Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate
Mineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) an...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/10/2336 |
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| author | Tamaki Hattori-Sanuki Takeo Karakida Risako Chiba-Ohkuma Yasuo Miake Ryuji Yamamoto Yasuo Yamakoshi Noriyasu Hosoya |
| author_facet | Tamaki Hattori-Sanuki Takeo Karakida Risako Chiba-Ohkuma Yasuo Miake Ryuji Yamamoto Yasuo Yamakoshi Noriyasu Hosoya |
| author_sort | Tamaki Hattori-Sanuki |
| collection | DOAJ |
| description | Mineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) and cloned. DsRed-PPU7 cells were cultured on the MTA disk and cell proliferation, chemotaxis, the effects of growth factors and the gene expression of cells were investigated at the biological, histomorphological and genetic cell levels. Mineralized precipitates formed in the DsRed-PPU7 cells were characterized with crystal structural analysis. DsRed-PPU7 cells proliferated in the central part of the MTA disk until Day 6 and displayed a tendency to move to the outer circumference. Both transforming growth factor beta and bone morphogenetic protein promoted the proliferation and movement of DsRed-PPU7 cells and also enhanced the expression levels of odontoblastic gene differentiation markers. Mineralized precipitates formed in DsRed-PPU7 were composed of calcium and phosphate but its crystals were different in each position. Our investigation showed that DsRed-PPU7 cells in direct contact with the MTA disk could differentiate into odontoblasts by controlling cell–cell and cell–substrate interactions depending on cell adhesion and the surrounding environment of the MTA. |
| first_indexed | 2024-03-10T15:26:17Z |
| format | Article |
| id | doaj.art-1870448dd86441c28ac89df685bfc1ca |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T15:26:17Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1870448dd86441c28ac89df685bfc1ca2023-11-20T17:59:28ZengMDPI AGCells2073-44092020-10-01910233610.3390/cells9102336Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide AggregateTamaki Hattori-Sanuki0Takeo Karakida1Risako Chiba-Ohkuma2Yasuo Miake3Ryuji Yamamoto4Yasuo Yamakoshi5Noriyasu Hosoya6Department of Endodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Anatomy, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanDepartment of Endodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, JapanMineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) and cloned. DsRed-PPU7 cells were cultured on the MTA disk and cell proliferation, chemotaxis, the effects of growth factors and the gene expression of cells were investigated at the biological, histomorphological and genetic cell levels. Mineralized precipitates formed in the DsRed-PPU7 cells were characterized with crystal structural analysis. DsRed-PPU7 cells proliferated in the central part of the MTA disk until Day 6 and displayed a tendency to move to the outer circumference. Both transforming growth factor beta and bone morphogenetic protein promoted the proliferation and movement of DsRed-PPU7 cells and also enhanced the expression levels of odontoblastic gene differentiation markers. Mineralized precipitates formed in DsRed-PPU7 were composed of calcium and phosphate but its crystals were different in each position. Our investigation showed that DsRed-PPU7 cells in direct contact with the MTA disk could differentiate into odontoblasts by controlling cell–cell and cell–substrate interactions depending on cell adhesion and the surrounding environment of the MTA.https://www.mdpi.com/2073-4409/9/10/2336mineral trioxide aggregatedental pulp cellsfluorescent labelingcell chemotaxiscalcium phosphate crystal |
| spellingShingle | Tamaki Hattori-Sanuki Takeo Karakida Risako Chiba-Ohkuma Yasuo Miake Ryuji Yamamoto Yasuo Yamakoshi Noriyasu Hosoya Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate Cells mineral trioxide aggregate dental pulp cells fluorescent labeling cell chemotaxis calcium phosphate crystal |
| title | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
| title_full | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
| title_fullStr | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
| title_full_unstemmed | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
| title_short | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
| title_sort | characterization of living dental pulp cells in direct contact with mineral trioxide aggregate |
| topic | mineral trioxide aggregate dental pulp cells fluorescent labeling cell chemotaxis calcium phosphate crystal |
| url | https://www.mdpi.com/2073-4409/9/10/2336 |
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