Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources
The objective of this study was to investigate the impacts of Bacillus subtilis (BS), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [SBM] and cowpea seeds [CWP]), on growth performance (GP), carcass traits, bone mineralization, and microflora p...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120306222 |
| _version_ | 1818989064999665664 |
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| author | G. Ciurescu M. Dumitru A. Gheorghe A.E. Untea R. Drăghici |
| author_facet | G. Ciurescu M. Dumitru A. Gheorghe A.E. Untea R. Drăghici |
| author_sort | G. Ciurescu |
| collection | DOAJ |
| description | The objective of this study was to investigate the impacts of Bacillus subtilis (BS), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [SBM] and cowpea seeds [CWP]), on growth performance (GP), carcass traits, bone mineralization, and microflora population (0 to 42 d age). The SBM and CWP starter, grower, and finisher diets were tested in the presence or absence of BS (5.0 × 1011 CFU spores g−1 feed) in a 2 × 2 factorial arrangement of treatments in a completely randomized design. Broilers were randomly assigned to 4 dietary treatments with 6 replicate pens per treatment (20 chicks per pen). The results showed that broilers fed CWP had comparable GP (body weight gain, feed intake, and feed conversion ratio) to the birds fed the SBM diet. Carcass, breast and legs' yield, organ size (i.e., gizzard, liver, pancreas, small intestine, cecum), and bone development were not affected by the protein source. The addition of BS in both types of diet improved BWG (P < 0.001) and feed efficiency, especially in the grower and finisher period (P = 0.047; P = 0.043, respectively). In addition, BS significantly decreased abdominal fat (P = 0.026) and cecum weight (P = 0.034) and increased tibia bone P concentration (P = 0.015). Furthermore, BS decrease cecal pH (P = 0.010) and reduced Escherichia coli and Staphylococcus spp. from cecum and excreta broilers (P < 0.001; P < 0.0001, respectively). It is concluded that the BS significantly improved the GP of broilers and can beneficially affect the gut and excreta bacterial community in both SBM and CWP diets. |
| first_indexed | 2024-12-20T19:32:33Z |
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| id | doaj.art-184e4d21e2b643aea8d5219bc1fdb8bf |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-20T19:32:33Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-184e4d21e2b643aea8d5219bc1fdb8bf2022-12-21T19:28:44ZengElsevierPoultry Science0032-57912020-11-01991159605971Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sourcesG. Ciurescu0M. Dumitru1A. Gheorghe2A.E. Untea3R. Drăghici4National Research-Development Institute for Biology and Animal Nutrition Calea București, Ilfov, RomaniaNational Research-Development Institute for Biology and Animal Nutrition Calea București, Ilfov, Romania; Department of Biotechnology, Doctoral School, University of Agronomic Science and Veterinary Medicine, Bucharest, RomaniaNational Research-Development Institute for Biology and Animal Nutrition Calea București, Ilfov, Romania; Corresponding author:National Research-Development Institute for Biology and Animal Nutrition Calea București, Ilfov, RomaniaResearch-Development Station for Plant Culture on Sands, Dăbuleni, Dolj, RomaniaThe objective of this study was to investigate the impacts of Bacillus subtilis (BS), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [SBM] and cowpea seeds [CWP]), on growth performance (GP), carcass traits, bone mineralization, and microflora population (0 to 42 d age). The SBM and CWP starter, grower, and finisher diets were tested in the presence or absence of BS (5.0 × 1011 CFU spores g−1 feed) in a 2 × 2 factorial arrangement of treatments in a completely randomized design. Broilers were randomly assigned to 4 dietary treatments with 6 replicate pens per treatment (20 chicks per pen). The results showed that broilers fed CWP had comparable GP (body weight gain, feed intake, and feed conversion ratio) to the birds fed the SBM diet. Carcass, breast and legs' yield, organ size (i.e., gizzard, liver, pancreas, small intestine, cecum), and bone development were not affected by the protein source. The addition of BS in both types of diet improved BWG (P < 0.001) and feed efficiency, especially in the grower and finisher period (P = 0.047; P = 0.043, respectively). In addition, BS significantly decreased abdominal fat (P = 0.026) and cecum weight (P = 0.034) and increased tibia bone P concentration (P = 0.015). Furthermore, BS decrease cecal pH (P = 0.010) and reduced Escherichia coli and Staphylococcus spp. from cecum and excreta broilers (P < 0.001; P < 0.0001, respectively). It is concluded that the BS significantly improved the GP of broilers and can beneficially affect the gut and excreta bacterial community in both SBM and CWP diets.http://www.sciencedirect.com/science/article/pii/S0032579120306222cowpeaprobioticbroiler performancegut microflora |
| spellingShingle | G. Ciurescu M. Dumitru A. Gheorghe A.E. Untea R. Drăghici Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources Poultry Science cowpea probiotic broiler performance gut microflora |
| title | Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources |
| title_full | Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources |
| title_fullStr | Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources |
| title_full_unstemmed | Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources |
| title_short | Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources |
| title_sort | effect of bacillus subtilis on growth performance bone mineralization and bacterial population of broilers fed with different protein sources |
| topic | cowpea probiotic broiler performance gut microflora |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120306222 |
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