Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin
Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/7/3218 |
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| author | Rosa Mancinelli Ester Sara Di Filippo Margherita Tumedei Mariangela Marrone Antonella Fontana Valeria Ettorre Silvia Giordani Michele Baldrighi Giovanna Iezzi Adriano Piattelli Stefania Fulle |
| author_facet | Rosa Mancinelli Ester Sara Di Filippo Margherita Tumedei Mariangela Marrone Antonella Fontana Valeria Ettorre Silvia Giordani Michele Baldrighi Giovanna Iezzi Adriano Piattelli Stefania Fulle |
| author_sort | Rosa Mancinelli |
| collection | DOAJ |
| description | Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2; and 10 μg/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process. |
| first_indexed | 2024-03-10T12:37:22Z |
| format | Article |
| id | doaj.art-18726a931bc045c192d1731ae45bddf4 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T12:37:22Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-18726a931bc045c192d1731ae45bddf42023-11-21T14:09:05ZengMDPI AGApplied Sciences2076-34172021-04-01117321810.3390/app11073218Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and MelatoninRosa Mancinelli0Ester Sara Di Filippo1Margherita Tumedei2Mariangela Marrone3Antonella Fontana4Valeria Ettorre5Silvia Giordani6Michele Baldrighi7Giovanna Iezzi8Adriano Piattelli9Stefania Fulle10Department of Neuroscience Imaging and Clinical Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience Imaging and Clinical Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience Imaging and Clinical Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalySchool of Chemical Sciences, Dublin City University, Glasnevin, D09 E432 Dublin 9, IrelandNano Carbon Materials, Italian Institute of Technology, via Morego 30, 16163 Genova, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience Imaging and Clinical Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyEquine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2; and 10 μg/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process.https://www.mdpi.com/2076-3417/11/7/3218hDPSCgraphene-oxideequine bone blockmelatoninosteogenesisbone regeneration |
| spellingShingle | Rosa Mancinelli Ester Sara Di Filippo Margherita Tumedei Mariangela Marrone Antonella Fontana Valeria Ettorre Silvia Giordani Michele Baldrighi Giovanna Iezzi Adriano Piattelli Stefania Fulle Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin Applied Sciences hDPSC graphene-oxide equine bone block melatonin osteogenesis bone regeneration |
| title | Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin |
| title_full | Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin |
| title_fullStr | Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin |
| title_full_unstemmed | Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin |
| title_short | Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin |
| title_sort | human dental pulp stem cell osteogenic differentiation seeded on equine bone block with graphene and melatonin |
| topic | hDPSC graphene-oxide equine bone block melatonin osteogenesis bone regeneration |
| url | https://www.mdpi.com/2076-3417/11/7/3218 |
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