Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight
Fusarium head blight (FHB), a notorious plant disease caused by Fusarium graminearum (F. graminearum), is severely harmful to wheat production, resulting in a decline in grain quality and yield. In order to develop novel control strategies, metabolomics has been increasingly used to characterize mor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.762605/full |
| _version_ | 1818995834335789056 |
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| author | Peiying Zhao Shubo Gu Chao Han Yaru Lu Chunyang Ma Jichun Tian Jianjie Bi Zhiying Deng Qunqing Wang Qunqing Wang Qian Xu Qian Xu |
| author_facet | Peiying Zhao Shubo Gu Chao Han Yaru Lu Chunyang Ma Jichun Tian Jianjie Bi Zhiying Deng Qunqing Wang Qunqing Wang Qian Xu Qian Xu |
| author_sort | Peiying Zhao |
| collection | DOAJ |
| description | Fusarium head blight (FHB), a notorious plant disease caused by Fusarium graminearum (F. graminearum), is severely harmful to wheat production, resulting in a decline in grain quality and yield. In order to develop novel control strategies, metabolomics has been increasingly used to characterize more comprehensive profiles of the mechanisms of underlying plant-pathogen interactions. In this research, untargeted and targeted metabolomics were used to analyze the metabolite differences between two wheat varieties, the resistant genotype Sumai 3 and the susceptible genotype Shannong 20, after F. graminearum inoculation. The untargeted metabolomics results showed that differential amino acid metabolic pathways existed in Sumai 3 and Shannong 20 after F. graminearum infection. Additionally, some of the amino acid contents changed greatly in different cultivars when infected with F. graminearum. Exogenous application of amino acids and F. graminearum inoculation assay showed that proline (Pro) and alanine (Ala) increased wheat resistance to FHB, while cysteine (Cys) aggravated the susceptibility. This study provides an initial insight into the metabolite differences of two wheat cultivars under the stress of F. graminearum. Moreover, the method of optimization metabolite extraction presents an effective and feasible strategy to explore the understanding of the mechanisms involved in the FHB resistance. |
| first_indexed | 2024-12-20T21:20:08Z |
| format | Article |
| id | doaj.art-186444543984420a8617c1ab804d66d9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T21:20:08Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-186444543984420a8617c1ab804d66d92022-12-21T19:26:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-11-011210.3389/fpls.2021.762605762605Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head BlightPeiying Zhao0Shubo Gu1Chao Han2Yaru Lu3Chunyang Ma4Jichun Tian5Jianjie Bi6Zhiying Deng7Qunqing Wang8Qunqing Wang9Qian Xu10Qian Xu11College of Agronomy, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaShandong Province Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaShandong Province Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaCollege of Agronomy, Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaFusarium head blight (FHB), a notorious plant disease caused by Fusarium graminearum (F. graminearum), is severely harmful to wheat production, resulting in a decline in grain quality and yield. In order to develop novel control strategies, metabolomics has been increasingly used to characterize more comprehensive profiles of the mechanisms of underlying plant-pathogen interactions. In this research, untargeted and targeted metabolomics were used to analyze the metabolite differences between two wheat varieties, the resistant genotype Sumai 3 and the susceptible genotype Shannong 20, after F. graminearum inoculation. The untargeted metabolomics results showed that differential amino acid metabolic pathways existed in Sumai 3 and Shannong 20 after F. graminearum infection. Additionally, some of the amino acid contents changed greatly in different cultivars when infected with F. graminearum. Exogenous application of amino acids and F. graminearum inoculation assay showed that proline (Pro) and alanine (Ala) increased wheat resistance to FHB, while cysteine (Cys) aggravated the susceptibility. This study provides an initial insight into the metabolite differences of two wheat cultivars under the stress of F. graminearum. Moreover, the method of optimization metabolite extraction presents an effective and feasible strategy to explore the understanding of the mechanisms involved in the FHB resistance.https://www.frontiersin.org/articles/10.3389/fpls.2021.762605/fullFusarium head blightmetabolomicswheatamino acidsdisease resistance |
| spellingShingle | Peiying Zhao Shubo Gu Chao Han Yaru Lu Chunyang Ma Jichun Tian Jianjie Bi Zhiying Deng Qunqing Wang Qunqing Wang Qian Xu Qian Xu Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight Frontiers in Plant Science Fusarium head blight metabolomics wheat amino acids disease resistance |
| title | Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight |
| title_full | Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight |
| title_fullStr | Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight |
| title_full_unstemmed | Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight |
| title_short | Targeted and Untargeted Metabolomics Profiling of Wheat Reveals Amino Acids Increase Resistance to Fusarium Head Blight |
| title_sort | targeted and untargeted metabolomics profiling of wheat reveals amino acids increase resistance to fusarium head blight |
| topic | Fusarium head blight metabolomics wheat amino acids disease resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.762605/full |
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