The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina
Background/Aim: Warfarin is one of the most common orally prescribed anticoagulant in patients with deep venous thrombosis, myocardial or cerebral infarctions. The main side effects of non-adequate dose of these drugs are prolonged peripheral or internal bleeding. VKORC1 1173C>T polymorphism (rs9...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Medical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of Medicine
2023-01-01
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| Series: | Scripta Medica |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2023/2490-33292302175V.pdf |
| _version_ | 1797787793194221568 |
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| author | Vidović Vanja Bećarević Jelena Radić-Savić Žana Marić Aljoša Vidović Stojko Milovac Irina Maksimović Nela |
| author_facet | Vidović Vanja Bećarević Jelena Radić-Savić Žana Marić Aljoša Vidović Stojko Milovac Irina Maksimović Nela |
| author_sort | Vidović Vanja |
| collection | DOAJ |
| description | Background/Aim: Warfarin is one of the most common orally prescribed anticoagulant in patients with deep venous thrombosis, myocardial or cerebral infarctions. The main side effects of non-adequate dose of these drugs are prolonged peripheral or internal bleeding. VKORC1 1173C>T polymorphism (rs9934438) is of particular importance, since carriers of non-wild type allele correlates with the lower dosage of warfarin therapy. Thus, the aim of the research was to determine the distribution of 1173C>T polymorphism in population of the Republic of Srpska, Bosnia and Herzegovina (RS) and to compare results with frequencies in other populations. Methods: A total of 124 healthy participants of both genders were enrolled in the study, from all parts of the RS. Molecular genotyping was performed by real-time PCR, using drug metabolism assays according to the manufacturer's instructions. Results: Of the total number, 22 subjects (17.74 %) were genotyped as CC, 69 subjects (55.65 %) as CT and 33 subjects (26.61 %) as TT. The frequencies of alleles C and T were 45.18 % and 54.82 %, respectively. No statistical significance was found among allele distribution between genders (ch² = 0.236; p = 0.627). All observed genotype frequencies were in Hardy-Weinberg equilibrium. No statistical significance was observed among the frequency of minor T allele between presented findings and other European countries, besides Russia (p = 0.021). Conclusion: This was the first study analysing the distribution of rs9934438 alleles in population of the RS. These findings will be helpful in better and more precise drug prescribing in patients who require anticoagulant therapy. |
| first_indexed | 2024-03-13T01:27:48Z |
| format | Article |
| id | doaj.art-187dfd55d07f428f9ecdb924092b54a5 |
| institution | Directory Open Access Journal |
| issn | 2490-3329 2303-7954 |
| language | English |
| last_indexed | 2024-03-13T01:27:48Z |
| publishDate | 2023-01-01 |
| publisher | Medical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of Medicine |
| record_format | Article |
| series | Scripta Medica |
| spelling | doaj.art-187dfd55d07f428f9ecdb924092b54a52023-07-04T10:50:17ZengMedical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of MedicineScripta Medica2490-33292303-79542023-01-015421751792490-33292302175VThe prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and HerzegovinaVidović Vanja0Bećarević Jelena1Radić-Savić Žana2Marić Aljoša3Vidović Stojko4Milovac Irina5Maksimović Nela6https://orcid.org/0000-0002-5815-573XUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Centre for Biomedical Research, Banja Luka, the Republic of Srpska, Bosnia and HerzegovinaUniversity of Belgrade, Faculty of Medicine, Institute of Human Genetics, Belgrade, SerbiaBackground/Aim: Warfarin is one of the most common orally prescribed anticoagulant in patients with deep venous thrombosis, myocardial or cerebral infarctions. The main side effects of non-adequate dose of these drugs are prolonged peripheral or internal bleeding. VKORC1 1173C>T polymorphism (rs9934438) is of particular importance, since carriers of non-wild type allele correlates with the lower dosage of warfarin therapy. Thus, the aim of the research was to determine the distribution of 1173C>T polymorphism in population of the Republic of Srpska, Bosnia and Herzegovina (RS) and to compare results with frequencies in other populations. Methods: A total of 124 healthy participants of both genders were enrolled in the study, from all parts of the RS. Molecular genotyping was performed by real-time PCR, using drug metabolism assays according to the manufacturer's instructions. Results: Of the total number, 22 subjects (17.74 %) were genotyped as CC, 69 subjects (55.65 %) as CT and 33 subjects (26.61 %) as TT. The frequencies of alleles C and T were 45.18 % and 54.82 %, respectively. No statistical significance was found among allele distribution between genders (ch² = 0.236; p = 0.627). All observed genotype frequencies were in Hardy-Weinberg equilibrium. No statistical significance was observed among the frequency of minor T allele between presented findings and other European countries, besides Russia (p = 0.021). Conclusion: This was the first study analysing the distribution of rs9934438 alleles in population of the RS. These findings will be helpful in better and more precise drug prescribing in patients who require anticoagulant therapy.https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2023/2490-33292302175V.pdfvkorc1allele distributionpolymorphismwarfarin |
| spellingShingle | Vidović Vanja Bećarević Jelena Radić-Savić Žana Marić Aljoša Vidović Stojko Milovac Irina Maksimović Nela The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina Scripta Medica vkorc1 allele distribution polymorphism warfarin |
| title | The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina |
| title_full | The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina |
| title_fullStr | The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina |
| title_full_unstemmed | The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina |
| title_short | The prevalence of VKORC1 alleles in the population of the Republic of Srpska, Bosnia and Herzegovina |
| title_sort | prevalence of vkorc1 alleles in the population of the republic of srpska bosnia and herzegovina |
| topic | vkorc1 allele distribution polymorphism warfarin |
| url | https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2023/2490-33292302175V.pdf |
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