Kaempferol alleviates oxidative stress injury of human keratinocytes
Objective To explore the antioxidant effect and mechanism of kaempferol(KP), a component of Vernonia anthelmintica (L.) Willd., in human immortalized keratinocytes (HaCaT). Methods Using selective reaction/multiple response monitoring technique for quantitative analysis of the flavonoids in the...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-08-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210200.pdf |
| _version_ | 1797365768423211008 |
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| author | ZOU Xue-lian, HU Wen, LEI Zi-xian, WANG Hong-juan, XU Chen, Halina HAIRUOLA, KANG Xiao-jing |
| author_facet | ZOU Xue-lian, HU Wen, LEI Zi-xian, WANG Hong-juan, XU Chen, Halina HAIRUOLA, KANG Xiao-jing |
| author_sort | ZOU Xue-lian, HU Wen, LEI Zi-xian, WANG Hong-juan, XU Chen, Halina HAIRUOLA, KANG Xiao-jing |
| collection | DOAJ |
| description | Objective To explore the antioxidant effect and mechanism of kaempferol(KP), a component of Vernonia anthelmintica (L.) Willd., in human immortalized keratinocytes (HaCaT). Methods Using selective reaction/multiple response monitoring technique for quantitative analysis of the flavonoids in the seeds of Vernonia anthelmintica (L.) Willd.. HaCaT cells were divided into control group, model group(HaCaT cells were treated with AAPH to establish the oxidative stress model), low-, middle- and high-dose KP intervention groups.CCK-8 assay for proliferation, RT-qPCR was used for checking the expression of Nrf2, HO-1, NQO1, GCLC and GCLM mRNA, Western blot was used for checking the expression of Nrf2 protein. Results 1)The content of total flavonoids of Vernonia anthelmintica (L.) Willd.. was 0.97 g/100 g, and a total of 36 flavonoid compounds were quantified, the content of KP was 62.96 ng/100 g. 2)The optimum concentration of AAPH for constructing oxidative stress model was 25 mmol/L, and when the concentration of KP was 0-40 μmol/L, no obvious cytotoxicity was found. 3)Low-dose KP increased the expression of Nrf2, HO-1 and GCLC mRNA. Among them, the expression of HO-1 changed most significantly. 4)KP increased the expression of Nrf2 protein in a concentration-dependent manner. Conclusions A component of Vernonia anthelmintica (L.) Willd., reduces the oxidative stress injury of human keratinocytes, the mechanism might be related to the up-regulation of Nrf2 expression. |
| first_indexed | 2024-03-08T16:53:39Z |
| format | Article |
| id | doaj.art-185ff510021f488da75f2ca0958858ca |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:39Z |
| publishDate | 2021-08-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-185ff510021f488da75f2ca0958858ca2024-01-05T03:06:25ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-08-0141811031108Kaempferol alleviates oxidative stress injury of human keratinocytesZOU Xue-lian, HU Wen, LEI Zi-xian, WANG Hong-juan, XU Chen, Halina HAIRUOLA, KANG Xiao-jing01. Xinjiang Clinical College,Anhui Medical University,Urumqi 830001;;2. Department of Dermatology,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001;;3. Xinjiang Key Laboratory of Dermatology Research,Department of Science and Technology of Xinjiang Uygur Autonomous Region, Urumqi 830001, ChinaObjective To explore the antioxidant effect and mechanism of kaempferol(KP), a component of Vernonia anthelmintica (L.) Willd., in human immortalized keratinocytes (HaCaT). Methods Using selective reaction/multiple response monitoring technique for quantitative analysis of the flavonoids in the seeds of Vernonia anthelmintica (L.) Willd.. HaCaT cells were divided into control group, model group(HaCaT cells were treated with AAPH to establish the oxidative stress model), low-, middle- and high-dose KP intervention groups.CCK-8 assay for proliferation, RT-qPCR was used for checking the expression of Nrf2, HO-1, NQO1, GCLC and GCLM mRNA, Western blot was used for checking the expression of Nrf2 protein. Results 1)The content of total flavonoids of Vernonia anthelmintica (L.) Willd.. was 0.97 g/100 g, and a total of 36 flavonoid compounds were quantified, the content of KP was 62.96 ng/100 g. 2)The optimum concentration of AAPH for constructing oxidative stress model was 25 mmol/L, and when the concentration of KP was 0-40 μmol/L, no obvious cytotoxicity was found. 3)Low-dose KP increased the expression of Nrf2, HO-1 and GCLC mRNA. Among them, the expression of HO-1 changed most significantly. 4)KP increased the expression of Nrf2 protein in a concentration-dependent manner. Conclusions A component of Vernonia anthelmintica (L.) Willd., reduces the oxidative stress injury of human keratinocytes, the mechanism might be related to the up-regulation of Nrf2 expression.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210200.pdfvernonia anthelmintica (l.) willd.|kaempferol|vitiligo|oxidative stress|keratinocytes |
| spellingShingle | ZOU Xue-lian, HU Wen, LEI Zi-xian, WANG Hong-juan, XU Chen, Halina HAIRUOLA, KANG Xiao-jing Kaempferol alleviates oxidative stress injury of human keratinocytes Jichu yixue yu linchuang vernonia anthelmintica (l.) willd.|kaempferol|vitiligo|oxidative stress|keratinocytes |
| title | Kaempferol alleviates oxidative stress injury of human keratinocytes |
| title_full | Kaempferol alleviates oxidative stress injury of human keratinocytes |
| title_fullStr | Kaempferol alleviates oxidative stress injury of human keratinocytes |
| title_full_unstemmed | Kaempferol alleviates oxidative stress injury of human keratinocytes |
| title_short | Kaempferol alleviates oxidative stress injury of human keratinocytes |
| title_sort | kaempferol alleviates oxidative stress injury of human keratinocytes |
| topic | vernonia anthelmintica (l.) willd.|kaempferol|vitiligo|oxidative stress|keratinocytes |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210200.pdf |
| work_keys_str_mv | AT zouxuelianhuwenleizixianwanghongjuanxuchenhalinahairuolakangxiaojing kaempferolalleviatesoxidativestressinjuryofhumankeratinocytes |