The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio)
Bacillus spp. as probiotics have been widely used in the aquaculture industry. This study was designed to investigate the dietary supplementation of Bacillus cereus QSI-1 with quorum quenching activity, on intestinal barrier and expression levels of innate immune genes. After 14 d of feeding, fish f...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513420301307 |
| _version_ | 1811322338569879552 |
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| author | Yuanhe Jiang Shuxin Zhou Emmanuel Konadu Sarkodie Weihua Chu |
| author_facet | Yuanhe Jiang Shuxin Zhou Emmanuel Konadu Sarkodie Weihua Chu |
| author_sort | Yuanhe Jiang |
| collection | DOAJ |
| description | Bacillus spp. as probiotics have been widely used in the aquaculture industry. This study was designed to investigate the dietary supplementation of Bacillus cereus QSI-1 with quorum quenching activity, on intestinal barrier and expression levels of innate immune genes. After 14 d of feeding, fish fed with Bacillus cereus QSI-1 exhibited a significant down-regulated gene expression of TNF, IL-8, and IL-10 in the intestinal mucosa, but up-regulated tight junction (TJ) proteins zonula occludens-1 (ZO-1) and occludin and adaptor protein myeloid differentiation factor 88 (MyD88) transcription. This is an indication that Bacillus cereus QSI-1 could enhance intestinal barrier function and activate the non-specific immune system of Crucian carp. At the end of the feeding trial, the Crucian carps in each group were anally infected with A. hydrophila YJ-1. The results showed that Bacillus cereus QSI-1 could prevent the inflammatory response caused by A. hydrophila infection. Histological (integrity of intestinal villi) and qPCR analyses suggested that Bacillus cereus QSI-1 could improve the intestinal barrier function. Taken together, these findings indicate that Bacillus cereus QSI-1 could increase the intestinal epithelial barrier function and innate immune function, and also has the potential to be an alternative agent instead of antibiotics in feed supplements in aquaculture. |
| first_indexed | 2024-04-13T13:33:26Z |
| format | Article |
| id | doaj.art-186b452f11c84e51af46e1b4f7c04c60 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-13T13:33:26Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-186b452f11c84e51af46e1b4f7c04c602022-12-22T02:44:51ZengElsevierAquaculture Reports2352-51342020-07-0117100356The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio)Yuanhe Jiang0Shuxin Zhou1Emmanuel Konadu Sarkodie2Weihua Chu3Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, ChinaDepartment of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, ChinaDepartment of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, ChinaCorresponding author.; Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, ChinaBacillus spp. as probiotics have been widely used in the aquaculture industry. This study was designed to investigate the dietary supplementation of Bacillus cereus QSI-1 with quorum quenching activity, on intestinal barrier and expression levels of innate immune genes. After 14 d of feeding, fish fed with Bacillus cereus QSI-1 exhibited a significant down-regulated gene expression of TNF, IL-8, and IL-10 in the intestinal mucosa, but up-regulated tight junction (TJ) proteins zonula occludens-1 (ZO-1) and occludin and adaptor protein myeloid differentiation factor 88 (MyD88) transcription. This is an indication that Bacillus cereus QSI-1 could enhance intestinal barrier function and activate the non-specific immune system of Crucian carp. At the end of the feeding trial, the Crucian carps in each group were anally infected with A. hydrophila YJ-1. The results showed that Bacillus cereus QSI-1 could prevent the inflammatory response caused by A. hydrophila infection. Histological (integrity of intestinal villi) and qPCR analyses suggested that Bacillus cereus QSI-1 could improve the intestinal barrier function. Taken together, these findings indicate that Bacillus cereus QSI-1 could increase the intestinal epithelial barrier function and innate immune function, and also has the potential to be an alternative agent instead of antibiotics in feed supplements in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513420301307Bacillus cereus QSI-1Crucian carpIntestinal barrierGut innate immune |
| spellingShingle | Yuanhe Jiang Shuxin Zhou Emmanuel Konadu Sarkodie Weihua Chu The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) Aquaculture Reports Bacillus cereus QSI-1 Crucian carp Intestinal barrier Gut innate immune |
| title | The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) |
| title_full | The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) |
| title_fullStr | The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) |
| title_full_unstemmed | The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) |
| title_short | The effects of Bacillus cereus QSI-1 on intestinal barrier function and mucosal gene transcription in Crucian carp (Carassius auratus gibelio) |
| title_sort | effects of bacillus cereus qsi 1 on intestinal barrier function and mucosal gene transcription in crucian carp carassius auratus gibelio |
| topic | Bacillus cereus QSI-1 Crucian carp Intestinal barrier Gut innate immune |
| url | http://www.sciencedirect.com/science/article/pii/S2352513420301307 |
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