| Summary: | GYF (glycine-tyrosine-phenylalanine)-domain-containing proteins, which were reported to participate in many aspects of biological processes in yeast and animals, are highly conserved adaptor proteins existing in almost all eukaryotes. Our previous study revealed that GYF protein MUSE11/EXA1 is involved in nucleotide-binding leucine-rich repeat (NLR) receptor-mediated defense in <i>Arabidopsis thaliana</i>. However, the GYF-domain encoding homologous genes are still not clear in other plants. Here, we performed genome-wide identification of <i>GYF-domain encoding genes</i> (<i>GYFs</i>) from <i>Brassica napus</i> and its parental species, <i>Brassica rapa</i> and <i>Brassica oleracea</i>. As a result, 26 GYFs of <i>B. napus</i> (<i>BnaGYFs</i>), 11 <i>GYFs</i> of <i>B. rapa</i> (<i>BraGYFs</i>), and <i>14 GYFs</i> of <i>B. oleracea</i> (<i>BolGYFs</i>) together with 10 <i>A. thaliana</i> (<i>AtGYF</i>s) were identified, respectively. We, then, conducted gene structure, motif, cis-acting elements, duplication, chromosome localization, and phylogenetic analysis of these genes. Gene structure analysis indicated the diversity of the exon numbers of these genes. We found that the defense and stress responsiveness element existed in 23 genes and also identified 10 motifs in these GYF proteins. Chromosome localization exhibited a similar distribution of <i>BnaGYFs</i> with <i>BraGYFs</i> or <i>BolGYFs</i> in their respective genomes. The phylogenetic and gene collinearity analysis showed the evolutionary conservation of <i>GYFs</i> among <i>B. napus</i> and its parental species as well as <i>Arabidopsis</i>. These 61 identified GYF domain proteins can be classified into seven groups according to their sequence similarity. Expression of <i>BnaGYFs</i> induced by <i>Sclerotinia sclerotiorum</i> provided five highly upregulated genes and five highly downregulated genes, which might be candidates for further research of plant–fungal interaction in <i>B. napus</i>.
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