Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism
Abstract Distyly, a floral dimorphism associated with heteromorphic self-incompatibility and controlled by the S-locus supergene, evolved independently multiple times. Comparative analyses of the first transcriptome atlas for the main distyly model, Primula veris, with other distylous species produc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25132-2 |
| _version_ | 1811315141360222208 |
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| author | Giacomo Potente Rebecca L. Stubbs Narjes Yousefi Walter Pirovano Péter Szövényi Elena Conti |
| author_facet | Giacomo Potente Rebecca L. Stubbs Narjes Yousefi Walter Pirovano Péter Szövényi Elena Conti |
| author_sort | Giacomo Potente |
| collection | DOAJ |
| description | Abstract Distyly, a floral dimorphism associated with heteromorphic self-incompatibility and controlled by the S-locus supergene, evolved independently multiple times. Comparative analyses of the first transcriptome atlas for the main distyly model, Primula veris, with other distylous species produced the following findings. A set of 53 constitutively expressed genes in P. veris did not include any of the housekeeping genes commonly used to normalize gene expression in qPCR experiments. The S-locus gene CYP T acquired its role in controlling style elongation via a change in expression profile. Comparison of genes differentially expressed between floral morphs revealed that brassinosteroids and auxin are the main hormones controlling style elongation in P. veris and Fagopyrum esculentum, respectively. Furthermore, shared biochemical pathways might underlie the expression of distyly in the distantly related P. veris, F. esculentum and Turnera subulata, suggesting a degree of correspondence between evolutionary convergence at phenotypic and molecular levels. Finally, we provide the first evidence supporting the previously proposed hypothesis that distyly supergenes of distantly related species evolved via the recruitment of genes related to the phytochrome-interacting factor (PIF) signaling network. To conclude, this is the first study that discovered homologous genes involved in the control of distyly in distantly related taxa. |
| first_indexed | 2024-04-13T11:26:05Z |
| format | Article |
| id | doaj.art-1875fa2ac3bf424a82cfacf9b119c622 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T11:26:05Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1875fa2ac3bf424a82cfacf9b119c6222022-12-22T02:48:42ZengNature PortfolioScientific Reports2045-23222022-12-0112111510.1038/s41598-022-25132-2Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphismGiacomo Potente0Rebecca L. Stubbs1Narjes Yousefi2Walter Pirovano3Péter Szövényi4Elena Conti5Department of Systematic and Evolutionary Botany, University of ZurichDepartment of Systematic and Evolutionary Botany, University of ZurichDepartment of Systematic and Evolutionary Botany, University of ZurichBaseClear BVDepartment of Systematic and Evolutionary Botany, University of ZurichDepartment of Systematic and Evolutionary Botany, University of ZurichAbstract Distyly, a floral dimorphism associated with heteromorphic self-incompatibility and controlled by the S-locus supergene, evolved independently multiple times. Comparative analyses of the first transcriptome atlas for the main distyly model, Primula veris, with other distylous species produced the following findings. A set of 53 constitutively expressed genes in P. veris did not include any of the housekeeping genes commonly used to normalize gene expression in qPCR experiments. The S-locus gene CYP T acquired its role in controlling style elongation via a change in expression profile. Comparison of genes differentially expressed between floral morphs revealed that brassinosteroids and auxin are the main hormones controlling style elongation in P. veris and Fagopyrum esculentum, respectively. Furthermore, shared biochemical pathways might underlie the expression of distyly in the distantly related P. veris, F. esculentum and Turnera subulata, suggesting a degree of correspondence between evolutionary convergence at phenotypic and molecular levels. Finally, we provide the first evidence supporting the previously proposed hypothesis that distyly supergenes of distantly related species evolved via the recruitment of genes related to the phytochrome-interacting factor (PIF) signaling network. To conclude, this is the first study that discovered homologous genes involved in the control of distyly in distantly related taxa.https://doi.org/10.1038/s41598-022-25132-2 |
| spellingShingle | Giacomo Potente Rebecca L. Stubbs Narjes Yousefi Walter Pirovano Péter Szövényi Elena Conti Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism Scientific Reports |
| title | Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| title_full | Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| title_fullStr | Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| title_full_unstemmed | Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| title_short | Comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| title_sort | comparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism |
| url | https://doi.org/10.1038/s41598-022-25132-2 |
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