Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats
Objection: To investigate the potential link between aberrant mitochondrial energy metabolism mediated by the AMPK/SIRT1 pathway and the etiology of anxiety disorders. Methods: The anxiety rat model was established by uncertain empty water bottle（UEWB）stress. Rats were submitted behavioral tests on...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Brain Research Bulletin |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923023001958 |
| _version_ | 1797657858603483136 |
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| author | An-ran Zhao Jie Li Si-qi Wang Li-hua Bian Wen-jing Li Jian-you Guo |
| author_facet | An-ran Zhao Jie Li Si-qi Wang Li-hua Bian Wen-jing Li Jian-you Guo |
| author_sort | An-ran Zhao |
| collection | DOAJ |
| description | Objection: To investigate the potential link between aberrant mitochondrial energy metabolism mediated by the AMPK/SIRT1 pathway and the etiology of anxiety disorders. Methods: The anxiety rat model was established by uncertain empty water bottle（UEWB）stress. Rats were submitted behavioral tests on the seventh, fourteenth, and twenty-first days and had the prefrontal cortex and amygdala removed for biochemical tests. The morphological alterations of the mitochondria in the medial prefrontal cortex and amygdala were examined by using a transmission electron microscope. Expression levels of AMPK, SIRT1, PGC-1, NRF-1 and NRF-2 were tested by western-blot analysis. ATP, respiratory chain complex and caspase enzyme expressions were tested by neurochemical and biochemical assays. Results: Rats showed anxiety-like behavior after being exposed to the uncertain empty water bottle (UEWB) stress model. In model rats, mitochondrial structure is damaged, mitochondrial energy metabolism is decreased, and the expression of proteins associated with AMPK/SIRT1 pathway is significantly reduced in the brain. Conclusion: The level of mitochondrial energy metabolism correlates with anxiety-like behavior. The main mechanism of anxiety disorder is a disturbance of mitochondrial energy metabolism, which might be related to AMPK/SIRT1 pathway. |
| first_indexed | 2024-03-11T17:51:38Z |
| format | Article |
| id | doaj.art-1877ee886ee24155af2a68d282e88e6a |
| institution | Directory Open Access Journal |
| issn | 1873-2747 |
| language | English |
| last_indexed | 2024-03-11T17:51:38Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj.art-1877ee886ee24155af2a68d282e88e6a2023-10-18T04:30:19ZengElsevierBrain Research Bulletin1873-27472023-10-01203110770Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in ratsAn-ran Zhao0Jie Li1Si-qi Wang2Li-hua Bian3Wen-jing Li4Jian-you Guo5CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, ChinaCAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Intelligent Education Technology and Application of Zhejiang Province, Zhejiang Normal University, Jinhua Zhejiang 321004; Correspondence to: Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.Objection: To investigate the potential link between aberrant mitochondrial energy metabolism mediated by the AMPK/SIRT1 pathway and the etiology of anxiety disorders. Methods: The anxiety rat model was established by uncertain empty water bottle（UEWB）stress. Rats were submitted behavioral tests on the seventh, fourteenth, and twenty-first days and had the prefrontal cortex and amygdala removed for biochemical tests. The morphological alterations of the mitochondria in the medial prefrontal cortex and amygdala were examined by using a transmission electron microscope. Expression levels of AMPK, SIRT1, PGC-1, NRF-1 and NRF-2 were tested by western-blot analysis. ATP, respiratory chain complex and caspase enzyme expressions were tested by neurochemical and biochemical assays. Results: Rats showed anxiety-like behavior after being exposed to the uncertain empty water bottle (UEWB) stress model. In model rats, mitochondrial structure is damaged, mitochondrial energy metabolism is decreased, and the expression of proteins associated with AMPK/SIRT1 pathway is significantly reduced in the brain. Conclusion: The level of mitochondrial energy metabolism correlates with anxiety-like behavior. The main mechanism of anxiety disorder is a disturbance of mitochondrial energy metabolism, which might be related to AMPK/SIRT1 pathway.http://www.sciencedirect.com/science/article/pii/S0361923023001958AnxietyMitochondrial functionEnergy metabolismAMPK/SIRT1 pathways |
| spellingShingle | An-ran Zhao Jie Li Si-qi Wang Li-hua Bian Wen-jing Li Jian-you Guo Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats Brain Research Bulletin Anxiety Mitochondrial function Energy metabolism AMPK/SIRT1 pathways |
| title | Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats |
| title_full | Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats |
| title_fullStr | Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats |
| title_full_unstemmed | Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats |
| title_short | Stress can affect mitochondrial energy metabolism and AMPK/SIRT1 signaling pathway in rats |
| title_sort | stress can affect mitochondrial energy metabolism and ampk sirt1 signaling pathway in rats |
| topic | Anxiety Mitochondrial function Energy metabolism AMPK/SIRT1 pathways |
| url | http://www.sciencedirect.com/science/article/pii/S0361923023001958 |
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