Detection of anti-Giardia lamblia serum antibody among children of day care centers
OBJETIVES: To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas. METHODS: Three separate stool specimens and filter paper blood samples were collecte...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2002-02-01
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| Series: | Revista de Saúde Pública |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-89102002000100010&lng=en&tlng=en |
| _version_ | 1818996129635762176 |
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| author | Semíramis Guimarães Maria Inês L Sogayar |
| author_facet | Semíramis Guimarães Maria Inês L Sogayar |
| author_sort | Semíramis Guimarães |
| collection | DOAJ |
| description | OBJETIVES: To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas. METHODS: Three separate stool specimens and filter paper blood samples were collected from 147 children ranging from 0 to 6 years old. Each stool sample was processed using spontaneous sedimentation and zinc sulfate flotation methods. Blood samples were tested by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) for Giardia IgG. RESULTS AND CONCLUSIONS: Of 147 individuals tested, 93 (63.3%) showed Giardia cysts in their feces. Using IIF and ELISA, serum antibodies were detected in 93 (63.3%) and 100 (68%) samples , respectively. Sensitivity of IIF and ELISA was 82% and 72%, respectively. However, ELISA revealed to be less specific (39%) than IIF (70%). IIF also showed a higher concordance with microscopic examination than ELISA. |
| first_indexed | 2024-12-20T21:24:50Z |
| format | Article |
| id | doaj.art-184c0caf917a486ba45d8b0f3af36dc5 |
| institution | Directory Open Access Journal |
| issn | 1518-8787 |
| language | English |
| last_indexed | 2024-12-20T21:24:50Z |
| publishDate | 2002-02-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Revista de Saúde Pública |
| spelling | doaj.art-184c0caf917a486ba45d8b0f3af36dc52022-12-21T19:26:11ZengUniversidade de São PauloRevista de Saúde Pública1518-87872002-02-01361636810.1590/s0034-89102002000100010S0034-89102002000100010Detection of anti-Giardia lamblia serum antibody among children of day care centersSemíramis Guimarães0Maria Inês L Sogayar1Universidade Estadual PaulistaUniversidade Estadual PaulistaOBJETIVES: To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas. METHODS: Three separate stool specimens and filter paper blood samples were collected from 147 children ranging from 0 to 6 years old. Each stool sample was processed using spontaneous sedimentation and zinc sulfate flotation methods. Blood samples were tested by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) for Giardia IgG. RESULTS AND CONCLUSIONS: Of 147 individuals tested, 93 (63.3%) showed Giardia cysts in their feces. Using IIF and ELISA, serum antibodies were detected in 93 (63.3%) and 100 (68%) samples , respectively. Sensitivity of IIF and ELISA was 82% and 72%, respectively. However, ELISA revealed to be less specific (39%) than IIF (70%). IIF also showed a higher concordance with microscopic examination than ELISA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-89102002000100010&lng=en&tlng=enGiardia lambliaAnticorpos antiprotozoáriosTestes sorológicosElisaTécnica de imunofluorescência indiretaFezesCreches |
| spellingShingle | Semíramis Guimarães Maria Inês L Sogayar Detection of anti-Giardia lamblia serum antibody among children of day care centers Revista de Saúde Pública Giardia lamblia Anticorpos antiprotozoários Testes sorológicos Elisa Técnica de imunofluorescência indireta Fezes Creches |
| title | Detection of anti-Giardia lamblia serum antibody among children of day care centers |
| title_full | Detection of anti-Giardia lamblia serum antibody among children of day care centers |
| title_fullStr | Detection of anti-Giardia lamblia serum antibody among children of day care centers |
| title_full_unstemmed | Detection of anti-Giardia lamblia serum antibody among children of day care centers |
| title_short | Detection of anti-Giardia lamblia serum antibody among children of day care centers |
| title_sort | detection of anti giardia lamblia serum antibody among children of day care centers |
| topic | Giardia lamblia Anticorpos antiprotozoários Testes sorológicos Elisa Técnica de imunofluorescência indireta Fezes Creches |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-89102002000100010&lng=en&tlng=en |
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