Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing
Plant endophyte and epiphyte communities cooperatively interact with their host plants and play crucial roles in sustaining plant fitness. In Korea, a variety of studies have been conducted to elucidate the reasons for the declining population of the endangered Korean fir (<i>Abies koreana<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/13/11/1932 |
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| author | Bae Young Choi Suhyeon Lee Jaewook Kim Hyeonseon Park Joon-Hyeok Kim Minji Kim Soo-Je Park Ki-Tae Kim Hojin Ryu Donghwan Shim |
| author_facet | Bae Young Choi Suhyeon Lee Jaewook Kim Hyeonseon Park Joon-Hyeok Kim Minji Kim Soo-Je Park Ki-Tae Kim Hojin Ryu Donghwan Shim |
| author_sort | Bae Young Choi |
| collection | DOAJ |
| description | Plant endophyte and epiphyte communities cooperatively interact with their host plants and play crucial roles in sustaining plant fitness. In Korea, a variety of studies have been conducted to elucidate the reasons for the declining population of the endangered Korean fir (<i>Abies koreana</i>), but the relationship between microbiota and the healthy condition of trees remains unclear. Here, we conducted bacterial 16S rRNA gene and fungal ITS sequence analyses to dissect the composition of endophytic and epiphytic microbiota in both live and dead trees located in the same Mt. Jiri habitat. In the live trees, the bacterial class Armatimonadia and the lichenized fungi groups were significantly dominant, whereas many bacterial and fungal taxa mainly found in rotten wood were enriched in the dead trees. Functional prediction of the microbial communities in live trees suggested the possibility that bacterial endophytes and epiphytes play a role in inorganic nutrient metabolism and fungal endophytes and epiphytes produce biologically active secondary metabolites, thereby contributing to the healthy condition of Korean fir trees. The ecological function of endophytes and epiphytes in dead trees was predicted to be involved in the decomposition of wood for nutrient recycling. Our analyses revealed a distinct difference in microbial communities depending on the health condition of Korean fir trees. The results from this study would be useful for understanding the ecological function of endophytic and epiphytic microorganisms to conserve and manage this endangered species from ecologically vulnerable environments. |
| first_indexed | 2024-03-09T18:19:18Z |
| format | Article |
| id | doaj.art-185bd1eb5d61403ba715a83094b954cc |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T18:19:18Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-185bd1eb5d61403ba715a83094b954cc2023-11-24T08:24:17ZengMDPI AGForests1999-49072022-11-011311193210.3390/f13111932Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic SequencingBae Young Choi0Suhyeon Lee1Jaewook Kim2Hyeonseon Park3Joon-Hyeok Kim4Minji Kim5Soo-Je Park6Ki-Tae Kim7Hojin Ryu8Donghwan Shim9Department of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaDivision of Forest Bio-Information, National Institute of Forest Science, Suwon 16631, Republic of KoreaDepartment of Biology, Jeju National University, Jeju 63243, Republic of KoreaDepartment of Biology, Jeju National University, Jeju 63243, Republic of KoreaDepartment of Agricultural Life Science, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Biology, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaPlant endophyte and epiphyte communities cooperatively interact with their host plants and play crucial roles in sustaining plant fitness. In Korea, a variety of studies have been conducted to elucidate the reasons for the declining population of the endangered Korean fir (<i>Abies koreana</i>), but the relationship between microbiota and the healthy condition of trees remains unclear. Here, we conducted bacterial 16S rRNA gene and fungal ITS sequence analyses to dissect the composition of endophytic and epiphytic microbiota in both live and dead trees located in the same Mt. Jiri habitat. In the live trees, the bacterial class Armatimonadia and the lichenized fungi groups were significantly dominant, whereas many bacterial and fungal taxa mainly found in rotten wood were enriched in the dead trees. Functional prediction of the microbial communities in live trees suggested the possibility that bacterial endophytes and epiphytes play a role in inorganic nutrient metabolism and fungal endophytes and epiphytes produce biologically active secondary metabolites, thereby contributing to the healthy condition of Korean fir trees. The ecological function of endophytes and epiphytes in dead trees was predicted to be involved in the decomposition of wood for nutrient recycling. Our analyses revealed a distinct difference in microbial communities depending on the health condition of Korean fir trees. The results from this study would be useful for understanding the ecological function of endophytic and epiphytic microorganisms to conserve and manage this endangered species from ecologically vulnerable environments.https://www.mdpi.com/1999-4907/13/11/1932community diversity<i>Abies koreana</i>plant endophyteplant epiphytemetagenomeendangered species |
| spellingShingle | Bae Young Choi Suhyeon Lee Jaewook Kim Hyeonseon Park Joon-Hyeok Kim Minji Kim Soo-Je Park Ki-Tae Kim Hojin Ryu Donghwan Shim Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing Forests community diversity <i>Abies koreana</i> plant endophyte plant epiphyte metagenome endangered species |
| title | Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing |
| title_full | Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing |
| title_fullStr | Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing |
| title_full_unstemmed | Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing |
| title_short | Comparison of Endophytic and Epiphytic Microbial Communities in Surviving and Dead Korean Fir (<i>Abies koreana</i>) Using Metagenomic Sequencing |
| title_sort | comparison of endophytic and epiphytic microbial communities in surviving and dead korean fir i abies koreana i using metagenomic sequencing |
| topic | community diversity <i>Abies koreana</i> plant endophyte plant epiphyte metagenome endangered species |
| url | https://www.mdpi.com/1999-4907/13/11/1932 |
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