Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes
Interest in using an antimicrobial photodynamic treatment (aPDT) for the microbial decontamination of food has been growing. In this study, quercetin, a substance found ubiquitously in plants, was used as a novel exogenous photosensitizer with 405 nm blue light (BL) for the aPDT on foodborne pathoge...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122002544 |
| _version_ | 1827919357021257728 |
|---|---|
| author | In-Hwan Lee Soo-Hwan Kim Dong-Hyun Kang |
| author_facet | In-Hwan Lee Soo-Hwan Kim Dong-Hyun Kang |
| author_sort | In-Hwan Lee |
| collection | DOAJ |
| description | Interest in using an antimicrobial photodynamic treatment (aPDT) for the microbial decontamination of food has been growing. In this study, quercetin, a substance found ubiquitously in plants, was used as a novel exogenous photosensitizer with 405 nm blue light (BL) for the aPDT on foodborne pathogens, and the inactivation mechanism was elucidated. The inactivation of Escherichia coli O157:H7 and Listeria monocytogenes in PBS solution by the quercetin and BL combination treatment reached a log reduction of 6.2 and more than 7.55 at 80 J/cm2 (68 min 21 s), respectively. When EDTA was added to investigate the reason for different resistance between two bacteria, the effect of aPDT was enhanced against E. coli O157:H7 but not L. monocytogenes. This result indicated that the lipopolysaccharide of Gram-negative bacteria operated as a protective barrier. It was experimentally demonstrated that quercetin generated the superoxide anion and hydrogen peroxide as the reactive oxygen species that oxidize and inactivate cell components. The damage to the bacterial cell membrane by aPDT was evaluated by propidium iodide, where the membrane integrity significantly (P < 0.05) decreased from 40 J/cm2 compared to control. In addition, DNA integrity of bacteria was significantly (P < 0.05) more decreased after aPDT than BL treatment. The inactivation results could be applied in liquid food industries for decontamination of foodborne pathogens, and the mechanisms data was potentially utilized for further studies about aPDT using quercetin. |
| first_indexed | 2024-03-13T03:56:36Z |
| format | Article |
| id | doaj.art-1865e4b2ed72414799053631af364a62 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-13T03:56:36Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-1865e4b2ed72414799053631af364a622023-06-22T05:04:50ZengElsevierCurrent Research in Food Science2665-92712023-01-016100428Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenesIn-Hwan Lee0Soo-Hwan Kim1Dong-Hyun Kang2Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of KoreaDepartment of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of KoreaDepartment of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea; Institutes of Green Bio Science and Technology, Seoul National University, Pyeong-Chang, Gangwon-do, 25354, Republic of Korea; Corresponding author. Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.Interest in using an antimicrobial photodynamic treatment (aPDT) for the microbial decontamination of food has been growing. In this study, quercetin, a substance found ubiquitously in plants, was used as a novel exogenous photosensitizer with 405 nm blue light (BL) for the aPDT on foodborne pathogens, and the inactivation mechanism was elucidated. The inactivation of Escherichia coli O157:H7 and Listeria monocytogenes in PBS solution by the quercetin and BL combination treatment reached a log reduction of 6.2 and more than 7.55 at 80 J/cm2 (68 min 21 s), respectively. When EDTA was added to investigate the reason for different resistance between two bacteria, the effect of aPDT was enhanced against E. coli O157:H7 but not L. monocytogenes. This result indicated that the lipopolysaccharide of Gram-negative bacteria operated as a protective barrier. It was experimentally demonstrated that quercetin generated the superoxide anion and hydrogen peroxide as the reactive oxygen species that oxidize and inactivate cell components. The damage to the bacterial cell membrane by aPDT was evaluated by propidium iodide, where the membrane integrity significantly (P < 0.05) decreased from 40 J/cm2 compared to control. In addition, DNA integrity of bacteria was significantly (P < 0.05) more decreased after aPDT than BL treatment. The inactivation results could be applied in liquid food industries for decontamination of foodborne pathogens, and the mechanisms data was potentially utilized for further studies about aPDT using quercetin.http://www.sciencedirect.com/science/article/pii/S2665927122002544QuercetinPhotodynamic treatmentReactive oxygen species405 nm blue LightFoodborne pathogens |
| spellingShingle | In-Hwan Lee Soo-Hwan Kim Dong-Hyun Kang Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes Current Research in Food Science Quercetin Photodynamic treatment Reactive oxygen species 405 nm blue Light Foodborne pathogens |
| title | Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes |
| title_full | Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes |
| title_fullStr | Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes |
| title_full_unstemmed | Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes |
| title_short | Quercetin mediated antimicrobial photodynamic treatment using blue light on Escherichia coli O157:H7 and Listeria monocytogenes |
| title_sort | quercetin mediated antimicrobial photodynamic treatment using blue light on escherichia coli o157 h7 and listeria monocytogenes |
| topic | Quercetin Photodynamic treatment Reactive oxygen species 405 nm blue Light Foodborne pathogens |
| url | http://www.sciencedirect.com/science/article/pii/S2665927122002544 |
| work_keys_str_mv | AT inhwanlee quercetinmediatedantimicrobialphotodynamictreatmentusingbluelightonescherichiacolio157h7andlisteriamonocytogenes AT soohwankim quercetinmediatedantimicrobialphotodynamictreatmentusingbluelightonescherichiacolio157h7andlisteriamonocytogenes AT donghyunkang quercetinmediatedantimicrobialphotodynamictreatmentusingbluelightonescherichiacolio157h7andlisteriamonocytogenes |