Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats
BackgroundBoth animal studies and prospective observational studies on patients with neurodegenerative disease have reported a positive link between oral diseases and cognitive function. However, the effect of early tooth loss on hippocampal morphology remains unknown.MethodsIn this study, 6-week-ol...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.839622/full |
| _version_ | 1811303479025598464 |
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| author | Jiangqi Hu Xiaoyu Wang Wei Kong Qingsong Jiang |
| author_facet | Jiangqi Hu Xiaoyu Wang Wei Kong Qingsong Jiang |
| author_sort | Jiangqi Hu |
| collection | DOAJ |
| description | BackgroundBoth animal studies and prospective observational studies on patients with neurodegenerative disease have reported a positive link between oral diseases and cognitive function. However, the effect of early tooth loss on hippocampal morphology remains unknown.MethodsIn this study, 6-week-old, male, juvenile Sprague–Dawley (SD) rats were randomized into the control (C) and tooth loss (TL) groups. In the TL group, all right maxillary molars of SD rats were extracted, while in the C group, no teeth were extracted. After 3 months, the learning and memory behavior were examined by Morris Water Maze (MWM), and the protein expression and mechanic signaling pathways were analyzed by real-time polymerase chain reaction, and cresyl violet staining.ResultsTwo days after the operation, the body weight of both groups recovered and gradually returned to the level before operation. Three months after tooth extraction, the completion time of the C group in the MWM was significantly shorter than the TL group. The mRNA expression of BDNF, TrkB, AKT1, and NR2B in the C group were significantly higher than in the TL group. The pyramidal neurons in the TL group was fewer than in the C group.ConclusionTooth loss in the juvenile SD rats will reduce the number of pyramidal neurons in the hippocampus, inhibit the expression of BDNF, TrkB, AKT1, and NR2B, and eventually lead to cognitive dysfunction. |
| first_indexed | 2024-04-13T07:47:39Z |
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| id | doaj.art-1868ac1dd02a4ce2a121c599792c3704 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-13T07:47:39Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj.art-1868ac1dd02a4ce2a121c599792c37042022-12-22T02:55:37ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-04-011610.3389/fnins.2022.839622839622Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley RatsJiangqi Hu0Xiaoyu Wang1Wei Kong2Qingsong Jiang3Beijing Stomatological Hospital, Capital Medical University, Beijing, ChinaBeijing Stomatological Hospital, Capital Medical University, Beijing, ChinaKey Laboratory of Molecular Medicine and Biotherapy, Department of Biology, School of Life Sciences, Beijing Institute of Technology, Beijing, ChinaBeijing Stomatological Hospital, Capital Medical University, Beijing, ChinaBackgroundBoth animal studies and prospective observational studies on patients with neurodegenerative disease have reported a positive link between oral diseases and cognitive function. However, the effect of early tooth loss on hippocampal morphology remains unknown.MethodsIn this study, 6-week-old, male, juvenile Sprague–Dawley (SD) rats were randomized into the control (C) and tooth loss (TL) groups. In the TL group, all right maxillary molars of SD rats were extracted, while in the C group, no teeth were extracted. After 3 months, the learning and memory behavior were examined by Morris Water Maze (MWM), and the protein expression and mechanic signaling pathways were analyzed by real-time polymerase chain reaction, and cresyl violet staining.ResultsTwo days after the operation, the body weight of both groups recovered and gradually returned to the level before operation. Three months after tooth extraction, the completion time of the C group in the MWM was significantly shorter than the TL group. The mRNA expression of BDNF, TrkB, AKT1, and NR2B in the C group were significantly higher than in the TL group. The pyramidal neurons in the TL group was fewer than in the C group.ConclusionTooth loss in the juvenile SD rats will reduce the number of pyramidal neurons in the hippocampus, inhibit the expression of BDNF, TrkB, AKT1, and NR2B, and eventually lead to cognitive dysfunction.https://www.frontiersin.org/articles/10.3389/fnins.2022.839622/fulltooth losscognitive dysfunctionAlzheimer’s disease (AD)hippocampusbrain-derived neurotrophic factor (BDNF) |
| spellingShingle | Jiangqi Hu Xiaoyu Wang Wei Kong Qingsong Jiang Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats Frontiers in Neuroscience tooth loss cognitive dysfunction Alzheimer’s disease (AD) hippocampus brain-derived neurotrophic factor (BDNF) |
| title | Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats |
| title_full | Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats |
| title_fullStr | Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats |
| title_full_unstemmed | Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats |
| title_short | Tooth Loss Suppresses Hippocampal Neurogenesis and Leads to Cognitive Dysfunction in Juvenile Sprague–Dawley Rats |
| title_sort | tooth loss suppresses hippocampal neurogenesis and leads to cognitive dysfunction in juvenile sprague dawley rats |
| topic | tooth loss cognitive dysfunction Alzheimer’s disease (AD) hippocampus brain-derived neurotrophic factor (BDNF) |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.839622/full |
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