Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i>
The rise in the number of antibiotic-resistant bacteria has become a serious threat to health, making it important to identify, characterize and optimize new molecules to help us to overcome the infections they cause. It is well known that <i>Acinetobacter baumannii</i> has a significant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/354 |
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| author | Nuria Crua Asensio Javier Macho Rendón Marc Torrent Burgas |
| author_facet | Nuria Crua Asensio Javier Macho Rendón Marc Torrent Burgas |
| author_sort | Nuria Crua Asensio |
| collection | DOAJ |
| description | The rise in the number of antibiotic-resistant bacteria has become a serious threat to health, making it important to identify, characterize and optimize new molecules to help us to overcome the infections they cause. It is well known that <i>Acinetobacter baumannii</i> has a significant capacity to evade the actions of antibacterial drugs, leading to its emergence as one of the bacteria responsible for hospital and community-acquired infections. Nonetheless, how this pathogen infects and survives inside the host cell is unclear. In this study, we analyze the time-resolved transcriptional profile changes observed in human epithelial HeLa cells after infection by <i>A. baumannii</i>, demonstrating how it survives in host cells and starts to replicate 4 h post infection. These findings were achieved by sequencing RNA to obtain a set of Differentially Expressed Genes (DEGs) to understand how bacteria alter the host cells’ environment for their own benefit. We also determine common features observed in this set of genes and identify the protein–protein networks that reveal highly-interacted proteins. The combination of these findings paves the way for the discovery of new antimicrobial candidates for the treatment of multidrug-resistant bacteria. |
| first_indexed | 2024-03-09T04:42:40Z |
| format | Article |
| id | doaj.art-1871b8e0b893405394b5446b6e558732 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:42:40Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1871b8e0b893405394b5446b6e5587322023-12-03T13:19:05ZengMDPI AGMicroorganisms2076-26072021-02-019235410.3390/microorganisms9020354Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i>Nuria Crua Asensio0Javier Macho Rendón1Marc Torrent Burgas2Systems Biology of Infection Laboratory, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, SpainSystems Biology of Infection Laboratory, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, SpainSystems Biology of Infection Laboratory, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, SpainThe rise in the number of antibiotic-resistant bacteria has become a serious threat to health, making it important to identify, characterize and optimize new molecules to help us to overcome the infections they cause. It is well known that <i>Acinetobacter baumannii</i> has a significant capacity to evade the actions of antibacterial drugs, leading to its emergence as one of the bacteria responsible for hospital and community-acquired infections. Nonetheless, how this pathogen infects and survives inside the host cell is unclear. In this study, we analyze the time-resolved transcriptional profile changes observed in human epithelial HeLa cells after infection by <i>A. baumannii</i>, demonstrating how it survives in host cells and starts to replicate 4 h post infection. These findings were achieved by sequencing RNA to obtain a set of Differentially Expressed Genes (DEGs) to understand how bacteria alter the host cells’ environment for their own benefit. We also determine common features observed in this set of genes and identify the protein–protein networks that reveal highly-interacted proteins. The combination of these findings paves the way for the discovery of new antimicrobial candidates for the treatment of multidrug-resistant bacteria.https://www.mdpi.com/2076-2607/9/2/354<i>Acinetobacter baumannii</i>infectionintracellulartranscriptional profile |
| spellingShingle | Nuria Crua Asensio Javier Macho Rendón Marc Torrent Burgas Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> Microorganisms <i>Acinetobacter baumannii</i> infection intracellular transcriptional profile |
| title | Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> |
| title_full | Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> |
| title_fullStr | Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> |
| title_short | Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular <i>Acinetobacter baumannii</i> |
| title_sort | time resolved transcriptional profiling of epithelial cells infected by intracellular i acinetobacter baumannii i |
| topic | <i>Acinetobacter baumannii</i> infection intracellular transcriptional profile |
| url | https://www.mdpi.com/2076-2607/9/2/354 |
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