Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines
Objective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG2 cell lines with different toxicants viz, CCL4, Ethanol and Paracetamol with different concentrations of th...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2019-03-01
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| Series: | Journal of Pharmacopuncture |
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| Online Access: | http://www.journal-pharm.com/sub/view/274 |
| _version_ | 1811314054373834752 |
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| author | Chitra Jairaman Syed Ali Mohamed Yacoob Anuradha Venkatraman Yogananth Nagarajan Gnanadesigan Murugesan |
| author_facet | Chitra Jairaman Syed Ali Mohamed Yacoob Anuradha Venkatraman Yogananth Nagarajan Gnanadesigan Murugesan |
| author_sort | Chitra Jairaman |
| collection | DOAJ |
| description | Objective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG2 cell lines with different toxicants viz, CCL4, Ethanol and Paracetamol with different concentrations of the extract were used. The HepG2 cell lines were subjected to MTT Assay for studying the cytotoxicity. Methods: HepG2 cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% CCl4, 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and 300 μg/ml) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm. Results: The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, 300 μg/ml. The results for the CCl4 intoxicated, at 300 μg/ml of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was 21.53 μg/ml, 12.61 μg/ml and 21.42 μg/ml respectively. Conclusion: Thus, we conclude that, the extract possesses defensive effect against different toxicants and can be used as an alternate drug for hepatotoxicity. |
| first_indexed | 2024-04-13T11:05:10Z |
| format | Article |
| id | doaj.art-1883d92fc281446892d19a1bc0228b2c |
| institution | Directory Open Access Journal |
| issn | 2093-6966 |
| language | English |
| last_indexed | 2024-04-13T11:05:10Z |
| publishDate | 2019-03-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-1883d92fc281446892d19a1bc0228b2c2022-12-22T02:49:17ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662019-03-0122104104810.3831/KPI.2019.22.005KPI.2019.22.005Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell LinesChitra Jairaman0Syed Ali Mohamed Yacoob1Anuradha Venkatraman2Yogananth Nagarajan3Gnanadesigan Murugesan4PG and Research Department of Biotechnology, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, IndiaPG and Research Department of Biotechnology, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, IndiaPG and Research Department of Biochemistry, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, IndiaPG and Research Department of Biotechnology, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, IndiaDepartment of Microbial Biotechnology, Bharathair University, Coimbatore-641046, Tamil nadu, IndiaObjective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG2 cell lines with different toxicants viz, CCL4, Ethanol and Paracetamol with different concentrations of the extract were used. The HepG2 cell lines were subjected to MTT Assay for studying the cytotoxicity. Methods: HepG2 cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% CCl4, 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and 300 μg/ml) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm. Results: The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, 300 μg/ml. The results for the CCl4 intoxicated, at 300 μg/ml of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was 21.53 μg/ml, 12.61 μg/ml and 21.42 μg/ml respectively. Conclusion: Thus, we conclude that, the extract possesses defensive effect against different toxicants and can be used as an alternate drug for hepatotoxicity.http://www.journal-pharm.com/sub/view/274cytotoxicityethanol: water (3:1) extracttoxicantsviable cells |
| spellingShingle | Chitra Jairaman Syed Ali Mohamed Yacoob Anuradha Venkatraman Yogananth Nagarajan Gnanadesigan Murugesan Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines Journal of Pharmacopuncture cytotoxicity ethanol: water (3:1) extract toxicants viable cells |
| title | Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines |
| title_full | Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines |
| title_fullStr | Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines |
| title_full_unstemmed | Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines |
| title_short | Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines |
| title_sort | propugnating effect of bark of rhizophora mucronata against different toxicants viz carbon tetrachloride ethanol and paracetamol on hepg2 cell lines |
| topic | cytotoxicity ethanol: water (3:1) extract toxicants viable cells |
| url | http://www.journal-pharm.com/sub/view/274 |
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