Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study
<i>Zingiber officinale</i> Roscoe and <i>Citrus limon</i> L. are well known for their multi-use and for their pharmacological effect in the treatment of many illnesses. This study aims to investigate the chemical composition of the ginger and lemon juice extracts and in addit...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/2/390 |
| _version_ | 1797483179228004352 |
|---|---|
| author | Oussama Bekkouch Mohammed Dalli Mohamed Harnafi Ilham Touiss Imane Mokhtari Soufiane El Assri Hicham Harnafi Mohammed Choukri Seok-Jae Ko Bonglee Kim Souliman Amrani |
| author_facet | Oussama Bekkouch Mohammed Dalli Mohamed Harnafi Ilham Touiss Imane Mokhtari Soufiane El Assri Hicham Harnafi Mohammed Choukri Seok-Jae Ko Bonglee Kim Souliman Amrani |
| author_sort | Oussama Bekkouch |
| collection | DOAJ |
| description | <i>Zingiber officinale</i> Roscoe and <i>Citrus limon</i> L. are well known for their multi-use and for their pharmacological effect in the treatment of many illnesses. This study aims to investigate the chemical composition of the ginger and lemon juice extracts and in addition, to evaluate their antioxidant properties and their hepatoprotective effect against the liver damage of Wistar rats induced by the injection of CCl<sub>4</sub> to treated animals. The obtained effects were completed by a histological study for better confirmation of the registered pharmacological effects. The ginger juice extract was found to be rich in 4-gingerol, 6-gingediol, and 6-gingerol, while the lemon juice extract chemical composition was highlighted by the presence of eriodyctiol, rutin, hesperidin, and isorhamnetin. Concerning the antioxidant activity, the ginger, lemon juice extracts, and their formulation showed an important antioxidant potential using TAC (total antioxidant capacity), an antiradical activity against the radical DPPH• (2,2-diphenyl-1-picrylhydrazil), and a ferric reducing power. Finally, the ginger, lemon, and their formulation at different doses were able to prevent CCl<sub>4</sub> induced liver damage. Indeed, these different bioactive compounds could be used as alternative agents for the treatment of chronic liver diseases. |
| first_indexed | 2024-03-09T22:43:14Z |
| format | Article |
| id | doaj.art-187df26c476d44c8abbbbbb217a51bdb |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:43:14Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-187df26c476d44c8abbbbbb217a51bdb2023-11-23T18:33:04ZengMDPI AGAntioxidants2076-39212022-02-0111239010.3390/antiox11020390Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological StudyOussama Bekkouch0Mohammed Dalli1Mohamed Harnafi2Ilham Touiss3Imane Mokhtari4Soufiane El Assri5Hicham Harnafi6Mohammed Choukri7Seok-Jae Ko8Bonglee Kim9Souliman Amrani10Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Biochemistry, University Hospital Center Mohammed VI, BP 4806, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Biochemistry, University Hospital Center Mohammed VI, BP 4806, Oujda 60000, MoroccoDepartment of Gastroenterology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, Morocco<i>Zingiber officinale</i> Roscoe and <i>Citrus limon</i> L. are well known for their multi-use and for their pharmacological effect in the treatment of many illnesses. This study aims to investigate the chemical composition of the ginger and lemon juice extracts and in addition, to evaluate their antioxidant properties and their hepatoprotective effect against the liver damage of Wistar rats induced by the injection of CCl<sub>4</sub> to treated animals. The obtained effects were completed by a histological study for better confirmation of the registered pharmacological effects. The ginger juice extract was found to be rich in 4-gingerol, 6-gingediol, and 6-gingerol, while the lemon juice extract chemical composition was highlighted by the presence of eriodyctiol, rutin, hesperidin, and isorhamnetin. Concerning the antioxidant activity, the ginger, lemon juice extracts, and their formulation showed an important antioxidant potential using TAC (total antioxidant capacity), an antiradical activity against the radical DPPH• (2,2-diphenyl-1-picrylhydrazil), and a ferric reducing power. Finally, the ginger, lemon, and their formulation at different doses were able to prevent CCl<sub>4</sub> induced liver damage. Indeed, these different bioactive compounds could be used as alternative agents for the treatment of chronic liver diseases.https://www.mdpi.com/2076-3921/11/2/390<i>Zingiber officinale</i><i>Citrus limon</i>synergyantioxidanthepatoprotectionCCl<sub>4</sub> |
| spellingShingle | Oussama Bekkouch Mohammed Dalli Mohamed Harnafi Ilham Touiss Imane Mokhtari Soufiane El Assri Hicham Harnafi Mohammed Choukri Seok-Jae Ko Bonglee Kim Souliman Amrani Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study Antioxidants <i>Zingiber officinale</i> <i>Citrus limon</i> synergy antioxidant hepatoprotection CCl<sub>4</sub> |
| title | Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study |
| title_full | Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study |
| title_fullStr | Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study |
| title_full_unstemmed | Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study |
| title_short | Ginger (<i>Zingiber officinale</i> Roscoe), Lemon (<i>Citrus limon</i> L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl<sub>4</sub>: A Biochemical and Histological Study |
| title_sort | ginger i zingiber officinale i roscoe lemon i citrus limon i l juices as preventive agents from chronic liver damage induced by ccl sub 4 sub a biochemical and histological study |
| topic | <i>Zingiber officinale</i> <i>Citrus limon</i> synergy antioxidant hepatoprotection CCl<sub>4</sub> |
| url | https://www.mdpi.com/2076-3921/11/2/390 |
| work_keys_str_mv | AT oussamabekkouch gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT mohammeddalli gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT mohamedharnafi gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT ilhamtouiss gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT imanemokhtari gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT soufianeelassri gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT hichamharnafi gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT mohammedchoukri gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT seokjaeko gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT bongleekim gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy AT soulimanamrani gingerizingiberofficinaleiroscoelemonicitruslimoniljuicesaspreventiveagentsfromchronicliverdamageinducedbycclsub4subabiochemicalandhistologicalstudy |