Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity
<i>Viscum album</i> L. (<i>Santalaceae</i>), also known as European mistletoe, is a semi-parasitic plant that grows on different host trees. Our group recently demonstrated the antitumoral activity of ethanolic <i>V. album</i> extracts in vitro, depending on the d...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/1/37 |
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| author | João V. D. C. Batista Ana Paula S. Matos Adriana P. Oliveria Eduardo Ricci Júnior Zaida M. Freitas Catarina A. Oliveira Helena K. Toma Marcia A. M. Capella Leandro M. Rocha Ulrike Weissenstein Stephan Baumgartner Carla Holandino |
| author_facet | João V. D. C. Batista Ana Paula S. Matos Adriana P. Oliveria Eduardo Ricci Júnior Zaida M. Freitas Catarina A. Oliveira Helena K. Toma Marcia A. M. Capella Leandro M. Rocha Ulrike Weissenstein Stephan Baumgartner Carla Holandino |
| author_sort | João V. D. C. Batista |
| collection | DOAJ |
| description | <i>Viscum album</i> L. (<i>Santalaceae</i>), also known as European mistletoe, is a semi-parasitic plant that grows on different host trees. Our group recently demonstrated the antitumoral activity of ethanolic <i>V. album</i> extracts in vitro, depending on the dose and the host tree, <i>V. album</i> ssp <i>abietis</i> from <i>Abies alba</i> being the most active extract. The goal of this work focused on the development of a new topical formulation containing <i>V. album</i> extracts, evaluation of in vitro toxicity and ex vivo skin permeation assays. The Poloxamer 407 hydrogel containing 5% of dry (VA_DEH) or aqueous (VA_AEH) extract presented dermal compatible pH and microbiological stability for 180 days. The hydrogels flow curve presented a non-linear relation, characteristic of non-Newtonian fluids, and the mean viscosity for the VA_DEH and VA_AEH was 372.5 ± 7.78 and 331.0 ± 2.83 Pa.s, respectively, being statistically different (Welch’s <i>t</i> test; <i>p</i> < 0.01). Additionally, WST-1 in vitro assays revealed a dose-dependent toxicity for both formulations and VA_DEH presented a higher activity than the VA_AEH. The promising cytotoxic potential of VA_DEH lead to the ex vivo skin permeation assay with 2.73 ± 0.19 µg/cm<sup>2</sup> of chlorogenic acid, which permeated at 8 h, showing a transdermal potential. These in vitro results support the idea that VA_DEH is a novel promising candidate for mistletoe therapy. Therefore, further in vivo and pre-clinical experiments should be performed to evaluate the safety and efficacy of this new dermic delivery system. |
| first_indexed | 2024-03-10T00:43:49Z |
| format | Article |
| id | doaj.art-185f5f467c4c4b21852fa2c7c83c4afc |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T00:43:49Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-185f5f467c4c4b21852fa2c7c83c4afc2023-11-23T15:02:51ZengMDPI AGPharmaceutics1999-49232021-12-011413710.3390/pharmaceutics14010037Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity ActivityJoão V. D. C. Batista0Ana Paula S. Matos1Adriana P. Oliveria2Eduardo Ricci Júnior3Zaida M. Freitas4Catarina A. Oliveira5Helena K. Toma6Marcia A. M. Capella7Leandro M. Rocha8Ulrike Weissenstein9Stephan Baumgartner10Carla Holandino11Laboratório Multidisciplinar em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório de Desenvolvimento Galênico, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório Multidisciplinar em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório de Desenvolvimento Galênico, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório de Desenvolvimento Galênico, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório de Alimentos, Instituto Federal de Educacão, Ciência e Tecnologia do Rio de Janeiro, Rio de Janeiro 20270-021, RJ, BrazilLaboratório de Diagnóstico Molecular e Hematologia, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaRBio Carlos Chagas Filho, Biophysics Institute, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niteroi 24241-000, RJ, BrazilHiscia Institute, Society for Cancer Research, 4144 Arlesheim, SwitzerlandHiscia Institute, Society for Cancer Research, 4144 Arlesheim, SwitzerlandLaboratório Multidisciplinar em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil<i>Viscum album</i> L. (<i>Santalaceae</i>), also known as European mistletoe, is a semi-parasitic plant that grows on different host trees. Our group recently demonstrated the antitumoral activity of ethanolic <i>V. album</i> extracts in vitro, depending on the dose and the host tree, <i>V. album</i> ssp <i>abietis</i> from <i>Abies alba</i> being the most active extract. The goal of this work focused on the development of a new topical formulation containing <i>V. album</i> extracts, evaluation of in vitro toxicity and ex vivo skin permeation assays. The Poloxamer 407 hydrogel containing 5% of dry (VA_DEH) or aqueous (VA_AEH) extract presented dermal compatible pH and microbiological stability for 180 days. The hydrogels flow curve presented a non-linear relation, characteristic of non-Newtonian fluids, and the mean viscosity for the VA_DEH and VA_AEH was 372.5 ± 7.78 and 331.0 ± 2.83 Pa.s, respectively, being statistically different (Welch’s <i>t</i> test; <i>p</i> < 0.01). Additionally, WST-1 in vitro assays revealed a dose-dependent toxicity for both formulations and VA_DEH presented a higher activity than the VA_AEH. The promising cytotoxic potential of VA_DEH lead to the ex vivo skin permeation assay with 2.73 ± 0.19 µg/cm<sup>2</sup> of chlorogenic acid, which permeated at 8 h, showing a transdermal potential. These in vitro results support the idea that VA_DEH is a novel promising candidate for mistletoe therapy. Therefore, further in vivo and pre-clinical experiments should be performed to evaluate the safety and efficacy of this new dermic delivery system.https://www.mdpi.com/1999-4923/14/1/37mistletoe<i>Viscum album</i>thermoresponsive hydrogelrheologystabilitytoxicity test |
| spellingShingle | João V. D. C. Batista Ana Paula S. Matos Adriana P. Oliveria Eduardo Ricci Júnior Zaida M. Freitas Catarina A. Oliveira Helena K. Toma Marcia A. M. Capella Leandro M. Rocha Ulrike Weissenstein Stephan Baumgartner Carla Holandino Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity Pharmaceutics mistletoe <i>Viscum album</i> thermoresponsive hydrogel rheology stability toxicity test |
| title | Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity |
| title_full | Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity |
| title_fullStr | Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity |
| title_full_unstemmed | Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity |
| title_short | Thermoresponsive Hydrogel Containing <i>Viscum album</i> Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity |
| title_sort | thermoresponsive hydrogel containing i viscum album i extract for topic and transdermal use development stability and cytotoxicity activity |
| topic | mistletoe <i>Viscum album</i> thermoresponsive hydrogel rheology stability toxicity test |
| url | https://www.mdpi.com/1999-4923/14/1/37 |
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