A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean
Small heat shock proteins (sHSPs) are ubiquitous proteins present in all organisms. The sHSPs are not only upregulated under heat shock as well as other stresses but also are expressed in unstressed cells, indicating quite diverse functions of sHSPs. However, there is little known about the role of...
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| Format: | Article |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.838718/full |
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| author | Zhanwu Yang Zhanwu Yang Hui Du Hui Du Jingyi Sun Jingyi Sun Xinzhu Xing Xinzhu Xing Youbin Kong Youbin Kong Wenlong Li Wenlong Li Xihuan Li Xihuan Li Caiying Zhang Caiying Zhang |
| author_facet | Zhanwu Yang Zhanwu Yang Hui Du Hui Du Jingyi Sun Jingyi Sun Xinzhu Xing Xinzhu Xing Youbin Kong Youbin Kong Wenlong Li Wenlong Li Xihuan Li Xihuan Li Caiying Zhang Caiying Zhang |
| author_sort | Zhanwu Yang |
| collection | DOAJ |
| description | Small heat shock proteins (sHSPs) are ubiquitous proteins present in all organisms. The sHSPs are not only upregulated under heat shock as well as other stresses but also are expressed in unstressed cells, indicating quite diverse functions of sHSPs. However, there is little known about the role of sHSPs in nodulation and nitrogen fixation in soybean. In this study, we cloned a candidate protein of sHSP, GmHSP17.1, from proteome of nodule and analyzed its function in soybean nodulation. We found that GmHSP17.1 was a cytosolic protein and preferentially expressed during nodule development. An overexpression of GmHSP17.1 in composite transgenic plants showed increases in nodule number, fresh weight, nodule size, area of infection cells, and nitrogenase activity, and subsequently promoted the content of nitrogen and growth of soybean plants. While GmHSP17.1 RNA interference (RNAi) lines showed significantly impaired nodule development and nitrogen fixation efficiency. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS), GmRIP1 was identified as the first potential target of GmHSP17.1, and was shown to be specifically expressed in soybean nodules. The interaction between GmHSP17.1 and GmRIP1 was further confirmed by yeast-two hybrid (Y2H), bimolecular fluorescence complementation (BiFC) in vivo and pull-down assay in vitro. Furthermore, peroxidase activity was markedly increased in GmHSP17.1 overexpressed nodules and decreased in RNAi lines. As a result, the reactive oxygen species (ROS) content greatly decreased in GmHSP17.1 overexpression lines and increased in suppression lines. Taken together, we conclude that GmHSP17.1 plays an important role in soybean nodulation through interacting with GmRIP1. Our results provide foundation for studying the mechanism of nitrogen fixation and for the genetics improvement of legume plants. |
| first_indexed | 2024-12-10T16:40:55Z |
| format | Article |
| id | doaj.art-18763aaae5b3497e9e319f507f3eccbe |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T16:40:55Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-18763aaae5b3497e9e319f507f3eccbe2022-12-22T01:41:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.838718838718A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in SoybeanZhanwu Yang0Zhanwu Yang1Hui Du2Hui Du3Jingyi Sun4Jingyi Sun5Xinzhu Xing6Xinzhu Xing7Youbin Kong8Youbin Kong9Wenlong Li10Wenlong Li11Xihuan Li12Xihuan Li13Caiying Zhang14Caiying Zhang15State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, ChinaNorth China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, ChinaSmall heat shock proteins (sHSPs) are ubiquitous proteins present in all organisms. The sHSPs are not only upregulated under heat shock as well as other stresses but also are expressed in unstressed cells, indicating quite diverse functions of sHSPs. However, there is little known about the role of sHSPs in nodulation and nitrogen fixation in soybean. In this study, we cloned a candidate protein of sHSP, GmHSP17.1, from proteome of nodule and analyzed its function in soybean nodulation. We found that GmHSP17.1 was a cytosolic protein and preferentially expressed during nodule development. An overexpression of GmHSP17.1 in composite transgenic plants showed increases in nodule number, fresh weight, nodule size, area of infection cells, and nitrogenase activity, and subsequently promoted the content of nitrogen and growth of soybean plants. While GmHSP17.1 RNA interference (RNAi) lines showed significantly impaired nodule development and nitrogen fixation efficiency. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS), GmRIP1 was identified as the first potential target of GmHSP17.1, and was shown to be specifically expressed in soybean nodules. The interaction between GmHSP17.1 and GmRIP1 was further confirmed by yeast-two hybrid (Y2H), bimolecular fluorescence complementation (BiFC) in vivo and pull-down assay in vitro. Furthermore, peroxidase activity was markedly increased in GmHSP17.1 overexpressed nodules and decreased in RNAi lines. As a result, the reactive oxygen species (ROS) content greatly decreased in GmHSP17.1 overexpression lines and increased in suppression lines. Taken together, we conclude that GmHSP17.1 plays an important role in soybean nodulation through interacting with GmRIP1. Our results provide foundation for studying the mechanism of nitrogen fixation and for the genetics improvement of legume plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.838718/fullsmall heat shock proteins (sHSPs)soybean nodulebiological nitrogen fixation (BNF)peroxidase activitymolecular breeding |
| spellingShingle | Zhanwu Yang Zhanwu Yang Hui Du Hui Du Jingyi Sun Jingyi Sun Xinzhu Xing Xinzhu Xing Youbin Kong Youbin Kong Wenlong Li Wenlong Li Xihuan Li Xihuan Li Caiying Zhang Caiying Zhang A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean Frontiers in Plant Science small heat shock proteins (sHSPs) soybean nodule biological nitrogen fixation (BNF) peroxidase activity molecular breeding |
| title | A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean |
| title_full | A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean |
| title_fullStr | A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean |
| title_full_unstemmed | A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean |
| title_short | A Nodule-Localized Small Heat Shock Protein GmHSP17.1 Confers Nodule Development and Nitrogen Fixation in Soybean |
| title_sort | nodule localized small heat shock protein gmhsp17 1 confers nodule development and nitrogen fixation in soybean |
| topic | small heat shock proteins (sHSPs) soybean nodule biological nitrogen fixation (BNF) peroxidase activity molecular breeding |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.838718/full |
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