A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice
Early life stress (ELS) in developing children has been linked to physical and psychological sequelae in adulthood. In the present study, we investigated the effects of ELS on brain and behavioral development by establishing a novel ELS model that combined the maternal separation paradigm and mesh p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4688 |
| _version_ | 1797615137047183360 |
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| author | Hyun Seung Shin Soo Min Choi Seung Hyun Lee Ha Jung Moon Eui-Man Jung |
| author_facet | Hyun Seung Shin Soo Min Choi Seung Hyun Lee Ha Jung Moon Eui-Man Jung |
| author_sort | Hyun Seung Shin |
| collection | DOAJ |
| description | Early life stress (ELS) in developing children has been linked to physical and psychological sequelae in adulthood. In the present study, we investigated the effects of ELS on brain and behavioral development by establishing a novel ELS model that combined the maternal separation paradigm and mesh platform condition. We found that the novel ELS model caused anxiety- and depression-like behaviors and induced social deficits and memory impairment in the offspring of mice. In particular, the novel ELS model induced more enhanced depression-like behavior and memory impairment than the maternal separation model, which is the established ELS model. Furthermore, the novel ELS caused upregulation of arginine vasopressin expression and downregulation of GABAergic interneuron markers, such as parvalbumin (<i>PV</i>), vasoactive intestinal peptide, and calbindin-D<sub>28k</sub> (<i>CaBP-28k</i>), in the brains of the mice. Finally, the offspring in the novel ELS model showed a decreased number of cortical PV-, CaBP-28k-positive cells and an increased number of cortical ionized calcium-binding adaptors-positive cells in their brains compared to mice in the established ELS model. Collectively, these results indicated that the novel ELS model induced more negative effects on brain and behavioral development than the established ELS model. |
| first_indexed | 2024-03-11T07:22:16Z |
| format | Article |
| id | doaj.art-186f49b5911741f587bff297333f122a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:22:16Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-186f49b5911741f587bff297333f122a2023-11-17T07:52:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245468810.3390/ijms24054688A Novel Early Life Stress Model Affects Brain Development and Behavior in MiceHyun Seung Shin0Soo Min Choi1Seung Hyun Lee2Ha Jung Moon3Eui-Man Jung4Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of KoreaDepartment of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of KoreaDepartment of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of KoreaDepartment of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of KoreaDepartment of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of KoreaEarly life stress (ELS) in developing children has been linked to physical and psychological sequelae in adulthood. In the present study, we investigated the effects of ELS on brain and behavioral development by establishing a novel ELS model that combined the maternal separation paradigm and mesh platform condition. We found that the novel ELS model caused anxiety- and depression-like behaviors and induced social deficits and memory impairment in the offspring of mice. In particular, the novel ELS model induced more enhanced depression-like behavior and memory impairment than the maternal separation model, which is the established ELS model. Furthermore, the novel ELS caused upregulation of arginine vasopressin expression and downregulation of GABAergic interneuron markers, such as parvalbumin (<i>PV</i>), vasoactive intestinal peptide, and calbindin-D<sub>28k</sub> (<i>CaBP-28k</i>), in the brains of the mice. Finally, the offspring in the novel ELS model showed a decreased number of cortical PV-, CaBP-28k-positive cells and an increased number of cortical ionized calcium-binding adaptors-positive cells in their brains compared to mice in the established ELS model. Collectively, these results indicated that the novel ELS model induced more negative effects on brain and behavioral development than the established ELS model.https://www.mdpi.com/1422-0067/24/5/4688early life stressbehaviormicecalbindin-D<sub>28k</sub>parvalbumin |
| spellingShingle | Hyun Seung Shin Soo Min Choi Seung Hyun Lee Ha Jung Moon Eui-Man Jung A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice International Journal of Molecular Sciences early life stress behavior mice calbindin-D<sub>28k</sub> parvalbumin |
| title | A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice |
| title_full | A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice |
| title_fullStr | A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice |
| title_full_unstemmed | A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice |
| title_short | A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice |
| title_sort | novel early life stress model affects brain development and behavior in mice |
| topic | early life stress behavior mice calbindin-D<sub>28k</sub> parvalbumin |
| url | https://www.mdpi.com/1422-0067/24/5/4688 |
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