Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion
A lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion’s lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2566 |
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| author | Atitaya Hitakarun Maia Kavanagh Williamson Nathamon Yimpring Wannapa Sornjai Nitwara Wikan Christopher J. Arthur Julien Pompon Andrew D. Davidson Duncan R. Smith |
| author_facet | Atitaya Hitakarun Maia Kavanagh Williamson Nathamon Yimpring Wannapa Sornjai Nitwara Wikan Christopher J. Arthur Julien Pompon Andrew D. Davidson Duncan R. Smith |
| author_sort | Atitaya Hitakarun |
| collection | DOAJ |
| description | A lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion’s lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of DENV virions derived from two cell lines (C6/36 and LLC-MK<sub>2</sub>). The results showed distinctive profiles between the two cell types. In the mammalian LLC-MK<sub>2</sub> cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect C6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. For virions from LLC-MK<sub>2</sub> cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). For virions from C6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). These results provide the first lipid description of DENV virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells. |
| first_indexed | 2024-03-09T17:55:53Z |
| format | Article |
| id | doaj.art-1870f9bdc92446f4914287c249bc91ad |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T17:55:53Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1870f9bdc92446f4914287c249bc91ad2023-11-24T10:19:18ZengMDPI AGViruses1999-49152022-11-011411256610.3390/v14112566Cell Type Variability in the Incorporation of Lipids in the Dengue Virus VirionAtitaya Hitakarun0Maia Kavanagh Williamson1Nathamon Yimpring2Wannapa Sornjai3Nitwara Wikan4Christopher J. Arthur5Julien Pompon6Andrew D. Davidson7Duncan R. Smith8Institute of Molecular Biosciences, Mahidol University, Salaya, Bangkok 73170, ThailandSchool of Cellular and Molecular Medicine, University of Bristol, Bristol BS81TD, UKInstitute of Molecular Biosciences, Mahidol University, Salaya, Bangkok 73170, ThailandInstitute of Molecular Biosciences, Mahidol University, Salaya, Bangkok 73170, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Chemistry, Faculty of Science, University of Bristol, Bristol BS81TD, UKMaladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle (MIVEGEC), Institut de Recherche Pour le Développement (IRD), University Montpellier, CNRS, 911, Avenue Agropolis, 34394 Montpellier, FranceSchool of Cellular and Molecular Medicine, University of Bristol, Bristol BS81TD, UKInstitute of Molecular Biosciences, Mahidol University, Salaya, Bangkok 73170, ThailandA lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion’s lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of DENV virions derived from two cell lines (C6/36 and LLC-MK<sub>2</sub>). The results showed distinctive profiles between the two cell types. In the mammalian LLC-MK<sub>2</sub> cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect C6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. For virions from LLC-MK<sub>2</sub> cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). For virions from C6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). These results provide the first lipid description of DENV virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells.https://www.mdpi.com/1999-4915/14/11/2566dengue virusglycerolipidssphingolipidsglycerophospholipidsLLC-MK2 cellsC6/36 cells |
| spellingShingle | Atitaya Hitakarun Maia Kavanagh Williamson Nathamon Yimpring Wannapa Sornjai Nitwara Wikan Christopher J. Arthur Julien Pompon Andrew D. Davidson Duncan R. Smith Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion Viruses dengue virus glycerolipids sphingolipids glycerophospholipids LLC-MK2 cells C6/36 cells |
| title | Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion |
| title_full | Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion |
| title_fullStr | Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion |
| title_full_unstemmed | Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion |
| title_short | Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion |
| title_sort | cell type variability in the incorporation of lipids in the dengue virus virion |
| topic | dengue virus glycerolipids sphingolipids glycerophospholipids LLC-MK2 cells C6/36 cells |
| url | https://www.mdpi.com/1999-4915/14/11/2566 |
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