Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs
Abstract In sheep, nutrition during the prepubertal stage is essential for growth performance and mammary gland development. However, the potential effects of nutrient restriction in a prepuberal stage over the progeny still need to be better understood. Here, the intergenerational effect of materna...
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31546-3 |
| _version_ | 1797865022605492224 |
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| author | Pablo A. S. Fonseca Aroa Suárez-Vega Rocio Pelayo Hector Marina María Alonso-García Beatriz Gutiérrez-Gil Juan-José Arranz |
| author_facet | Pablo A. S. Fonseca Aroa Suárez-Vega Rocio Pelayo Hector Marina María Alonso-García Beatriz Gutiérrez-Gil Juan-José Arranz |
| author_sort | Pablo A. S. Fonseca |
| collection | DOAJ |
| description | Abstract In sheep, nutrition during the prepubertal stage is essential for growth performance and mammary gland development. However, the potential effects of nutrient restriction in a prepuberal stage over the progeny still need to be better understood. Here, the intergenerational effect of maternal protein restriction at prepubertal age (2 months of age) on methylation patterns was evaluated in the perirenal fat of Assaf suckling lambs. In total, 17 lambs from ewes subjected to dietary protein restriction (NPR group, 44% less protein) and 17 lambs from control ewes (C group) were analyzed. These lambs were ranked based on their carcass proportion of perirenal and cavitary fat and classified into HighPCF and LowPCF groups. The perirenal tissue from 4 NPR-LowPCF, 4 NPR-HighPCF, 4 C-LowPCF, and 4 C-HighPCF lambs was subjected to whole-genome bisulfite sequencing and differentially methylated regions (DMRs) were identified. Among other relevant processes, these DMRs were mapped in genes responsible for regulating the transition of brown to white adipose tissue and nonshivering thermoregulation, which might be associated with better adaptation/survival of lambs in the perinatal stage. The current study provides important biological insights about the intergenerational effect on the methylation pattern of an NPR in replacement ewes. |
| first_indexed | 2024-04-09T23:01:23Z |
| format | Article |
| id | doaj.art-185ae9439e4e46fd8db30cb10c907e6e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T23:01:23Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-185ae9439e4e46fd8db30cb10c907e6e2023-03-22T10:54:33ZengNature PortfolioScientific Reports2045-23222023-03-0113111510.1038/s41598-023-31546-3Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambsPablo A. S. Fonseca0Aroa Suárez-Vega1Rocio Pelayo2Hector Marina3María Alonso-García4Beatriz Gutiérrez-Gil5Juan-José Arranz6Departamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónAbstract In sheep, nutrition during the prepubertal stage is essential for growth performance and mammary gland development. However, the potential effects of nutrient restriction in a prepuberal stage over the progeny still need to be better understood. Here, the intergenerational effect of maternal protein restriction at prepubertal age (2 months of age) on methylation patterns was evaluated in the perirenal fat of Assaf suckling lambs. In total, 17 lambs from ewes subjected to dietary protein restriction (NPR group, 44% less protein) and 17 lambs from control ewes (C group) were analyzed. These lambs were ranked based on their carcass proportion of perirenal and cavitary fat and classified into HighPCF and LowPCF groups. The perirenal tissue from 4 NPR-LowPCF, 4 NPR-HighPCF, 4 C-LowPCF, and 4 C-HighPCF lambs was subjected to whole-genome bisulfite sequencing and differentially methylated regions (DMRs) were identified. Among other relevant processes, these DMRs were mapped in genes responsible for regulating the transition of brown to white adipose tissue and nonshivering thermoregulation, which might be associated with better adaptation/survival of lambs in the perinatal stage. The current study provides important biological insights about the intergenerational effect on the methylation pattern of an NPR in replacement ewes.https://doi.org/10.1038/s41598-023-31546-3 |
| spellingShingle | Pablo A. S. Fonseca Aroa Suárez-Vega Rocio Pelayo Hector Marina María Alonso-García Beatriz Gutiérrez-Gil Juan-José Arranz Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs Scientific Reports |
| title | Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| title_full | Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| title_fullStr | Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| title_full_unstemmed | Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| title_short | Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| title_sort | intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs |
| url | https://doi.org/10.1038/s41598-023-31546-3 |
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