New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov)
Duckweeds have been increasingly studied in recent years, both as model plants and in view of their potential applications as a new crop in a circular bioeconomy perspective. In order to select species and clones with the desired attributes, the correct identification of the species is fundamental....
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MDPI AG
2021-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2767 |
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| author | Luca Braglia Diego Breviario Silvia Gianì Floriana Gavazzi Jacopo De Gregori Laura Morello |
| author_facet | Luca Braglia Diego Breviario Silvia Gianì Floriana Gavazzi Jacopo De Gregori Laura Morello |
| author_sort | Luca Braglia |
| collection | DOAJ |
| description | Duckweeds have been increasingly studied in recent years, both as model plants and in view of their potential applications as a new crop in a circular bioeconomy perspective. In order to select species and clones with the desired attributes, the correct identification of the species is fundamental. Molecular methods have recently provided a more solid base for taxonomy and yielded a consensus phylogenetic tree, although some points remain to be elucidated. The duckweed genus <i>Lemna</i> L. comprises twelve species, grouped in four sections, which include very similar sister species. The least taxonomically resolved is sect. <i>Lemna</i>, presenting difficulties in species delimitation using morphological and even barcoding molecular markers. Ambiguous species boundaries between <i>Lemna minor</i> L. and <i>Lemna japonica</i> Landolt have been clarified by Tubulin Based Polymorphism (TBP), with the discovery of interspecific hybrids. In the present work, we extended TBP profiling to a larger number of clones in sect. <i>Lemna</i>, previously classified using only morphological features, in order to test that classification, and to investigate the possible existence of other hybrids in this section. The analysis revealed several misidentifications of clones, in particular among the species <i>L. minor</i>, <i>L. japonica</i> and <i>Lemna gibba</i> L., and identified six putative ‘<i>L. gibba</i>’ clones as interspecific hybrids between <i>L. minor</i> and <i>L. gibba</i>. |
| first_indexed | 2024-03-10T03:16:13Z |
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| id | doaj.art-1886aba85aa54f608459c7a94c1d8a65 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:16:13Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1886aba85aa54f608459c7a94c1d8a652023-11-23T10:12:54ZengMDPI AGPlants2223-77472021-12-011012276710.3390/plants10122767New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov)Luca Braglia0Diego Breviario1Silvia Gianì2Floriana Gavazzi3Jacopo De Gregori4Laura Morello5Istituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyIstituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyIstituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyIstituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyIstituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyIstituto Biologia e Biotecnologia Agraria, Via Alfonso Corti 12, 20131 Milano, ItalyDuckweeds have been increasingly studied in recent years, both as model plants and in view of their potential applications as a new crop in a circular bioeconomy perspective. In order to select species and clones with the desired attributes, the correct identification of the species is fundamental. Molecular methods have recently provided a more solid base for taxonomy and yielded a consensus phylogenetic tree, although some points remain to be elucidated. The duckweed genus <i>Lemna</i> L. comprises twelve species, grouped in four sections, which include very similar sister species. The least taxonomically resolved is sect. <i>Lemna</i>, presenting difficulties in species delimitation using morphological and even barcoding molecular markers. Ambiguous species boundaries between <i>Lemna minor</i> L. and <i>Lemna japonica</i> Landolt have been clarified by Tubulin Based Polymorphism (TBP), with the discovery of interspecific hybrids. In the present work, we extended TBP profiling to a larger number of clones in sect. <i>Lemna</i>, previously classified using only morphological features, in order to test that classification, and to investigate the possible existence of other hybrids in this section. The analysis revealed several misidentifications of clones, in particular among the species <i>L. minor</i>, <i>L. japonica</i> and <i>Lemna gibba</i> L., and identified six putative ‘<i>L. gibba</i>’ clones as interspecific hybrids between <i>L. minor</i> and <i>L. gibba</i>.https://www.mdpi.com/2223-7747/10/12/2767duckweedsLemnaceaeinterspecific hybridstubulin-based polymorphismβ-tubulin |
| spellingShingle | Luca Braglia Diego Breviario Silvia Gianì Floriana Gavazzi Jacopo De Gregori Laura Morello New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) Plants duckweeds Lemnaceae interspecific hybrids tubulin-based polymorphism β-tubulin |
| title | New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) |
| title_full | New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) |
| title_fullStr | New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) |
| title_full_unstemmed | New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) |
| title_short | New Insights into Interspecific Hybridization in <i>Lemna</i> L. Sect. <i>Lemna</i> (Lemnaceae Martinov) |
| title_sort | new insights into interspecific hybridization in i lemna i l sect i lemna i lemnaceae martinov |
| topic | duckweeds Lemnaceae interspecific hybrids tubulin-based polymorphism β-tubulin |
| url | https://www.mdpi.com/2223-7747/10/12/2767 |
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