miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1
Abstract RAF1 (c‐raf) has been known as an important tumor‐promoter in many cancers. However, the regulatory mechanisms affecting RAF1 expressions are rarely reported. This study aimed to predict the candidate miRNAs of RAF1 gene and verify their functions in the progression of lung cancer. The expr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
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| Series: | Precision Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/prm2.12102 |
| _version_ | 1797809960120221696 |
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| author | Hao Tian Kai Ding Zhongchao Wang Li Yin Jianzhong Wu Xia He |
| author_facet | Hao Tian Kai Ding Zhongchao Wang Li Yin Jianzhong Wu Xia He |
| author_sort | Hao Tian |
| collection | DOAJ |
| description | Abstract RAF1 (c‐raf) has been known as an important tumor‐promoter in many cancers. However, the regulatory mechanisms affecting RAF1 expressions are rarely reported. This study aimed to predict the candidate miRNAs of RAF1 gene and verify their functions in the progression of lung cancer. The expression of miR‐643 was detected by reverse transcription polymerase chain reaction (RT‐PCR). A dual‐luciferase report system was used to verify the relationship between miR‐643 and RAF1. RT‐PCR and Western blot were used to analyze the regulatory relationship between miR‐643 and RAF1. Transwell chamber, scratch, and monoclonal tests showed that miR‐643 affected the proliferation, migration, and radiosensitivity of H1299 and A549 cells by targeting the RAF1 gene. The expression of miR‐643 in lung cancer cells was lower than that in the bronchial epithelioid cells (CRL‐2741), and luciferase reporter experiment confirmed that miR‐643 targeted RAF1. MiR‐643 overexpression decreased the RAF1 expression, thereby decreasing cell migration, proliferation, and radiation resistance through the AKT/nuclear factor‐κB pathway. miR‐643 in lung cancer cells could inhibit cell proliferation, invasion, and metastasis and increase the radiosensitivity by downregulating RAF1. |
| first_indexed | 2024-03-13T07:00:48Z |
| format | Article |
| id | doaj.art-188757dbb8664d929cfc6b2a59472090 |
| institution | Directory Open Access Journal |
| issn | 2642-2514 |
| language | English |
| last_indexed | 2024-03-13T07:00:48Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Precision Medical Sciences |
| spelling | doaj.art-188757dbb8664d929cfc6b2a594720902023-06-06T22:32:51ZengWileyPrecision Medical Sciences2642-25142023-06-0112211312010.1002/prm2.12102miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1Hao Tian0Kai Ding1Zhongchao Wang2Li Yin3Jianzhong Wu4Xia He5Department of Radiotherapy Xinyi People's Hospital Xu Zhou Jiangsu ChinaThe Affiliated Cancer Hospital of Nanjing Medical University Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research Nanjing Jiangsu ChinaDepartment of Radiotherapy Xinyi People's Hospital Xu Zhou Jiangsu ChinaThe Affiliated Cancer Hospital of Nanjing Medical University Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research Nanjing Jiangsu ChinaThe Affiliated Cancer Hospital of Nanjing Medical University Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research Nanjing Jiangsu ChinaThe Affiliated Cancer Hospital of Nanjing Medical University Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research Nanjing Jiangsu ChinaAbstract RAF1 (c‐raf) has been known as an important tumor‐promoter in many cancers. However, the regulatory mechanisms affecting RAF1 expressions are rarely reported. This study aimed to predict the candidate miRNAs of RAF1 gene and verify their functions in the progression of lung cancer. The expression of miR‐643 was detected by reverse transcription polymerase chain reaction (RT‐PCR). A dual‐luciferase report system was used to verify the relationship between miR‐643 and RAF1. RT‐PCR and Western blot were used to analyze the regulatory relationship between miR‐643 and RAF1. Transwell chamber, scratch, and monoclonal tests showed that miR‐643 affected the proliferation, migration, and radiosensitivity of H1299 and A549 cells by targeting the RAF1 gene. The expression of miR‐643 in lung cancer cells was lower than that in the bronchial epithelioid cells (CRL‐2741), and luciferase reporter experiment confirmed that miR‐643 targeted RAF1. MiR‐643 overexpression decreased the RAF1 expression, thereby decreasing cell migration, proliferation, and radiation resistance through the AKT/nuclear factor‐κB pathway. miR‐643 in lung cancer cells could inhibit cell proliferation, invasion, and metastasis and increase the radiosensitivity by downregulating RAF1.https://doi.org/10.1002/prm2.12102invasionlung cancermiR‐643radiosensitivityRAF1 |
| spellingShingle | Hao Tian Kai Ding Zhongchao Wang Li Yin Jianzhong Wu Xia He miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 Precision Medical Sciences invasion lung cancer miR‐643 radiosensitivity RAF1 |
| title | miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 |
| title_full | miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 |
| title_fullStr | miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 |
| title_full_unstemmed | miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 |
| title_short | miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1 |
| title_sort | mir 643 suppresses cell invasion and radioresistant of lung cancer through raf1 |
| topic | invasion lung cancer miR‐643 radiosensitivity RAF1 |
| url | https://doi.org/10.1002/prm2.12102 |
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