Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery
The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evalua...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-04-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4923/5/2/246 |
| _version_ | 1818039765481553920 |
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| author | Torben Moos Jacek Lichota Jesper B. Jensen Jens C. Laursen Kristian G. du Jardin Thomas Linemann Louiza B. Thomsen |
| author_facet | Torben Moos Jacek Lichota Jesper B. Jensen Jens C. Laursen Kristian G. du Jardin Thomas Linemann Louiza B. Thomsen |
| author_sort | Torben Moos |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro. |
| first_indexed | 2024-12-10T08:03:50Z |
| format | Article |
| id | doaj.art-185473141c5747abbf22ac097866826a |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-12-10T08:03:50Z |
| publishDate | 2013-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-185473141c5747abbf22ac097866826a2022-12-22T01:56:43ZengMDPI AGPharmaceutics1999-49232013-04-015224626010.3390/pharmaceutics5020246Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug DeliveryTorben MoosJacek LichotaJesper B. JensenJens C. LaursenKristian G. du JardinThomas LinemannLouiza B. ThomsenThe aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro.http://www.mdpi.com/1999-4923/5/2/246blood-brain barrierendotheliummagnetofectionmagnetic fieldnanoparticletransfection |
| spellingShingle | Torben Moos Jacek Lichota Jesper B. Jensen Jens C. Laursen Kristian G. du Jardin Thomas Linemann Louiza B. Thomsen Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery Pharmaceutics blood-brain barrier endothelium magnetofection magnetic field nanoparticle transfection |
| title | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
| title_full | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
| title_fullStr | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
| title_full_unstemmed | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
| title_short | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
| title_sort | development of a novel lipophilic magnetic nanoparticle for in vivo drug delivery |
| topic | blood-brain barrier endothelium magnetofection magnetic field nanoparticle transfection |
| url | http://www.mdpi.com/1999-4923/5/2/246 |
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