Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria
Abstract Background Populus euphratica Olivier is a kind of tree capable of growing in extremely arid desert and semi-desert environments. In this study, a culture-dependent method was used to analyze the bacterial diversity of stem liquid of P. euphratica and resina of P. euphratica, and to further...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-022-02731-7 |
| _version_ | 1797973681964580864 |
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| author | Nusratgul Anwar Yuhang Jiang Wenbo Ma Yuhao Yao Jue Li Gulibahaer Ababaikeli Guoqiang Li Ting Ma |
| author_facet | Nusratgul Anwar Yuhang Jiang Wenbo Ma Yuhao Yao Jue Li Gulibahaer Ababaikeli Guoqiang Li Ting Ma |
| author_sort | Nusratgul Anwar |
| collection | DOAJ |
| description | Abstract Background Populus euphratica Olivier is a kind of tree capable of growing in extremely arid desert and semi-desert environments. In this study, a culture-dependent method was used to analyze the bacterial diversity of stem liquid of P. euphratica and resina of P. euphratica, and to further evaluate plant growth promoting (PGP) activity. Results A total of 434 bacteria were isolated from stem fluid and resina of P. euphratica in Ebinur Lake Wetland Nature Reserve and Mulei Primitive forest. The results of taxonomic composition analysis shows that Gammaproteobacteria, Firmicutes, and Actinobacteria_c are the three dominant groups in all the communities, and the representative genera are Bacillus, Nesterenkonia and Halomonas. The diversity analysis shows that the culturable bacterial community diversity of P. euphratica in Ebinur Lake Wetland Nature Reserve is higher than that in Mulei Primitive forest, and the bacterial community diversity of P. euphratica stem fluid is higher than that of resina. According to PGP activity evaluation, 158 functional bacteria with plant growth promoting potential were screened. Among them, 61 strains havephosphorus solubilizing abilities, 80 strains have potassium solubilizing abilities, 32 strains have nitrogen fixation abilities, and 151 strains have iron ammonia salt utilization abilities. The germination rate, plant height, and dry weight of the maize seedlings treated with strains BB33-1, TC10 and RC6 are significantly higher than those of the control group. Conclusion In this study, a large number of culturable bacteria were isolated from P. euphratica, which provides new functional bacteria sources for promoting plant growth. |
| first_indexed | 2024-04-11T04:08:12Z |
| format | Article |
| id | doaj.art-186d7a9b1b984627943b225326eedbf4 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T04:08:12Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-186d7a9b1b984627943b225326eedbf42023-01-01T12:14:14ZengBMCBMC Microbiology1471-21802022-12-0122111610.1186/s12866-022-02731-7Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteriaNusratgul Anwar0Yuhang Jiang1Wenbo Ma2Yuhao Yao3Jue Li4Gulibahaer Ababaikeli5Guoqiang Li6Ting Ma7Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityCollege of Life Sciences, Xinjiang Normal UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityAbstract Background Populus euphratica Olivier is a kind of tree capable of growing in extremely arid desert and semi-desert environments. In this study, a culture-dependent method was used to analyze the bacterial diversity of stem liquid of P. euphratica and resina of P. euphratica, and to further evaluate plant growth promoting (PGP) activity. Results A total of 434 bacteria were isolated from stem fluid and resina of P. euphratica in Ebinur Lake Wetland Nature Reserve and Mulei Primitive forest. The results of taxonomic composition analysis shows that Gammaproteobacteria, Firmicutes, and Actinobacteria_c are the three dominant groups in all the communities, and the representative genera are Bacillus, Nesterenkonia and Halomonas. The diversity analysis shows that the culturable bacterial community diversity of P. euphratica in Ebinur Lake Wetland Nature Reserve is higher than that in Mulei Primitive forest, and the bacterial community diversity of P. euphratica stem fluid is higher than that of resina. According to PGP activity evaluation, 158 functional bacteria with plant growth promoting potential were screened. Among them, 61 strains havephosphorus solubilizing abilities, 80 strains have potassium solubilizing abilities, 32 strains have nitrogen fixation abilities, and 151 strains have iron ammonia salt utilization abilities. The germination rate, plant height, and dry weight of the maize seedlings treated with strains BB33-1, TC10 and RC6 are significantly higher than those of the control group. Conclusion In this study, a large number of culturable bacteria were isolated from P. euphratica, which provides new functional bacteria sources for promoting plant growth.https://doi.org/10.1186/s12866-022-02731-7Culturable bacteriaPlant-promoting BacteriaPopulus euphratica |
| spellingShingle | Nusratgul Anwar Yuhang Jiang Wenbo Ma Yuhao Yao Jue Li Gulibahaer Ababaikeli Guoqiang Li Ting Ma Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria BMC Microbiology Culturable bacteria Plant-promoting Bacteria Populus euphratica |
| title | Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria |
| title_full | Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria |
| title_fullStr | Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria |
| title_full_unstemmed | Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria |
| title_short | Culturable bacteria diversity in stem liquid and resina from Populus euphratica and screening of plant growth-promoting bacteria |
| title_sort | culturable bacteria diversity in stem liquid and resina from populus euphratica and screening of plant growth promoting bacteria |
| topic | Culturable bacteria Plant-promoting Bacteria Populus euphratica |
| url | https://doi.org/10.1186/s12866-022-02731-7 |
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