An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility
Background and Objectives: One of the public concerns is emergence of Vancomycin-resistant Enterococci. VanA and vanB genes is responsible for resistance to high concentrations of Vancomycin. The purpose of this study is detection of the vanA and vanB genes in the Enterococcus faecalisand Enterococc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2015-05-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
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| Online Access: | http://journal.muq.ac.ir/article-1-377-en.html |
| _version_ | 1818434973306191872 |
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| author | Hamed Samadi Rahim Pirhajati Mahabadi Abazar Pournajaf Sajad Omidi Somayeh Moghimyan Neda Alyasin |
| author_facet | Hamed Samadi Rahim Pirhajati Mahabadi Abazar Pournajaf Sajad Omidi Somayeh Moghimyan Neda Alyasin |
| author_sort | Hamed Samadi |
| collection | DOAJ |
| description | Background and Objectives: One of the public concerns is emergence of Vancomycin-resistant Enterococci. VanA and vanB genes is responsible for resistance to high concentrations of Vancomycin. The purpose of this study is detection of the vanA and vanB genes in the Enterococcus faecalisand Enterococcus faecium isolated from hospitalized patients in Shariati hospital and their antibiotic susceptibility profile.
Methods: A total of 228 samples, including urine, blood, wound and stool were collected from patients referred to the Shariati hospital. Suspected grown colonies were identified by standard methods and then antibiotic susceptibility testing by disk diffusion assay performed according to Clinical and Laboratory Standards Institute (CLSI). E. test was used to determination of MIC levels on the samples with resistance to Vancomycin. Distribution of vanA and vanB genes was determined by using of PCR method.
Results: In this study, out of 113 achieved Enterococcus, the highest and lowest prevalence of E. faecalis and E. faecium were 103 (91.1%) and 10 (8.9%), respectively. In the disk diffusion method, 11 (7/9%) strains were resistant to Vancomycin. All Vancomycin resistance isolates revealed vanA, but not vanB.
Conclusion: The increase of Vancomycin resistance strains is a serious threat in the world and our country. Furthermore, the limited treatment options for patients with Enterococcus infections caused increasing cost and transfer resistance genes to other bacteria |
| first_indexed | 2024-12-14T16:45:30Z |
| format | Article |
| id | doaj.art-187cc5d0a2654c579a0048ae859801f0 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-14T16:45:30Z |
| publishDate | 2015-05-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-187cc5d0a2654c579a0048ae859801f02022-12-21T22:54:11ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752015-05-01933238An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic SusceptibilityHamed Samadi0Rahim Pirhajati Mahabadi1Abazar Pournajaf2Sajad Omidi3Somayeh Moghimyan4Neda Alyasin5 Azad Islamic University Qom University of Medical Sciences Iran University of Medical Sciences Iran University of Medical Sciences Azad Islamic University, Iran University of Medical Sciences Background and Objectives: One of the public concerns is emergence of Vancomycin-resistant Enterococci. VanA and vanB genes is responsible for resistance to high concentrations of Vancomycin. The purpose of this study is detection of the vanA and vanB genes in the Enterococcus faecalisand Enterococcus faecium isolated from hospitalized patients in Shariati hospital and their antibiotic susceptibility profile. Methods: A total of 228 samples, including urine, blood, wound and stool were collected from patients referred to the Shariati hospital. Suspected grown colonies were identified by standard methods and then antibiotic susceptibility testing by disk diffusion assay performed according to Clinical and Laboratory Standards Institute (CLSI). E. test was used to determination of MIC levels on the samples with resistance to Vancomycin. Distribution of vanA and vanB genes was determined by using of PCR method. Results: In this study, out of 113 achieved Enterococcus, the highest and lowest prevalence of E. faecalis and E. faecium were 103 (91.1%) and 10 (8.9%), respectively. In the disk diffusion method, 11 (7/9%) strains were resistant to Vancomycin. All Vancomycin resistance isolates revealed vanA, but not vanB. Conclusion: The increase of Vancomycin resistance strains is a serious threat in the world and our country. Furthermore, the limited treatment options for patients with Enterococcus infections caused increasing cost and transfer resistance genes to other bacteriahttp://journal.muq.ac.ir/article-1-377-en.htmldrug resistanceenterococcus faecalisvana;ampvanb proteinenterococcus faeciumpolymerase chain reaction |
| spellingShingle | Hamed Samadi Rahim Pirhajati Mahabadi Abazar Pournajaf Sajad Omidi Somayeh Moghimyan Neda Alyasin An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum drug resistance enterococcus faecalis vana;amp vanb protein enterococcus faecium polymerase chain reaction |
| title | An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility |
| title_full | An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility |
| title_fullStr | An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility |
| title_full_unstemmed | An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility |
| title_short | An Investigation of the vanA and vanB Genes in Enterococcus faecalis and Enterococcus faecium Strains Isolated from the Hospitalized Patients in Shariati Hospital and Evaluation of Their Antibiotic Susceptibility |
| title_sort | investigation of the vana and vanb genes in enterococcus faecalis and enterococcus faecium strains isolated from the hospitalized patients in shariati hospital and evaluation of their antibiotic susceptibility |
| topic | drug resistance enterococcus faecalis vana;amp vanb protein enterococcus faecium polymerase chain reaction |
| url | http://journal.muq.ac.ir/article-1-377-en.html |
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