Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation
Asthma is a chronic disease affecting people of all ages. Asthma medications are associated with adverse effects restricting their long-term usage, demanding newer alternative therapies. This study aimed to investigate the anti-asthmatic properties of Ruta graveolens extract and its prepared nano-cu...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016424000185 |
| _version_ | 1797291749686640640 |
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| author | Mohammad H. Alyami Dalia I. Hamdan Heba M.A. Khalil Mohamed A.A. Orabi Nora M. Aborehab Nada Osama Mai M. Abdelhafez Abdulaziz Mohsen Al-Mahallawi Hamad S. Alyami |
| author_facet | Mohammad H. Alyami Dalia I. Hamdan Heba M.A. Khalil Mohamed A.A. Orabi Nora M. Aborehab Nada Osama Mai M. Abdelhafez Abdulaziz Mohsen Al-Mahallawi Hamad S. Alyami |
| author_sort | Mohammad H. Alyami |
| collection | DOAJ |
| description | Asthma is a chronic disease affecting people of all ages. Asthma medications are associated with adverse effects restricting their long-term usage, demanding newer alternative therapies. This study aimed to investigate the anti-asthmatic properties of Ruta graveolens extract and its prepared nano-cubosomal dispersion (Ruta-ND). Firstly, the R. graveolens methanolic extract exhibited higher anti-inflammatory activity on Lipopolysaccharide (LPS)-activated BEAS-2B cells. To ensure best bioavailability and hence best cellular uptake, R. graveolens extract was loaded in nano-cubosomal dispersion (ND). Then, the anti-asthmatic effects of Ruta extract and ND were simultaneously evaluated in rats’ model with ovalbumin-induced allergic asthma. R. graveolens extract and Ruta-ND subsided asthma score and improved lung function by restoring FEV1/FVC ratio to the expected values in control rats. Also, it showed strong antioxidant and anti-inflammatory activities manifested by lowering levels of malondialdehyde (MDA), IL-4, IL-7, TGF-β, and Ig-E, and increasing levels of superoxide dismutase (SOD) and INF-γ in bronchoalveolar lavage fluid. Our research findings also indicate autophagy induction and apoptosis inhibition by Ruta extract and Ruta-ND. Finally, the HPLC MS/MS phytochemical profiling of R. graveolens extract evident production of various alkaloids, flavonoids, coumarins, and other phenolics with reported pharmacological properties corresponding to/emphasize our study findings. In conclusion, R. graveolens exhibited promise in managing Ova-induced allergic asthma and could be developed as an alternative anti-allergic asthma drug. |
| first_indexed | 2024-03-07T19:42:14Z |
| format | Article |
| id | doaj.art-1874e812110e4504a19c1c123acfbb9a |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-03-07T19:42:14Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-1874e812110e4504a19c1c123acfbb9a2024-02-29T05:18:20ZengElsevierSaudi Pharmaceutical Journal1319-01642024-03-01323101968Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammationMohammad H. Alyami0Dalia I. Hamdan1Heba M.A. Khalil2Mohamed A.A. Orabi3Nora M. Aborehab4Nada Osama5Mai M. Abdelhafez6Abdulaziz Mohsen Al-Mahallawi7Hamad S. Alyami8Department of Pharmaceutics, College of Pharmacy, Najran University, Najran 66462, Saudi ArabiaDepartment of Pharmacognosy and Natural Products, Faculty of Pharmacy, Menoufia University, Shibin Elkom 32511, EgyptDepartment of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; Corresponding authors at: Department of Pharmaceutics, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia.Department of Pharmacognosy, College of Pharmacy, Najran University, Najran, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut-branch, Assiut 71524, EgyptDepartment of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, EgyptBiochemistry Department, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shibin Elkom, 32511 Menoufia, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, MSA University, EgyptDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, EgyptDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia; Corresponding authors at: Department of Pharmaceutics, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia.Asthma is a chronic disease affecting people of all ages. Asthma medications are associated with adverse effects restricting their long-term usage, demanding newer alternative therapies. This study aimed to investigate the anti-asthmatic properties of Ruta graveolens extract and its prepared nano-cubosomal dispersion (Ruta-ND). Firstly, the R. graveolens methanolic extract exhibited higher anti-inflammatory activity on Lipopolysaccharide (LPS)-activated BEAS-2B cells. To ensure best bioavailability and hence best cellular uptake, R. graveolens extract was loaded in nano-cubosomal dispersion (ND). Then, the anti-asthmatic effects of Ruta extract and ND were simultaneously evaluated in rats’ model with ovalbumin-induced allergic asthma. R. graveolens extract and Ruta-ND subsided asthma score and improved lung function by restoring FEV1/FVC ratio to the expected values in control rats. Also, it showed strong antioxidant and anti-inflammatory activities manifested by lowering levels of malondialdehyde (MDA), IL-4, IL-7, TGF-β, and Ig-E, and increasing levels of superoxide dismutase (SOD) and INF-γ in bronchoalveolar lavage fluid. Our research findings also indicate autophagy induction and apoptosis inhibition by Ruta extract and Ruta-ND. Finally, the HPLC MS/MS phytochemical profiling of R. graveolens extract evident production of various alkaloids, flavonoids, coumarins, and other phenolics with reported pharmacological properties corresponding to/emphasize our study findings. In conclusion, R. graveolens exhibited promise in managing Ova-induced allergic asthma and could be developed as an alternative anti-allergic asthma drug.http://www.sciencedirect.com/science/article/pii/S1319016424000185Ovalbumin Allergic airwayRespiratory functionAutophagyBeclin-1ApoptosisBehavior scoring |
| spellingShingle | Mohammad H. Alyami Dalia I. Hamdan Heba M.A. Khalil Mohamed A.A. Orabi Nora M. Aborehab Nada Osama Mai M. Abdelhafez Abdulaziz Mohsen Al-Mahallawi Hamad S. Alyami Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation Saudi Pharmaceutical Journal Ovalbumin Allergic airway Respiratory function Autophagy Beclin-1 Apoptosis Behavior scoring |
| title | Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation |
| title_full | Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation |
| title_fullStr | Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation |
| title_full_unstemmed | Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation |
| title_short | Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation |
| title_sort | preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with ruta graveolens extracts as a novel approach to reduce asthma mediated lung inflammation |
| topic | Ovalbumin Allergic airway Respiratory function Autophagy Beclin-1 Apoptosis Behavior scoring |
| url | http://www.sciencedirect.com/science/article/pii/S1319016424000185 |
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