Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium
Enterococcus faecium, is an important nosocomial pathogen with increased incidence of multidrug resistance (MDR) – specifically Vancomycin resistance. E. faecium constitutes the normal microbiota of the human intestine as well as exists in the hospitals and sewage, thus making the microorganism diff...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2018-12-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2018-0066 |
| _version_ | 1818689571360079872 |
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| author | Raza Taskeen Andleeb Saadia Ullah Sidra Rahmat Jamal Muhsin Mehmood Khalid Ali Muhammad |
| author_facet | Raza Taskeen Andleeb Saadia Ullah Sidra Rahmat Jamal Muhsin Mehmood Khalid Ali Muhammad |
| author_sort | Raza Taskeen |
| collection | DOAJ |
| description | Enterococcus faecium, is an important nosocomial pathogen with increased incidence of multidrug resistance (MDR) – specifically Vancomycin resistance. E. faecium constitutes the normal microbiota of the human intestine as well as exists in the hospitals and sewage, thus making the microorganism difficult to eliminate. Phage therapy has gained attention for controlling bacterial MDR infections and contaminations. We have successfully isolated from waste water and characterized a lytic bacteriophage STH1 capable of targeting Vancomycin resistant Enterococcus faecium (VREF) with high specificity. The phage was isolated from sewage water of a hospital at district Dera Ismail Khan, Pakistan. Initial characterization showed that magnesium and calcium ions significantly increased phage adsorption to the host. One step growth experiment showed a latent period of 18 min with burst size of 334 virions per cell. Optimal temperature and pH of the phage was 37°C and 7.0, respectively. Phage application to host strain grown in milk and water (treated and untreated) showed that the phage efficiently controlled bacterial growth. The study suggests that the phage STH1 can serve as potential control agent for E. faecium infections in medical facilities and in other environmental contaminations. |
| first_indexed | 2024-12-17T12:12:13Z |
| format | Article |
| id | doaj.art-187d5a26124142cb94123b3dd5603b96 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-17T12:12:13Z |
| publishDate | 2018-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-187d5a26124142cb94123b3dd5603b962022-12-21T21:49:20ZengDe GruyterOpen Life Sciences2391-54122018-12-0113155356010.1515/biol-2018-0066biol-2018-0066Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faeciumRaza Taskeen0Andleeb Saadia1Ullah Sidra Rahmat2Jamal Muhsin3Mehmood Khalid4Ali Muhammad5Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, PakistanAtta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, PakistanAtta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, PakistanDepartment of Microbiology, Abdul Wali Khan University, Mardan-23200, Mardan, PakistanDepartment of Pharmacy, Abbottabad University of Science and Technology, Havelian, PakistanDepartment of Life Sciences, School of Science, University of Management and Technology, C-II Johar Town, Lahore 54770, PakistanEnterococcus faecium, is an important nosocomial pathogen with increased incidence of multidrug resistance (MDR) – specifically Vancomycin resistance. E. faecium constitutes the normal microbiota of the human intestine as well as exists in the hospitals and sewage, thus making the microorganism difficult to eliminate. Phage therapy has gained attention for controlling bacterial MDR infections and contaminations. We have successfully isolated from waste water and characterized a lytic bacteriophage STH1 capable of targeting Vancomycin resistant Enterococcus faecium (VREF) with high specificity. The phage was isolated from sewage water of a hospital at district Dera Ismail Khan, Pakistan. Initial characterization showed that magnesium and calcium ions significantly increased phage adsorption to the host. One step growth experiment showed a latent period of 18 min with burst size of 334 virions per cell. Optimal temperature and pH of the phage was 37°C and 7.0, respectively. Phage application to host strain grown in milk and water (treated and untreated) showed that the phage efficiently controlled bacterial growth. The study suggests that the phage STH1 can serve as potential control agent for E. faecium infections in medical facilities and in other environmental contaminations.https://doi.org/10.1515/biol-2018-0066enterococcus faeciumphage therapyvancomycin resistance |
| spellingShingle | Raza Taskeen Andleeb Saadia Ullah Sidra Rahmat Jamal Muhsin Mehmood Khalid Ali Muhammad Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium Open Life Sciences enterococcus faecium phage therapy vancomycin resistance |
| title | Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium |
| title_full | Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium |
| title_fullStr | Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium |
| title_full_unstemmed | Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium |
| title_short | Isolation and Characterization of a Phage to Control Vancomycin Resistant Enterococcus faecium |
| title_sort | isolation and characterization of a phage to control vancomycin resistant enterococcus faecium |
| topic | enterococcus faecium phage therapy vancomycin resistance |
| url | https://doi.org/10.1515/biol-2018-0066 |
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