Multivesicular Liposomes for Glucose-Responsive Insulin Delivery
An intelligent insulin delivery system is highly desirable for diabetes management. Herein, we developed a novel glucose-responsive multivesicular liposome (MVL) for self-regulated insulin delivery using the double emulsion method. Glucose-responsive MVLs could effectively regulate insulin release i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/1/21 |
| _version_ | 1827663510264348672 |
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| author | Guangqu Liu Suping He Yu Ding Cai Chen Qingchun Cai Wei Zhou |
| author_facet | Guangqu Liu Suping He Yu Ding Cai Chen Qingchun Cai Wei Zhou |
| author_sort | Guangqu Liu |
| collection | DOAJ |
| description | An intelligent insulin delivery system is highly desirable for diabetes management. Herein, we developed a novel glucose-responsive multivesicular liposome (MVL) for self-regulated insulin delivery using the double emulsion method. Glucose-responsive MVLs could effectively regulate insulin release in response to fluctuating glucose concentrations in vitro. Notably, in situ released glucose oxidase catalyzed glucose enrichment on the MVL surface, based on the combination of (3-fluoro-4-((octyloxy)carbonyl)phenyl)boronic acid and glucose. The outer MVL membrane was destroyed when triggered by the local acidic and H<sub>2</sub>O<sub>2</sub>-enriched microenvironment induced by glucose oxidase catalysis in situ, followed by the further release of entrapped insulin. Moreover, the Alizarin red probe and molecular docking were used to clarify the glucose-responsive mechanism of MVLs. Utilizing chemically induced type 1 diabetic rats, we demonstrated that the glucose-responsive MVLs could effectively regulate blood glucose levels within a normal range. Our findings suggest that glucose-responsive MVLs with good biocompatibility may have promising applications in diabetes treatment. |
| first_indexed | 2024-03-10T00:43:50Z |
| format | Article |
| id | doaj.art-187163d0c90742cd8ebb55833ec00b5b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T00:43:50Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-187163d0c90742cd8ebb55833ec00b5b2023-11-23T15:02:35ZengMDPI AGPharmaceutics1999-49232021-12-011412110.3390/pharmaceutics14010021Multivesicular Liposomes for Glucose-Responsive Insulin DeliveryGuangqu Liu0Suping He1Yu Ding2Cai Chen3Qingchun Cai4Wei Zhou5Department of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, ChinaAn intelligent insulin delivery system is highly desirable for diabetes management. Herein, we developed a novel glucose-responsive multivesicular liposome (MVL) for self-regulated insulin delivery using the double emulsion method. Glucose-responsive MVLs could effectively regulate insulin release in response to fluctuating glucose concentrations in vitro. Notably, in situ released glucose oxidase catalyzed glucose enrichment on the MVL surface, based on the combination of (3-fluoro-4-((octyloxy)carbonyl)phenyl)boronic acid and glucose. The outer MVL membrane was destroyed when triggered by the local acidic and H<sub>2</sub>O<sub>2</sub>-enriched microenvironment induced by glucose oxidase catalysis in situ, followed by the further release of entrapped insulin. Moreover, the Alizarin red probe and molecular docking were used to clarify the glucose-responsive mechanism of MVLs. Utilizing chemically induced type 1 diabetic rats, we demonstrated that the glucose-responsive MVLs could effectively regulate blood glucose levels within a normal range. Our findings suggest that glucose-responsive MVLs with good biocompatibility may have promising applications in diabetes treatment.https://www.mdpi.com/1999-4923/14/1/21MVLglucose-responsiveinsulinin situ catalysisAlizarin red probemolecular docking |
| spellingShingle | Guangqu Liu Suping He Yu Ding Cai Chen Qingchun Cai Wei Zhou Multivesicular Liposomes for Glucose-Responsive Insulin Delivery Pharmaceutics MVL glucose-responsive insulin in situ catalysis Alizarin red probe molecular docking |
| title | Multivesicular Liposomes for Glucose-Responsive Insulin Delivery |
| title_full | Multivesicular Liposomes for Glucose-Responsive Insulin Delivery |
| title_fullStr | Multivesicular Liposomes for Glucose-Responsive Insulin Delivery |
| title_full_unstemmed | Multivesicular Liposomes for Glucose-Responsive Insulin Delivery |
| title_short | Multivesicular Liposomes for Glucose-Responsive Insulin Delivery |
| title_sort | multivesicular liposomes for glucose responsive insulin delivery |
| topic | MVL glucose-responsive insulin in situ catalysis Alizarin red probe molecular docking |
| url | https://www.mdpi.com/1999-4923/14/1/21 |
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