Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome
Polymorphisms and mutations in the surfactant protein B (SP-B) gene have been associated with the pathogenesis of respiratory distress syndrome (RDS). The objective of the present study was to compare the frequencies of SP-B gene polymorphisms between preterm babies with RDS and healthy term newborn...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2007-06-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600005 |
| _version_ | 1828487943402029056 |
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| author | P.P.R. Lyra F.A.C. Vaz P.E. Moreira J.W. Hoffmann D.E. de Mello E.M.A. Diniz |
| author_facet | P.P.R. Lyra F.A.C. Vaz P.E. Moreira J.W. Hoffmann D.E. de Mello E.M.A. Diniz |
| author_sort | P.P.R. Lyra |
| collection | DOAJ |
| description | Polymorphisms and mutations in the surfactant protein B (SP-B) gene have been associated with the pathogenesis of respiratory distress syndrome (RDS). The objective of the present study was to compare the frequencies of SP-B gene polymorphisms between preterm babies with RDS and healthy term newborns. We studied 50 preterm babies with RDS (inclusion criteria - newborns with RDS and gestational age between 28 and 33 weeks and 6 days), and 100 healthy term newborns. Four SP-B gene polymorphisms were analyzed: A/C at nucleotide -18, C/T at nucleotide 1580, A/G at nucleotide 9306, and G/C at nucleotide 8714, by PCR amplification of genomic DNA and genotyping by cRFLP. The healthy newborns comprised 42 female and 58 male neonates; 39 were white and 61 non-white. The RDS group comprised 21 female and 29 male preterm neonates; 28 were white and 22 non-white. Weight ranged from 640 to 2080 g (mean: 1273 g); mean gestational age was 31 weeks and 2 days (range: 28-33 weeks and 6 days). When white children were analyzed separately, a statistically significant difference in the G/C polymorphism at 8714 was observed between groups (P = 0.028). All other genotype frequencies were similar for both groups when sex and race were analyzed together. Analysis of the SP-B polymorphism G/C at nucleotide 8714 showed that among white neonates the GG genotype was found only in the RDS group at a frequency of 17% and the GC genotype was more frequently found in healthy term newborns. These data demonstrate an association of GG genotype with RDS. |
| first_indexed | 2024-12-11T10:00:16Z |
| format | Article |
| id | doaj.art-18879a6a10094eceb989a33c375fe1d0 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-11T10:00:16Z |
| publishDate | 2007-06-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-18879a6a10094eceb989a33c375fe1d02022-12-22T01:12:08ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2007-06-01406779786Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndromeP.P.R. LyraF.A.C. VazP.E. MoreiraJ.W. HoffmannD.E. de MelloE.M.A. DinizPolymorphisms and mutations in the surfactant protein B (SP-B) gene have been associated with the pathogenesis of respiratory distress syndrome (RDS). The objective of the present study was to compare the frequencies of SP-B gene polymorphisms between preterm babies with RDS and healthy term newborns. We studied 50 preterm babies with RDS (inclusion criteria - newborns with RDS and gestational age between 28 and 33 weeks and 6 days), and 100 healthy term newborns. Four SP-B gene polymorphisms were analyzed: A/C at nucleotide -18, C/T at nucleotide 1580, A/G at nucleotide 9306, and G/C at nucleotide 8714, by PCR amplification of genomic DNA and genotyping by cRFLP. The healthy newborns comprised 42 female and 58 male neonates; 39 were white and 61 non-white. The RDS group comprised 21 female and 29 male preterm neonates; 28 were white and 22 non-white. Weight ranged from 640 to 2080 g (mean: 1273 g); mean gestational age was 31 weeks and 2 days (range: 28-33 weeks and 6 days). When white children were analyzed separately, a statistically significant difference in the G/C polymorphism at 8714 was observed between groups (P = 0.028). All other genotype frequencies were similar for both groups when sex and race were analyzed together. Analysis of the SP-B polymorphism G/C at nucleotide 8714 showed that among white neonates the GG genotype was found only in the RDS group at a frequency of 17% and the GC genotype was more frequently found in healthy term newborns. These data demonstrate an association of GG genotype with RDS.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600005PolymorphismsSurfactant protein BRespiratory distress syndrome |
| spellingShingle | P.P.R. Lyra F.A.C. Vaz P.E. Moreira J.W. Hoffmann D.E. de Mello E.M.A. Diniz Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome Brazilian Journal of Medical and Biological Research Polymorphisms Surfactant protein B Respiratory distress syndrome |
| title | Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| title_full | Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| title_fullStr | Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| title_full_unstemmed | Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| title_short | Comparison of surfactant protein B polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| title_sort | comparison of surfactant protein b polymorphisms of healthy term newborns with preterm newborns having respiratory distress syndrome |
| topic | Polymorphisms Surfactant protein B Respiratory distress syndrome |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600005 |
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