAC-NPs treatment in DSS-induced mice was superior to other results in significantly improved inflammatory markers of UC. (4) Conclusions: Our results strongly prove that RES-PCAC-NPs can target the inflamed colon for maximum efficacy, and this oral pharmaceutical formulation can represent a promising formulation in the treatment of UC.https://www.mdpi.com/2079-4991/11/8/1884resveratrolnanoparticleschitosanalginatedeliveryUC |
| spellingShingle | Mengfei Jin Shangyong Li Yanhong Wu Dandan Li Yantao Han Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice Nanomaterials resveratrol nanoparticles chitosan alginate delivery UC |
| title | Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice |
| title_full | Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice |
| title_fullStr | Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice |
| title_full_unstemmed | Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice |
| title_short | Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice |
| title_sort | construction of chitosan alginate nano drug delivery system for improving dextran sodium sulfate induced colitis in mice |
| topic | resveratrol nanoparticles chitosan alginate delivery UC |
| url | https://www.mdpi.com/2079-4991/11/8/1884 |
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