Accelerated staining technique using kitchen microwave oven

Introduction: Histopathological diagnosis of specimens is greatly dependent on good sample preparation and staining. Both of these processes is governed by diffusion of fluids and dyes in and out of the tissue, which is the key to staining. Diffusion of fluids can be accelerated by the application o...

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Main Authors: Archana Mukunda, T V Narayan, Balasundhari Shreedhar, R Shashidhara, Leeky Mohanty, Sadhana Shenoy
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:Indian Journal of Pathology and Microbiology
Subjects:
Online Access:http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=316;epage=322;aulast=Mukunda
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author Archana Mukunda
T V Narayan
Balasundhari Shreedhar
R Shashidhara
Leeky Mohanty
Sadhana Shenoy
author_facet Archana Mukunda
T V Narayan
Balasundhari Shreedhar
R Shashidhara
Leeky Mohanty
Sadhana Shenoy
author_sort Archana Mukunda
collection DOAJ
description Introduction: Histopathological diagnosis of specimens is greatly dependent on good sample preparation and staining. Both of these processes is governed by diffusion of fluids and dyes in and out of the tissue, which is the key to staining. Diffusion of fluids can be accelerated by the application of heat that reduces the time of staining from hours to the minute. We modified an inexpensive model of kitchen microwave oven for staining. This study is an attempt to compare the reliability of this modified technique against the tested technique of routine staining so as to establish the kitchen microwave oven as a valuable diagnostic tool. Materials and Methods: Sixty different tissue blocks were used to prepare 20 pairs of slides for 4 different stains namely hematoxylin and eosin, Van Gieson′s, 0.1% toluidine blue and periodic acid-Schiff. From each tissue block, two bits of tissues were mounted on two different slides. One slide was stained routinely, and the other stained inside a microwave. A pathologist evaluated the stained slides and the results so obtained were analyzed statistically. Results: Microwave staining considerably cut down the staining time from hours to seconds. Microwave staining showed no loss of cellular and nuclear details, uniform-staining characteristics and was of excellent quality. Interpretation and Conclusion: The cellular details, nuclear details and staining characteristics of microwave stained tissues were better than or equal to the routine stained tissue. The overall quality of microwave-stained sections was found to be better than the routine stained tissue in majority of cases.
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spelling doaj.art-185043acfbc54cb5af432abbc0e6b6e42022-12-22T02:36:29ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292015-01-0158331632210.4103/0377-4929.162863Accelerated staining technique using kitchen microwave ovenArchana MukundaT V NarayanBalasundhari ShreedharR ShashidharaLeeky MohantySadhana ShenoyIntroduction: Histopathological diagnosis of specimens is greatly dependent on good sample preparation and staining. Both of these processes is governed by diffusion of fluids and dyes in and out of the tissue, which is the key to staining. Diffusion of fluids can be accelerated by the application of heat that reduces the time of staining from hours to the minute. We modified an inexpensive model of kitchen microwave oven for staining. This study is an attempt to compare the reliability of this modified technique against the tested technique of routine staining so as to establish the kitchen microwave oven as a valuable diagnostic tool. Materials and Methods: Sixty different tissue blocks were used to prepare 20 pairs of slides for 4 different stains namely hematoxylin and eosin, Van Gieson′s, 0.1% toluidine blue and periodic acid-Schiff. From each tissue block, two bits of tissues were mounted on two different slides. One slide was stained routinely, and the other stained inside a microwave. A pathologist evaluated the stained slides and the results so obtained were analyzed statistically. Results: Microwave staining considerably cut down the staining time from hours to seconds. Microwave staining showed no loss of cellular and nuclear details, uniform-staining characteristics and was of excellent quality. Interpretation and Conclusion: The cellular details, nuclear details and staining characteristics of microwave stained tissues were better than or equal to the routine stained tissue. The overall quality of microwave-stained sections was found to be better than the routine stained tissue in majority of cases.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=316;epage=322;aulast=MukundaKitchen microwave ovenmicrowave stainingroutine staining
spellingShingle Archana Mukunda
T V Narayan
Balasundhari Shreedhar
R Shashidhara
Leeky Mohanty
Sadhana Shenoy
Accelerated staining technique using kitchen microwave oven
Indian Journal of Pathology and Microbiology
Kitchen microwave oven
microwave staining
routine staining
title Accelerated staining technique using kitchen microwave oven
title_full Accelerated staining technique using kitchen microwave oven
title_fullStr Accelerated staining technique using kitchen microwave oven
title_full_unstemmed Accelerated staining technique using kitchen microwave oven
title_short Accelerated staining technique using kitchen microwave oven
title_sort accelerated staining technique using kitchen microwave oven
topic Kitchen microwave oven
microwave staining
routine staining
url http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=316;epage=322;aulast=Mukunda
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AT balasundharishreedhar acceleratedstainingtechniqueusingkitchenmicrowaveoven
AT rshashidhara acceleratedstainingtechniqueusingkitchenmicrowaveoven
AT leekymohanty acceleratedstainingtechniqueusingkitchenmicrowaveoven
AT sadhanashenoy acceleratedstainingtechniqueusingkitchenmicrowaveoven